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1.
J Hosp Palliat Nurs ; 26(2): E74-E82, 2024 Apr 01.
Article in English | MEDLINE | ID: mdl-37962185

ABSTRACT

Pediatric palliative care was introduced in South Korea in 2018, with an increased need for care of children with life-limiting conditions, such as leukemia. However, the perspective of parents, who are the primary caregivers, regarding pediatric palliative care has not been explored. This study aimed to describe the pediatric palliative care-related experiences of parents who had lost a child to leukemia to better understand their needs and care outcomes. Ten mothers of children who received pediatric palliative care were recruited. Individual in-depth interviews were conducted. Phenomenology was applied to elucidate parents' experiences during their children's illness and after bereavement. From 179 main statements and 46 meaningful units, 22 themes were derived and grouped into 11 theme clusters and 4 categories. The participants described that the pediatric palliative care team had an indispensable role in providing emotional support to them and their children; this support continued even after the child's death. In addition, the participants were satisfied with their choice to receive pediatric palliative care and hoped that more regions could benefit from the services. The study findings could contribute to advances and the popularization of pediatric palliative care in South Korea.


Subject(s)
Bereavement , Leukemia , Female , Child , Humans , Palliative Care/psychology , Parents/psychology , Republic of Korea
2.
Res Nurs Health ; 47(1): 17-26, 2024 Feb.
Article in English | MEDLINE | ID: mdl-37982320

ABSTRACT

Presenteeism, which refers to going to work despite being ill, has been associated with loss of work productivity, decreased quality of care, and an increased risk of health problems. The prevalence of presenteeism is particularly high among nurses; therefore, a reliable and valid tool is needed to assess presenteeism. This study aimed to translate the Stanford Presenteeism Scale (SPS) and to examine its psychometric properties in a Korean nurse population. A methodological study was performed in January 2023. The original SPS-6 (English-language) was translated and adapted to the Korean language using a committee approach translation, cognitive interviews, and content validation with a review panel of experts. An online survey was completed by 608 Korean nurses. The internal consistency, item-to-total correlation, construct validity, concurrent validity, and discriminant validity were examined as psychometric properties of the Korean version of the SPS. The final version of the K-SPS consists of four items shortened from the original six items. Cronbach's alpha of the K-SPS-4 was 0.74, indicating adequate reliability. Confirmatory factor analysis showed a one-factor solution with a good fit. There was a positive correlation between the K-SPS-4 and the Work Limitations Questionnaire. Discriminant validity was evidenced by the positive correlation of the K-SPS-4 with job satisfaction and the negative correlation with job stress. The K-SPS-4 has acceptable psychometric properties, supporting the feasibility of its use in assessing the level of presenteeism among Korean nurses. The validated K-SPS-4 can provide a basis for developing intervention programs to enhance productivity and promote health among nurses in clinical settings.


Subject(s)
Health Promotion , Presenteeism , Humans , Psychometrics , Reproducibility of Results , Surveys and Questionnaires , Language , Republic of Korea
3.
Nurs Ethics ; 30(2): 276-287, 2023 Mar.
Article in English | MEDLINE | ID: mdl-36314069

ABSTRACT

BACKGROUND: As Korean neonatal nurses frequently experience the deaths of infants, moral distress occurs when they provide end-of-life care to the infants and their families. Although they need to care for the patients' deaths and consequently experience burnout and turnover due to moral distress from the situation, there is a lack of a support for nurses. Moreover, not much information is available on the moral distress of neonatal nurses. There is a need to better understand Korean neonatal nurses' moral distress to develop and implement appropriate supports. OBJECTIVE: This study aimed to describe nurses' experience of moral distress when they provide end-of-life care to infants and their families in neonatal intensive care units. RESEARCH DESIGN: This is a secondary analysis qualitative study. Content analysis was performed based on Corley's theory of moral distress to develop a codebook and identify themes regarding moral distress among the nurses. PARTICIPANTS AND RESEARCH CONTEXT: Qualitative data were collected from 20 nurses working in two NICUs in Seoul, South Korea. ETHICAL CONSIDERATIONS: The original study obtained permission from a university's institutional review board (IRB). This secondary analysis study obtained the exemption from another university's IRB. Nurses' participation was voluntary and confidential. FINDINGS: The nurses' moral distress was derived when they faced moral constraints and/or moral conflicts. Two distinct categories of moral constraints and four distinct categories of moral conflicts were identified among the neonatal nurses. In addition, impacts of moral distress on patients and nurses were identified. CONCLUSIONS: This study identified occasions neonatal nurses experience moral distress, and thus can guide in developing and implementing effective interventions to decrease their moral distress and improve their resilience in end-of-life care by providing insight into neonatal nurses' needs for support in end-of-life care.


Subject(s)
Nurses, Neonatal , Nurses , Infant, Newborn , Humans , Infant , Intensive Care Units, Neonatal , Attitude of Health Personnel , Stress, Psychological/complications , Morals , Surveys and Questionnaires
4.
J Pediatr Nurs ; 65: e93-e98, 2022.
Article in English | MEDLINE | ID: mdl-35422366

ABSTRACT

PURPOSE: Depressive symptoms among adolescents are a major concern for increased risk of adverse health condition outcomes later in life. Specifically, previous studies have reported that multicultural adolescents experience more depressive symptoms than non-multicultural ones. Using the Korean Children and Youth Panel Survey, this study examined the relationships between ego-resilience, social support, and depression among multicultural adolescents in South Korea, as well as the moderating effect of social support on the association between ego-resilience and depression. DESIGN AND METHODS: A total of 1500 multicultural adolescents were included in the analyses, where generalized estimating equation models were employed. RESULTS: Depression scores increased slightly from 2012 to 2015. Higher levels of ego-resilience, family support, and friend support were associated with lower levels of depression, while teacher support was not a significant predictor. The moderating effect of friends and family support on the association between ego-resilience and depression was not statistically significant. CONCLUSIONS: To reduce the level of depression among multicultural adolescents, future research should develop interventions that enhance ego-resilience ability, as well as necessary social systems that strengthen family and friend support. PRACTICE IMPLICATIONS: Supportive interventions, such as enhancing ego-resilience to reduce depression should be provided to multicultural adolescents. It is recommended to include family and friends to develop effective intervention programs for multicultural adolescents who are suffering from depression.


Subject(s)
Friends , Social Support , Adolescent , Child , Ego , Humans , Republic of Korea , Surveys and Questionnaires
5.
Nurse Educ Today ; 108: 105178, 2022 Jan.
Article in English | MEDLINE | ID: mdl-34717098

ABSTRACT

OBJECTIVES: To systematically summarize research employing serious games in nurse education, to examine their effectiveness, to provide recommendations and implementation strategies, and to suggest future directions for the development and application of serious games in nurse education. DESIGN: A systematic review. DATA SOURCES: An online search of the CINAHL, Medline, PubMed, EMBASE, PsycINFO, SCOPUS, and Web of Science databases, and a manual search of the reference lists of selected studies or review articles published in English and Korean between 1990 and July 2020. REVIEW METHODS: This systematic review was conducted in accordance with the Cochrane Handbook for Systematic Reviews of Interventions. Articles that employed a serious game approach in nurse education were included. Two authors independently screened and reviewed the articles and assessed the methodological quality using the Cochrane risk-of-bias tools. RESULTS: A total of seven studies met the inclusion criteria: three randomized controlled trials and four quasi-experimental studies. Heterogeneity was found across all studies regarding the application of serious games, platforms, and gamification elements. Most of the studies evaluated the effectiveness of serious games using knowledge tests, while two studies evaluated skills performance. Two randomized controlled trials and two one group before and after studies reported that the use of serious games improved nursing students' and nurses' knowledge and performance. CONCLUSIONS: This systematic review does not provide comprehensive insights into the effectiveness of serious games in nurse education. However, based on the evidence reviewed, we provide suggestions for developing and implementing serious games in nurse education to enhance students' knowledge and performance.


Subject(s)
Education, Nursing , Nurses , Students, Nursing , Humans , Clinical Competence , Gamification
6.
Article in English | MEDLINE | ID: mdl-33322086

ABSTRACT

Gender differences in alcohol use have narrowed over the decades. This study aimed to explore changes in alcohol consumption patterns between 2007 and 2016 to identify gender convergence in alcohol use in Korea. Data from the Korea National Health and Nutrition Examination Survey were used. For all respondents (41,662 girls/women and 32,041 boys/men) aged ≥12 years, lifetime drinking, current drinking, age of drinking onset, heavy alcohol use, and binge drinking were analyzed. Gender differences in heavy alcohol use and binge drinking decreased from 2007 to 2016 (p = 0.001 and p < 0.001, respectively). The prevalence of heavy alcohol use and binge drinking decreased in boys/men (67.5% to 63.9%, p = 0.001; 63.4% to 60.9%, p = 0.001, respectively), but not in girls/women (50.2% to 50.4%, p = 0.279; 30.6% to 32.0%, p = 0.994, respectively). The proportion of lifetime abstainers decreased among both girls/women (24.3% to 19.1%, p < 0.001) and boys/men (12.1% to 9.7%, p = 0.01). In girls/women, the mean age of drinking onset decreased (from 24.1 to 23.6 years, p = 0.017); however, in boys/men, significant changes were not observed (from 18.9 to 18.7 years, p = 0.307). Healthcare providers should be aware of the growing health risks resulting from increased unhealthy alcohol use in women and develop gender-specific preventive interventions.


Subject(s)
Alcohol Drinking/epidemiology , Sex Factors , Adolescent , Adult , Aged , Asian People , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Nutrition Surveys , Pregnancy , Prevalence , Republic of Korea/epidemiology , Young Adult
7.
Nurs Health Sci ; 22(2): 254-262, 2020 Jun.
Article in English | MEDLINE | ID: mdl-31854129

ABSTRACT

Establishing healthy habits in the childcare setting for children aged 2-5 is pivotal for their healthy development and obesity prevention. The purpose of this qualitative study was to explore the perceptions and practices that childcare providers demonstrate when they promote healthy eating and physical activity among preschoolers. Four focus group interviews with 16 childcare providers were conducted in northwest Florida. A semistructured focus group guide was used. Discussions were audiorecorded and transcribed verbatim. Content analysis was performed to analyze the data with the use of ATLAS.ti software. This study followed the COREQ guidelines. Three major themes emerged from the data: (i) efforts to encourage children to eat healthfully and engage in physical activity; (ii) challenges in promoting healthy behaviors among children; and (iii) the need for more childcare provider training and parental involvement to encourage healthy eating. Regular childcare provider training and parental involvement are needed to establish a healthy environment for preschoolers.


Subject(s)
Child Care/methods , Health Promotion/methods , School Teachers/psychology , Child, Preschool , Florida , Focus Groups/methods , Health Knowledge, Attitudes, Practice , Health Promotion/standards , Humans , Male , Qualitative Research , School Teachers/standards , School Teachers/statistics & numerical data
8.
PLoS One ; 14(11): e0224557, 2019.
Article in English | MEDLINE | ID: mdl-31738802

ABSTRACT

The association between the social determinant of health (SDH) and sustainable development goals, has directed attention toward the influence of SDH. However, there is a lack of evidence regarding the instruments used to assess SDH. Thus, this study was conducted to assess the validity and reliability of the Korean Version of the Health Literacy on Social Determinants of Health Questionnaire (K-HL-SDHQ). A total of 660 workers in Korea participated in an online survey. The K-HL-SDHQ measures four dimensions (Access, Understand, Appraise, and Apply) with 33 items. The HL-SDHQ was translated into Korean using the forward-back translation method. To test the validity and reliability of the Korean translated HL-SDHQ, item analysis for the 33 items was conducted. Internal consistency was examined using Cronbach's α, an exploratory factor analysis, and a confirmatory factor analysis. The scale-level content validity index (S-CVI)/universal agreement of this study was .12 and S-CVI/average was .83 (item-CVI range = .50-1.00). The goodness of fit determined through a confirmatory factor analysis of the four dimensions was acceptable (χ2 (489) = 1475.054, p < .001, RMSEA = .06, CFI = .87, TLI = .85). The K-HL-SDHQ also demonstrated satisfactory internal consistency reliability (Cronbach's α = .92). The findings indicate that the K-HL-SDHQ is a valid and reliable tool that can be used to assess the SDH of workers in Korea. It is suggested that this tool can be applied through repeated research with workers and non-workers for health promotion, and to enhance researchers' understanding of the different levels of the HL-SDHQ.


Subject(s)
Health Literacy , Psychometrics/instrumentation , Social Determinants of Health , Translations , Adult , Female , Humans , Male , Middle Aged , Reproducibility of Results , Republic of Korea , Young Adult
9.
Res Nurs Health ; 42(5): 358-368, 2019 10.
Article in English | MEDLINE | ID: mdl-31410868

ABSTRACT

Most nurses in Korea work rotating shifts, an important contributor to fatigue. The Occupational Fatigue Exhaustion Recovery (OFER) Scale assesses work-related fatigue among nurses. In this study, we aimed to translate and culturally adapt the Korean version of this scale (OFER-K) with nurses working rotating shifts in Korea. Instrument adaptation was performed using committee-based translation, cognitive interviewing, and expert panel interviewing. Criterion validity, convergent validity, construct validity, internal consistency, and test-retest reliability were examined as psychometric properties of the OFER-K. An online survey was completed by 331 nurses; 107 of these nurses completed a second survey after 1 month to assess test-retest reliability. The overall Cronbach's alpha was 0.88. The correlation between participants' initial and retest responses for the total scale was 0.64 (p < .001). The chronic fatigue subscale was stable over time, t(106) = -1.76, p = .08. Criterion and convergent validity were supported by correlations between the OFER-K scale and the Chalder Fatigue Questionnaire, Epworth Sleepiness Scale, and Pittsburgh Sleep Quality Index. Confirmatory factor analysis showed a good fit using a three-factor model. The findings of this study showed that the OFER-K scale is a reliable and valid instrument for assessing chronic fatigue, acute fatigue, and inter-shift recovery in Korean nurses. Future research using this scale may lead to a better understanding of the antecedents and consequences of nurse fatigue and could provide important information to nurse researchers, administrators, and policymakers for developing interventions to reduce nurse fatigue.


Subject(s)
Burnout, Professional/diagnosis , Fatigue/diagnosis , Nursing Staff, Hospital/psychology , Nursing Staff, Hospital/statistics & numerical data , Adult , Cross-Sectional Studies , Female , Humans , Male , Psychometrics/methods , Reproducibility of Results , Republic of Korea , Surveys and Questionnaires , Translations
10.
J Pediatr Nurs ; 48: 55-62, 2019.
Article in English | MEDLINE | ID: mdl-31325800

ABSTRACT

PURPOSE: Pain assessment is the first step in managing pain; however, this can be challenging, particularly in settings such as the Pediatric Intensive Care Unit (PICU). This paper reports the current pain assessment practices from a study that was conducted describing the prevalence of pain, pain assessment, painful procedures, interventions, and characteristics of critically-ill children. Specifically, this paper addresses the child's communicative ability, pain scales, and characteristics of pain. DESIGN AND METHODS: The primary study was a cross-sectional, multi-site, descriptive design. Data from a 24-hour time period were collected from medical records and bedside nurses. RESULTS: Data were collected from the records of 220 children across 15 PICUs. The average number of pain assessments per child was 11.5 (SD 5.8, range 1-28). Seven behavioral scales and five self-report scales were used. There were times when no scale was used, "assume pain present" was recorded, or a sedation scale was documented. Twelve pain scales, including the target population, scoring, psychometric properties, and clinical utility are described. CONCLUSIONS: Results of this study indicate that a wide range of pain assessment tools are used, including behavioral scales for children unable to self-report. IMPLICATIONS: Foremost, the appropriate assessment method needs to be chosen for each child to manage pain. Knowledge of the criteria for the use of each pain assessment scale will help the clinician select the appropriate scale to use for each child. The practice of "assume pain present," as well as standardization of pain scales, and clinical support tools needs further investigation.


Subject(s)
Critical Care/organization & administration , Intensive Care Units, Pediatric/organization & administration , Pain Management/standards , Pain Measurement/standards , Pain/nursing , Child , Cross-Sectional Studies , Female , Humans , Pain/diagnosis , Pain Management/nursing , Pain Measurement/nursing , Surveys and Questionnaires
11.
J Nurs Manag ; 27(5): 884-895, 2019 Jul.
Article in English | MEDLINE | ID: mdl-30737987

ABSTRACT

AIM: To systematically evaluate the effect of work schedule characteristics on fatigue among shift nurses in hospital settings. BACKGROUND: The complexity and multidimensional nature of nursing work may lead to fatigue. This review mainly focused on work schedule characteristics that may mitigate the fatigue in nurses. EVALUATION: Six databases were searched, and eight relevant research articles published between 2000 and 2018 were identified. KEY ISSUES: The reviewed articles provided evidence supporting the association of work schedule characteristics such as total working hours, overtime, shift length and number of monthly night and evening shifts with fatigue. In addition, studies provided evidence for the positive association between insufficient rest period between shifts and fatigue among shift nurses in hospital settings. CONCLUSION: The reviewed studies provided mixed results regarding the associations between work schedule characteristics and nurse fatigue. However, quick returns and days called to work on days off were consistent factors contributing to nurse fatigue. More evidence is needed to arrive at a definitive conclusion about such relationships. IMPLICATION FOR NURSING MANAGEMENT: Nursing managers and administrators need to carefully review current rotating shift system and examine its impact on nurse fatigue as well as ensure enough resting time when developing nurse schedules.


Subject(s)
Fatigue/etiology , Shift Work Schedule/adverse effects , Fatigue/psychology , Hospitals/statistics & numerical data , Humans , Personnel Staffing and Scheduling/classification , Personnel Staffing and Scheduling/statistics & numerical data , Shift Work Schedule/psychology , Shift Work Schedule/standards , Work Schedule Tolerance/physiology , Work Schedule Tolerance/psychology
12.
Korean J Women Health Nurs ; 25(4): 423-433, 2019 Dec.
Article in English | MEDLINE | ID: mdl-37679912

ABSTRACT

PURPOSE: The study aimed to examine the effects of sleep pattern, stress, and attitude towards menstruation, and behavior that causes a reduction in exposure to endocrine disrupting chemicals (EDCs) in the course of premenstrual syndrome (PMS) in female adolescents. METHOD: In total, 200 girls attending middle school in K city were recruited via convenience sampling between December 11 and 31, 2018, and 192 subjects were selected for the analysis. Data were analyzed using descriptive statistics, an independent t-test, ANOVA, Pearson's correlation coefficient, and multiple regression. RESULTS: Overall, 9 (4.7%), 152 (79.2%), and 31 (16.1%) students had morning-, intermediate-, and evening-type sleep pattern, respectively. The mean scores for school-related stress were highest. Scores for stress regarding relationships with friends were the lowest. The mean score for attitudes toward menstruation was 72.33 out of 147. The mean score for behavior that led to a reduction in EDCs exposure was 56.57 out of 85. The mean scores for PMS severity were 25.30 and 38.39 out of 60. Analysis of factors affecting PMS revealed that severity of menstrual pain (ß=.28), use of analgesics during menstruation (ß=.17), family history of PMS (ß=-.16), stress (ß=.19), and behavior that reduces EDCs exposure (ß=-.17) exerted significant effects on PMS. The explanatory power of these variables was 37%. CONCLUSION: The findings propose the necessity of a health intervention program including educational information, which can reduce PMS in adolescents, induce the behaviors which can decrease exposure to EDCs, and activities for managing the stress levels.

13.
Jpn J Nurs Sci ; 16(2): 125-135, 2019 Apr.
Article in English | MEDLINE | ID: mdl-29888526

ABSTRACT

AIM: The purpose of this study was to evaluate the psychometric properties of the Korean-language versions of Pain Knowledge and Use (PKU-K), Collaboration and Satisfaction About Care Decisions (CSACD-K), and Environmental Complexity Scale (ECS-K). METHODS: A cross-sectional design was used with a convenience sample of 159 Korean nurses in seven neonatal intensive care units (NICUs). The data were collected by surveying the nurses with the PKU-K, CSACD-K, and ECS-K. Internal consistency reliability was assessed and Horn's parallel analysis, a confirmatory factor analysis, and a convergent construct validity test were conducted in order to evaluate the psychometric properties of the instruments. RESULTS: The PKU-K, CSACD-K, and ECS-K exhibited strong internal consistency reliability. Horn's parallel analysis showed four factor structures for the PKU-K, one for the CSACD-K, and three for the ECS-K. The confirmatory factor analysis showed a good model fit for the PKU-K and CSACD-K, but the ECS-K model showed a poor fit. Most factor loadings were statistically significant. The CSACD-K's convergent validity was supported by significant correlations for collegial nurse-physician relations with a validated instrument. CONCLUSION: The findings support the reliability and validity of the PKU-K, CSACD-K, and ECS-K for measuring nurses' knowledge about neonatal pain care, nurse-physician collaboration, and the work environment in NICUs. However, the ECS-K needs further refinement before it is applied to Korean NICU nurses.


Subject(s)
Nurses, Neonatal/psychology , Pain Management/methods , Adult , Cross-Sectional Studies , Factor Analysis, Statistical , Female , Humans , Intensive Care Units, Neonatal , Male , Middle Aged , Nursing Care , Psychometrics , Reproducibility of Results , Republic of Korea , Surveys and Questionnaires
14.
Healthc Inform Res ; 24(2): 97-108, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29770243

ABSTRACT

OBJECTIVES: This study aimed to identify and systematically review the literature on the use of mobile technology in nursing education. The research findings could evidence the effectiveness of mobile technology in undergraduate nursing students' learning outcomes. METHODS: Computerized searches were conducted using the Ovid-MEDLINE, Ovid-EMBASE, Cochrane Library, and CINAHL databases for relevant primary studies and limited to those between 2000 and February 2018. Only randomized controlled trials (RCTs) and quasi-experimental studies published in either English or Korean were included and critically appraised using Joanna Briggs Institute tools. RESULTS: Seven RCTs and 7 quasi-experimental studies were identified. The mobile device and intervention applied varied throughout all the studies. Studies published earlier in the 2000s found that immediate access to clinical and pharmacological referencing information through the mobile device increased students' efficacy in clinical practice. Later studies, which were mostly conducted in Korea, reported that smartphone-based applications could promote nursing students' learning motivation and satisfaction but not their clinical skills and knowledge. CONCLUSIONS: We still seem to be in the beginning stage of implementing mobile technology in nursing education due to the limited implication of mobile technology and inconsistent research conclusions. In the future, rigorous primary empirical studies are needed to suggest the effective use of mobile devices in nursing education.

15.
West J Nurs Res ; 40(2): 222-241, 2018 02.
Article in English | MEDLINE | ID: mdl-28322663

ABSTRACT

This study aimed to translate three English-language instruments-Pain Knowledge and Use (PKU), Collaboration and Satisfaction About Care Decisions (CSACD), and Environmental Complexity Scale (ECS)-into Korean and evaluate the equivalence of the instrument versions. Three Korean translators and two senior reviewers translated the instruments' 56 items using a committee approach. Eight Korean experts evaluated the cultural relevance of the translated instruments using a content validity index (CVI), and 12 Korean neonatal nurses were interviewed to assess their understanding of items. In an expert panel review, the item-CVI was less than 0.78 for 14 items. Based on cognitive interviews, four items were found to be unsuitable in Korea. Based on expert panel review and cognitive interviews, unsuitable items were modified or deleted. In another expert panel review, the scale-CVI was 1.00 for the final instruments. The findings support the validity of the Korean-language PKU, CSACD, and ECS for research application.


Subject(s)
Pain Management/nursing , Pediatrics/methods , Psychometrics/instrumentation , Psychometrics/standards , Adult , Female , Humans , Infant, Newborn , Intensive Care Units, Neonatal/organization & administration , Male , Reproducibility of Results , Republic of Korea , Surveys and Questionnaires , Translating , Workforce
16.
J Nurs Educ ; 56(6): 356-359, 2017 Jun 01.
Article in English | MEDLINE | ID: mdl-28585984

ABSTRACT

BACKGROUND: This study evaluated the reliability and validity of a new instrument, the Faculty Attitudes and Adoption of Simulation (FAAS). The FAAS was designed to identify faculty attitudes toward and levels of perceived knowledge and adoption of simulation. The instrument was developed based on a comprehensive literature review. METHOD: A convenience sample of 47 faculty members was used. Content validity, internal consistency reliability, and convergent construct validity were evaluated. RESULTS: Content validity, measured through a content validity index, was found to be .91. Pilot testing of the FAAS demonstrated a Cronbach's alpha of .83. Construct validity was evaluated by comparing the FAAS with a related tool called the Faculty Attitudes and Intent to Use Related to the Human Patient Simulator instrument (r = .64, p < .001). CONCLUSION: Pilot testing of the FAAS demonstrated initial reliability and validity for measuring faculty attitudes toward and perceived knowledge and adoption of simulation. [J Nurs Educ. 2017;56(6):356-359.].


Subject(s)
Education, Nursing/organization & administration , Faculty, Nursing/standards , Simulation Training/organization & administration , Surveys and Questionnaires/standards , Comprehension , Education, Nursing/standards , Educational Measurement , Female , Humans , Male , Pilot Projects , Psychometrics , Reproducibility of Results , Simulation Training/standards , Students, Nursing
17.
FEMS Microbiol Lett ; 363(18)2016 09.
Article in English | MEDLINE | ID: mdl-27535647

ABSTRACT

Glyoxal (GO) belongs to the reactive electrophilic species generated in vivo in all organisms. In order to identify targets of GO and their response mechanisms, we attempted to screen for GO-sensitive mutants by random insertions of TnphoA-132. The genes responsible for GO susceptibility were functionally classified as the following: (i) tRNA modification; trmE, gidA and truA, (ii) DNA repair; recA and recC, (iii) toxin-antitoxin; mqsA and (iv) redox metabolism; yqhD and caiC In addition, an insertion in the crp gene, encoding the cAMP responsive transcription factor, exhibits a GO-resistant phenotype, which is consistent with the phenotype of adenylate cyclase (cya) mutant showing GO resistance. This suggests that global regulation involving cAMP is operated in a stress response to GO. To further characterize the CRP-regulated genes directly associated with GO resistance, we created double mutants deficient in both crp and one of the candidate genes including yqhD, gloA and sodB The results indicate that these genes are negatively regulated by CRP as confirmed by real-time RT-PCR. We propose that tRNA as well as DNA are the targets of GO and that toxin/antitoxin, antioxidant and cAMP are involved in cellular response to GO.


Subject(s)
DNA Transposable Elements , Escherichia coli K12/drug effects , Glyoxal/pharmacology , Mutagenesis, Insertional , Adenylyl Cyclases/genetics , Cyclic AMP Receptor Protein/genetics , Drug Resistance, Bacterial/genetics , Escherichia coli K12/genetics , Escherichia coli Proteins/genetics , Glyoxal/metabolism , Mutation , RNA, Transfer/genetics
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