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1.
Anticancer Res ; 32(4): 1221-8, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22493352

ABSTRACT

Cytokeratin 20 (CK20) is an intermediate filament that is known to be a prognostic marker in several types of cancer. However, little is known about CK20 expression and tumor metastasis in tamoxifen-resistant MCF-7 (TRM-7) breast cancer cells. TRM-7 cells overexpress CK20, resulting in enhanced invasiveness in vitro. CK20 silencing reduced the invasiveness of TRM-7 cells. Moreover, CK20 expression in MCF-7 cells was regulated by peroxisome proliferator-activated receptor γ (PPARγ). Our findings suggest that PPARγ-dependent CK20 expression enhances the metastatic potential of MCF-7 breast cancer cells and may be a potential therapeutic target in tamoxifen-resistant breast cancer.


Subject(s)
Antineoplastic Agents, Hormonal/pharmacology , Keratin-20/metabolism , Neoplasm Invasiveness , Tamoxifen/pharmacology , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cell Line, Tumor , Drug Resistance, Neoplasm , Humans , Keratin-20/genetics , PPAR gamma/genetics , PPAR gamma/metabolism
2.
Exp Mol Med ; 43(5): 313-21, 2011 May 31.
Article in English | MEDLINE | ID: mdl-21499010

ABSTRACT

Persistently activated JAK/STAT3 signaling pathway plays a pivotal role in various human cancers including major carcinomas and hematologic tumors, and is implicated in cancer cell survival and proliferation. Therefore, inhibition of JAK/STAT3 signaling may be a clinical application in cancer therapy. Here, we report that 2-cyclohexylimino-6-methyl-6,7-dihydro-5H-benzo [1,3]oxathiol-4-one (BOT-4-one), a small molecule inhibitor of JAK/STAT3 signaling, induces apoptosis through inhibition of STAT3 activation. BOT-4-one suppressed cytokine (upd)-induced tyrosine phosphorylation and transcriptional activity of STAT92E, the sole Drosophila STAT homolog. Consequently, BOT-4-one significantly inhibited STAT3 tyrosine phosphorylation and expression of STAT3 downstream target gene SOCS3 in various human cancer cell lines, and its effect was more potent in JAK3-activated Hodgkin's lymphoma cell line than in JAK2-activated breast cancer and prostate cancer cell lines. In addition, BOT-4-one-treated Hodgkin's lymphoma cells showed decreased cell survival and proliferation by inducing apoptosis through down-regulation of STAT3 downstream target anti-apoptotic gene expression. These results suggest that BOT-4-one is a novel small molecule inhibitor of JAK3/STAT3 signaling and may have therapeutic potential in the treatment of human cancers harboring aberrant JAK3/STAT3 signaling, specifically Hodgkin's lymphoma.


Subject(s)
Antineoplastic Agents/pharmacology , Bridged Bicyclo Compounds, Heterocyclic/pharmacology , Enzyme Activation/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Janus Kinase 3/antagonists & inhibitors , Lymphoma/metabolism , STAT3 Transcription Factor/antagonists & inhibitors , Signal Transduction/drug effects , Animals , Antineoplastic Agents/chemistry , Apoptosis/drug effects , Bridged Bicyclo Compounds, Heterocyclic/chemistry , Cell Line , Cell Proliferation/drug effects , Cell Survival/drug effects , Drosophila/enzymology , Drosophila/metabolism , Drosophila Proteins/antagonists & inhibitors , Drosophila Proteins/metabolism , Humans , Janus Kinase 3/metabolism , Lymphoma/enzymology , Phosphorylation/drug effects , STAT Transcription Factors/antagonists & inhibitors , STAT Transcription Factors/metabolism , STAT3 Transcription Factor/metabolism
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