Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
J Urban Health ; 89(3): 519-26, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22391983

ABSTRACT

During the last decade, the veterinary anesthetics have gained popularity as recreational drugs. The aim of this study was to document the use of "anestecia de caballo" (xylazine) and its consequences among drug users in Puerto Rico. The study combined a cross-sectional survey with 89 drug users and two focus groups conducted in Mayagüez with frontline drug treatment providers. Drug users were recruited from communities of the San Juan metropolitan area using a variety of ethnographic and outreach strategies. A short questionnaire developed for the study collected information on sociodemographics, xylazine use, and its consequences. The two focus groups were conducted to discuss the details related to xylazine use, its consequences, and utilization awareness. The sample comprised 63 males (70.8%) and 26 females with a mean age of 37.2 years. The mean number of years of drug use was 14.3, with a mean frequency of drug use of 5.9 times daily. More than 65% reported speedball as the principal drug of use. The prevalence of xylazine use was 80.7%. More than 42% of the sample used xylazine in a mixture with speedball. The main route of administration of xylazine was injection but 14% reported the use of xylazine by inhalation. More than 35% of the sample reported skin lesions and 21.1% reported at least one overdose episode. Multiple logistic regression analysis revealed that males (OR = 3.47, CI = 1.10-12.00) and those who reported speedball as their main drug of use (OR = 9.34, CI = 2.51-34.70) were significantly more likely to be xylazine users. Focus groups revealed that drug users claimed to recognize the presence of xylaxine in a mixture of speedball based on its effects, taste, the color of the drug (dark brown), and its odor. In conclusion, the use of xylazine among drug users in Puerto Rico seems to be an emerging trend with potentially serious health consequences.


Subject(s)
Hypnotics and Sedatives , Substance Abuse, Intravenous/epidemiology , Substance-Related Disorders/epidemiology , Xylazine , Administration, Inhalation , Adult , Cocaine/administration & dosage , Cross-Sectional Studies , Drug Combinations , Female , Focus Groups , Heroin/administration & dosage , Humans , Male , Middle Aged , Prevalence , Puerto Rico/epidemiology , Skin Ulcer/etiology , Surveys and Questionnaires , Young Adult
2.
Vet Pathol ; 47(2): 298-305, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20106793

ABSTRACT

Ten spontaneous mammary gland tumors affecting guinea pigs (GP) were analyzed histologically and immunohistochemically. Histologically, 3 were benign (2 simple adenomas and 1 benign mixed tumor) and 7 were malignant (1 simple solid carcinoma and 6 simple tubulopapillary carcinomas). Immunohistochemical data revealed the glandular immunoprofile of all the tumors and suggested their ductal origin on the basis of cytokeratin 20 expression. Interestingly, cytokeratin 7 was detected in basal/myoepithelial cells. Further, all tumors were positive for type alpha estrogen and progesterone receptors, suggesting a role for steroid hormones in the development of these neoplasias in GP. This article describes the morphological and immunohistochemical features of the normal mammary gland and spontaneous mammary gland tumors in GP.


Subject(s)
Adenocarcinoma/veterinary , Guinea Pigs , Mammary Neoplasms, Animal/pathology , Rodent Diseases/pathology , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Animals , Estrogen Receptor alpha/metabolism , Female , Immunohistochemistry/veterinary , Male , Mammary Neoplasms, Animal/metabolism , Receptors, Progesterone/metabolism , Retrospective Studies , Rodent Diseases/metabolism
3.
Vet Pathol ; 42(3): 250-7, 2005 May.
Article in English | MEDLINE | ID: mdl-15872371

ABSTRACT

Maspin is a serine protease inhibitor that inhibits tumor invasion and metastasis in human breast cancer and is consistently expressed by mammary myoepithelial cells (MECs). To analyze the value of maspin as a marker of the MEC layer of the normal and tumoral canine mammary gland, the immunohistochemical expression of maspin was studied in formalin-fixed tissues from 55 benign and malignant tumors (40 tumors also contained the surrounding normal mammary gland) using a commercially available monoclonal antibody. Periacinar and periductal MECs of all 40 normal mammary glands were stained by the anti-human maspin monoclonal antibody, and immunoreactivity was observed in the nucleus and cytoplasm of these cells. In addition, maspin was found in 53 (98%) of the tumors studied, reacting with the MECs in 100% of benign tumors and 93% of malignant tumors and to the epithelial cells of 16% of benign and 73% of malignant tumors. In the MEC compartment, immunoreactivity was observed in the cytoplasm of hypertrophic MECs, fusiform MECs, stellate MECs, rounded (myoepithelial) cells, and chondroblasts. In the epithelial cell compartment, immunoreactivity was observed in the cytoplasm of cells with and without squamous differentiation. Stromal myofibroblasts were unreactive. Maspin appears to be a very sensitive marker of the normal and neoplastic myoepithelium that, contrary to smooth muscle differentiation markers, does not stain stromal myofibroblasts. In addition, a subset of neoplastic epithelial cells reacted with the maspin antibody. The relationship between maspin expression in different cellular compartments of canine mammary carcinomas and the biologic aggressiveness of the disease remains to be elucidated.


Subject(s)
Dog Diseases/metabolism , Mammary Neoplasms, Animal/metabolism , Myoepithelioma/veterinary , Serpins/metabolism , Animals , Antibodies, Monoclonal , Dogs , Genes, Tumor Suppressor , Immunohistochemistry , Myoepithelioma/metabolism
4.
Vet Pathol ; 41(3): 269-72, 2004 May.
Article in English | MEDLINE | ID: mdl-15133176

ABSTRACT

To date, two isoforms of estrogen receptors (ER) have been identified, cloned, and characterized from several species, estrogen receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta). Although the presence of ERalpha has been demonstrated in normal and tumoral canine mammary tissues, the issue of ERbeta expression has not been addressed in the dog. In this study, we have analyzed the expression of ERbeta in formalin-fixed, paraffin-embedded tissue samples of nonaltered mammary gland, 30 malignant (six complex carcinoma, 12 simple carcinoma, three carcinosarcoma, and nine carcinoma or sarcoma in benign tumor), and five benign (one fibroadenoma, one complex papilloma, one complex adenoma, and two benign mixed tumors) mammary tumors of the dog by using a polyclonal ERbeta antibody and the avidin-biotin-peroxidase complex immunohistochemical technique. Our results show that high numbers of normal ductal and acinar epithelium and approximately one third of canine mammary tumors express ERbeta. This expression was higher in benign than in malignant tumors. Furthermore, expression was higher in complex and mixed histologic subtypes of malignant tumors when compared with simple subtypes.


Subject(s)
Avidin/analogs & derivatives , Dog Diseases/metabolism , Dog Diseases/pathology , Gene Expression Regulation, Neoplastic , Mammary Neoplasms, Animal/metabolism , Neoplasms, Glandular and Epithelial/veterinary , Receptors, Estrogen/metabolism , Sarcoma/veterinary , Animals , Biotin , Dogs , Estrogen Receptor beta , Female , Horseradish Peroxidase , Immunohistochemistry , Mammary Neoplasms, Animal/pathology , Neoplasms, Glandular and Epithelial/metabolism , Neoplasms, Glandular and Epithelial/pathology , Ovary/pathology , Sarcoma/metabolism , Sarcoma/pathology , Uterus/pathology
5.
Vet Pathol ; 39(6): 739-43, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12450207

ABSTRACT

Spontaneous basaloid adenomas occurred in four out of 354 dogs with mammary tumors. Affected dogs were pure-bred, intact females between 6 and 8 years of age. Three dogs were nuliparous, two had pseudopregnancies, and none had received contraceptive steroids. The tumors were multiple (three cases) or unique, less than 1 cm in diameter, well delineated, and composed of uniform cords and clusters of monomorphic epithelial cells with focal signs of squamous or glandular differentiation. A basal cell immunophenotype (cytokeratins 5 and 14 positive) without either glandular epithelial (cytokeratins 8, 18, and 19 negative) or myoepithelial (calponin negative) differentiation was observed in the majority of tumor cells. No recurrence or metastasis was recorded after follow-up periods between 3 and 24 months. In spite of the hormone-dependent nature of this tumor in female Beagles given experimental contraceptive steroids, spontaneous basaloid adenomas lacked estrogen receptor alpha and progesterone receptors.


Subject(s)
Adenoma/veterinary , Dog Diseases/pathology , Mammary Neoplasms, Animal/pathology , Adenoma/metabolism , Adenoma/pathology , Animals , Calcium-Binding Proteins/metabolism , Dog Diseases/metabolism , Dogs , Female , Immunohistochemistry/veterinary , Keratins/metabolism , Mammary Neoplasms, Animal/metabolism , Microfilament Proteins , Calponins
6.
Vet Pathol ; 39(2): 247-56, 2002 Mar.
Article in English | MEDLINE | ID: mdl-12009063

ABSTRACT

The immunohistochemical expression of the smooth muscle-specific protein calponin was studied to assess the contribution of myoepithelial cells to the histogenesis of spindle cells of complex and mixed tumors of the mammary gland of the dog and the origin of cartilage and bone in mixed tumors. Formalin-fixed tissues from 55 benign and malignant tumors (49 also containing surrounding normal mammary gland) were evaluated. Periacinar and periductal myoepithelial cells of all the 49 normal mammary glands were diffusely stained by the anti-human calponin monoclonal antibody. Calponin was found in 53 (98%) of the tumors studied, reacting with the myoepithelium-like cells of 86% of benign tumors and their remnants in 85% of malignant tumors. Five different types of calponin-immunoreactive myoepithelial cells were identified: hypertrophic myoepithelial cells. fusiform cells, stellate myoepithelial cells, rounded (myoepithelial) cells, and chondroblasts. Differences in staining intensity and staining pattern among these five types of cells suggested a transition of myoepithelial cells to chondroblasts. Stromal myofibroblasts also showed calponin immunoreactivity, but they did not react with a cytokeratin 14 monoclonal antibody, which recognizes myoepithelial cells in mammary gland. Calponin appears to be a very sensitive marker of normal and neoplastic myoepithelium in the canine mammary gland, and its identification in different cell types of complex and mixed tumors of the mammary gland of the dog suggests a major histogenetic role for myoepithelial cells.


Subject(s)
Calcium-Binding Proteins/metabolism , Dog Diseases/metabolism , Mammary Neoplasms, Animal/metabolism , Muscle, Smooth/metabolism , Animals , Antibodies, Monoclonal , Dog Diseases/pathology , Dogs , Epithelium/metabolism , Epithelium/pathology , Female , Immunoenzyme Techniques/veterinary , Immunohistochemistry/veterinary , Mammary Glands, Animal/metabolism , Mammary Glands, Animal/pathology , Mammary Neoplasms, Animal/pathology , Microfilament Proteins , Muscle, Smooth/pathology , Calponins
7.
Vet Pathol ; 36(3): 179-90, 1999 May.
Article in English | MEDLINE | ID: mdl-10332826

ABSTRACT

Forty-seven feline and 60 canine epithelial tumors were studied to test the coordinate expression of cytokeratin 7 (CK 7) and cytokeratin 20 (CK 20) using commercially available monoclonal antibodies and an avidin-biotin immunoperoxidase staining technique. Previously, the distribution of both cytokeratins was examined in normal tissues from 4 cats and 4 dogs. The pattern of distribution of CK 7 in normal tissues was similar, with minor differences, to that described in humans, whereas the reactivity pattern of CK 20 in cats and dogs was wider than that in humans. The subset of tumors strongly expressing CK 7 and CK 20 included pancreatic adenocarcinomas (100%), transitional cell carcinomas (75%), and endometrial carcinomas (67%) in the cat. None of the canine tumors had this immunophenotype. Feline (50%) and canine (56%) mammary gland carcinomas and canine cholangiocarcinomas (67%) were the only tumors presenting the CK 7 +/CK 20- immunophenotype, whereas the CK 7-/CK 20+ immunophenotype included thyroid carcinomas (100%), intestinal adenocarcinomas (60%), bronchioloalveolar carcinomas (50%), and renal carcinomas (50%) in the cat and intestinal adenocarcinomas (56%), gastric adenocarcinomas (50%), and ovarian carcinomas (50%) in the dog. The CK 7-/CK 20- immunophenotype included the rest of the analyzed tumors. The immunohistochemical evaluation of coordinate expression of both CK 7 and CK 20 in feline and canine carcinomas using monoclonal antibodies provides important information that can help to discriminate among carcinomas from different primary sites and could be particularly helpful in the determination of the primary site of origin of carcinomas presenting as metastatic disease.


Subject(s)
Carcinoma/veterinary , Cat Diseases/pathology , Dog Diseases/pathology , Gene Expression Regulation, Neoplastic , Intermediate Filament Proteins/genetics , Keratins/genetics , Animals , Antibodies, Monoclonal , Carcinoma/chemistry , Carcinoma/pathology , Cat Diseases/diagnosis , Cats , Dog Diseases/diagnosis , Dogs , Female , Immunohistochemistry , Intermediate Filament Proteins/analysis , Intestine, Large/chemistry , Intestine, Large/pathology , Keratin-20 , Keratin-7 , Keratins/analysis , Liver/chemistry , Liver/pathology , Male , Mammary Glands, Animal/chemistry , Mammary Glands, Animal/pathology , Ovary/chemistry , Ovary/pathology , Pancreatic Neoplasms/chemistry , Pancreatic Neoplasms/pathology , Pancreatic Neoplasms/veterinary , Pituitary Gland/chemistry , Pituitary Gland/pathology , Reference Values , Stomach/chemistry , Stomach/pathology , Tongue/chemistry , Tongue/pathology , Urinary Bladder/chemistry , Urinary Bladder/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...