Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
J Phys Chem B ; 117(41): 12442-50, 2013 Oct 17.
Article in English | MEDLINE | ID: mdl-24053455

ABSTRACT

Infrared (IR) spectroscopic imaging of human liver tissue slices has been used to identify and characterize liver tumors. Liver tissue, containing a liver metastasis of breast origin (mucinous carcinoma), was surgically removed from a consenting patient and frozen without formalin fixation or dehydration procedures, so that lipids and water remained in the tissues. A set of IR metrics (ratios of various IR peaks) was determined for tumors in fixation-free liver tissues. K-means cluster analysis was used to tell tumor from nontumor. In this case, there was a large reduction in lipid content upon going from nontumor to tumor tissue, and a well-resolved IR spectrum of nontumor liver lipid was obtained and analyzed. These IR metrics may someday guide work on IR spectroscopic diagnostics on patients in the operating room. This work also suggests utility for these methods beyond the identification of liver tumors, perhaps in the study of liver lipids.


Subject(s)
Liver Neoplasms/diagnosis , Humans , Liver Neoplasms/chemistry , Software , Spectroscopy, Fourier Transform Infrared
2.
PLoS One ; 6(7): e22638, 2011.
Article in English | MEDLINE | ID: mdl-21818356

ABSTRACT

Normal human premenopausal cervical tissue has been used to derive primary cell populations and to establish ex vivo organ culture systems to study infections with herpes simplex virus (HSV-1 or HSV-2) and human immunodeficiency virus type 1 (HIV-1). Infection with either HSV-1 or HSV-2 rapidly induced multinuclear giant cell formation and widespread damage in mucosal epithelial cells. Subsequent exposure of the damaged mucosal surfaces to HIV-1 revealed frequent co-localization of HSV and HIV-1 antigens. The short-term organ culture system provides direct experimental support for the epidemiological findings that pre-existing sexually transmitted infections, including primary and recurrent herpes virus infections at mucosal surfaces, represent major risk factors for acquisition of primary HIV-1 infection. Epithelial damage in combination with pre-existing inflammation, as described here for overtly normal human premenopausal cervix, creates a highly susceptible environment for the initiation and establishment of primary HIV-1 infection in the sub-mucosa of the cervical transformation zone.


Subject(s)
Cervix Uteri/pathology , Cervix Uteri/virology , Epithelium/pathology , Epithelium/virology , HIV Infections/virology , HIV-1/physiology , Simplexvirus/physiology , Antigens, Viral/immunology , Cell Aggregation , Cells, Cultured , Disease Susceptibility , Epithelial Cells/pathology , Epithelial Cells/virology , Female , Fibroblasts/pathology , Fibroblasts/virology , Giant Cells/pathology , Giant Cells/virology , HIV Infections/complications , HIV Infections/pathology , HIV-1/immunology , Humans , Immunohistochemistry , Inflammation/complications , Inflammation/pathology , Mucous Membrane/pathology , Mucous Membrane/virology , Organ Culture Techniques , Premenopause , Simplexvirus/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...