Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Oral Biol ; 54(2): 185-91, 2009 Feb.
Article in English | MEDLINE | ID: mdl-18980757

ABSTRACT

OBJECTIVE: Vascular endothelial growth factor (VEGF) has been implicated in the regulation of dental pulp and dentine repair. Therapeutic ultrasound was shown to be effective for fracture repair. We investigated whether low frequency ultrasound influences the production of VEGF by odontoblast-like cells. Moreover, we examined the direct effects of VEGF on odontoblast-like cell proliferation. DESIGN: MDPC-23, an established odontoblast-like cell line, was exposed to increasing intensities of 30kHz ultrasound using an ultrasonic tip probe. RESULTS: After 24h cell culture, WST-1 analysis of cell viability and number showed a dose-dependent decrease in the number of viable cells with increasing ultrasound power. However, the relative concentration of VEGF as analysed by ELISA and normalised to cell number was significantly increased in the culture supernatants indicating an ultrasound-induced stimulation of odontoblastic VEGF secretion. Analysis of VEGF gene expression by sqRT-PCR revealed the expression of the main VEGF isoforms in the MDPC-23 cells, i.e. VEGF(120) and VEGF(164) as well as to a minor extent VEGF(188). Low power ultrasound increased gene expression of all VEGF isoforms. Addition of recombinant VEGF to the cell cultures significantly stimulated cell proliferation. Gene expression of the VEGF receptors Flt1/VEGFR1 and KDR/VEGFR2 was detected in the MDPC-23, suggesting the possibility that VEGF may act on the odontoblast-like cells in an autocrine manner. CONCLUSIONS: Our results indicate that ultrasound promoted VEGF expression and production by odontoblast-like cells and that VEGF may have autocrine effects on these cells. It is proposed that ultrasound may influence odontoblast activity and dentine repair by modulating production of endogenous growth factors in the dentine-pulp complex.


Subject(s)
Odontoblasts/metabolism , Ultrasonic Therapy , Vascular Endothelial Growth Factor A/biosynthesis , Animals , Cell Line , Cell Proliferation/drug effects , Gene Expression Regulation , Mice , Odontoblasts/cytology , Odontoblasts/drug effects , Recombinant Proteins/pharmacology , Reverse Transcriptase Polymerase Chain Reaction/methods , Vascular Endothelial Growth Factor A/genetics , Vascular Endothelial Growth Factor A/pharmacology
2.
Clin Exp Immunol ; 148(2): 307-24, 2007 May.
Article in English | MEDLINE | ID: mdl-17355248

ABSTRACT

To investigate the molecular effects of the periodontopathogens Fusobacterium nucleatum (FN) and Porphyromonas gingivalis (PG) on the oral epithelium, the H400 oral epithelial cell line was cultured in the presence of non-viable bacteria. Following confirmation of the presence of transcripts for the bacterial pattern recognition receptors in H400 cells, Toll-like receptors -2, -4 and -9, and components of the NF-kappaB signalling pathway, immunocytochemical analyses were performed showing that NF-kappaB was activated within 1 h of exposure to both periodontopathogens. A significantly greater number of NF-kappaB nuclear translocations were apparent following H400 cell exposure to FN as compared with PG. Gene expression analyses indicated that transcripts known to be regulated by the NF-kappaB pathway, including cytokines/chemokines TNF-alpha, IL-1beta, IL-8, MCP-1/CCL2 and GM-CSF, were up-regulated following 4 and 24 h of exposure to both periodontopathogens. In addition, H400 periodontopathogen exposure resulted in differential regulation of transcripts for several cytokeratin gene family members. Consistent with the immunocytochemical data, microarray results indicated that FN induced a greater number of gene expression changes than PG following 24 h of exposure, 609 and 409 genes, respectively. Ninety-one genes were commonly differentially expressed by both periodontopathogens and represented biological processes commonly associated with periodontitis. Gene expression analyses by reserve transcriptase-polymerase chain reaction (RT-PCR) of molecules identified from the microarray data sets, including Heme oxygenase-1, lysyl oxidase, SOD2, CCL20 and calprotectin components, confirmed their differential expression profiles induced by the two periodontopathogens. FN and PG have clearly different molecular effects on oral epithelial cells, potentially highlighting the importance of the composition of the plaque biofilm in periodontitis pathogenesis.


Subject(s)
Mouth Mucosa/metabolism , NF-kappa B/metabolism , Periodontitis/metabolism , Bacteroidaceae Infections/metabolism , Bacteroidaceae Infections/pathology , Cell Line , Chemokines/biosynthesis , Chemokines/genetics , Epithelial Cells/metabolism , Fusobacterium Infections/metabolism , Fusobacterium Infections/pathology , Fusobacterium nucleatum , Gene Expression Regulation , Humans , Oligonucleotide Array Sequence Analysis/methods , Periodontitis/genetics , Periodontitis/microbiology , Periodontitis/pathology , Porphyromonas gingivalis , Reverse Transcriptase Polymerase Chain Reaction/methods , Signal Transduction , Up-Regulation
SELECTION OF CITATIONS
SEARCH DETAIL
...