ABSTRACT
Morphological and chemical attributes of diaspores in myrmecochorous plants have been shown to affect seed dispersal by ants, but the relative importance of these attributes in determining seed attractiveness and dispersal success is poorly understood. We explored whether differences in diaspore morphology, elaiosome fatty acids, or elaiosome phytochemical profiles explain the differential attractiveness of five species in the genus Trillium to eastern North American forest ants. Species were ranked from least to most attractive based on empirically-derived seed dispersal probabilities in our study system, and we compared diaspore traits to test our hypotheses that more attractive species will have larger diaspores, greater concentrations of elaiosome fatty acids, and distinct elaiosome phytochemistry compared to the less attractive species. Diaspore length, width, mass, and elaiosome length were significantly greater in the more attractive species. Using gas chromatography-mass spectrometry, we found significantly higher concentrations of oleic, linoleic, hexadecenoic, stearic, palmitoleic, and total fatty acids in elaiosomes of the more attractive species. Multivariate assessments revealed that elaiosome phytochemical profiles, identified through liquid chromatography-mass spectrometry, were more homogeneous for the more attractive species. Random forest classification models (RFCM) identified several elaiosome phytochemicals that differed significantly among species. Random forest regression models revealed that some of the compounds identified by RFCM, including methylhistidine (α-amino acid) and d-glucarate (carbohydrate), were positively related to seed dispersal probabilities, while others, including salicylate (salicylic acid) and citrulline (L-α-amino acid), were negatively related. These results supported our hypotheses that the more attractive species of Trillium-which are geographically widespread compared to their less attractive, endemic congeners-are characterized by larger diaspores, greater concentrations of fatty acids, and distinct elaiosome phytochemistry. Further advances in our understanding of seed dispersal effectiveness in myrmecochorous systems will benefit from a portrayal of dispersal unit chemical and physical traits, and their combined responses to selection pressures.
ABSTRACT
In the title compound, C19H13IO2, the dihedral angle between the naphthyl ring system and the pendant iodo-phenyl ring is 72.48â (11)°. In the crystal, C-Hâ¯π inter-actions and Iâ¯O [3.293â (2)â Å] halogen bonds are observed, which combine to generate a herringbone packing motif.
ABSTRACT
PREMISE OF THE STUDY: Comparing ecological attributes of endemic species with related, widespread species can reveal differences accounting for rarity. Forests of the southeastern United States are home to many range-restricted endemic and widespread species of Trillium, a genus of ant-dispersed herbs. Evidence suggests that aspects of seed-related life history stages are often correlated with plant rarity, but few studies have tested whether the process of seed dispersal differs for endemic and widespread species. To address this question, we compared aspects of seed dispersal effectiveness (SDE) for three sympatric, widespread endemic Trillium species pairs. METHODS: We observed seed dispersal for Trillium species pairs by ants at eight sites, recorded numbers of seeds dispersed and dispersal distances, and described disperser interactions. To test disperser preference, we presented seeds of each pair to captive colonies of Aphaenogaster picea, a keystone disperser. Seeds were assigned scores based on worker behavior, and we recorded proportions of seeds dispersed after 1 h and 24 h. KEY RESULTS: Field observations yielded some significant within-pair differences. Ants dispersed more seeds of widespread species for all pairs, although dispersal distances did not differ. In laboratory experiments, after 24 h, ants dispersed more seeds of widespread species into nests. CONCLUSIONS: Endemic Trillium species had lower overall SDE than did their widespread congeners. These findings add to the list of ecological and demographic challenges that face endemic plants when compared to common congeners. Lower SDE may negatively impact reproductive rates and the colonization of new habitats, which may contribute to patterns of endemism.
