Localized pollution of veterinary antibiotics in watersheds receiving treated effluents from swine farms.

Swine excrement is discharged into surface waters mainly as effluent in Asian countries. As swine production consumes more antibiotics and less water than humans, a mismatch of the size of swine farms and that of the rivers receiving their effluent could create severe pollution by antibiotics. However, little is known about the occurrence of antibiotics in such rivers. We therefore monitored seven veterinary drugs, six human drugs (including a metabolite), three drugs for both use (including a metabolite), and major water qualities at 30 sites in Japanese watersheds where swine outnumber humans and where their excrement is largely treated on-site by aerobic biological wastewater processes. The compositions of veterinary drugs differed substantially among sites, unlike human drugs, indicating various patterns of use among swine farms. Median concentrations at the 30 sites were <1 ng/L for seven out of the ten drugs used in livestock, whereas maximum concentrations were >1000 ng/L for three and 100-1000 ng/L for four of them, giving median-maximum among the sites of >3 log for two and 2-3 log for six of them. The spatial distribution ranges of concentrations of veterinary drugs were wider than those of human drugs (mostly <1.5 log) and other analytes (mostly <1 log), despite the correlation between those of total veterinary drugs and nitrogen, attributable to fewer swine farms than households, the intensive animal husbandry, and the various drug-use patterns among the farms. The range of maximum concentrations of veterinary drugs in the watersheds was comparable to those reported in other Asian watersheds with less strict management of swine excrement, attributable to their slow decay in conventional wastewater treatment on swine farms. Thus, attention should be paid to hot-spot pollution of antibiotics on large Asian swine farms adjacent to streams with limited dilution capacity.

Oxygen delivery-guided perfusion for the prevention of acute kidney injury: A randomized controlled trial.

OBJECTIVES: The reduction of postoperative acute kidney injury in patients undergoing cardiopulmonary bypass surgery using an oxygen delivery-guided perfusion strategy (oxygen delivery strategy) for cardiopulmonary bypass management compared with a fixed flow perfusion (conventional strategy) remains controversial. The purpose of this study was to determine whether a oxygen delivery strategy would reduce the incidence of postoperative acute kidney injury in patients undergoing cardiopulmonary bypass surgery. METHODS: We randomly enrolled 300 patients undergoing cardiopulmonary bypass surgery. Patients were randomly assigned to a oxygen delivery strategy (maintaining a oxygen delivery index value >300 mL/min/m2 through pump flow adjustments during cardiopulmonary bypass) or a conventional strategy (a target pump flow was determined on the basis of the body surface area). The primary end point was the development of acute kidney injury. Secondary end points were the red blood cell transfusion rate and number of red blood cell units, intubation time, postoperative length of stay in the intensive care unit and the hospital, predischarge estimated glomerular filtration rate, and hospital mortality. RESULTS: Acute kidney injury occurred in 20 patients (14.6%) receiving the oxygen delivery strategy and in 42 patients (30.4%) receiving the conventional strategy (relative risk, 0.48; 95% confidence interval, 0.30-0.77; P = .002). The secondary end points were not significantly different between strategies. In a prespecified subgroup analysis of patients who had nadir hematocrit less than 23% or body surface area less than 1.40 m2, the oxygen delivery strategy seemed to be superior to the conventional strategy and the existence of quantitative interactions was suggested. CONCLUSIONS: An oxygen delivery strategy for cardiopulmonary bypass management was superior to a conventional strategy with respect to preventing the development of acute kidney injury.

Risk factors for postoperative delirium on oxygen delivery-guided perfusion.

BACKGROUND: Studies have demonstrated the efficacy of oxygen delivery-guided perfusion (ODGP) in preventing postoperative acute kidney injury, but the benefit of ODGP for delirium has not been confirmed. We retrospectively investigated the risk factors for postoperative delirium in patients who underwent ODGP (with oxygen delivery index [DO2i] > 300 mL/min/m2). METHODS: Consecutive patients who underwent on-pump cardiovascular surgery with ODGP from January 2018 to December 2020 were retrospectively analyzed. In addition to examining patients' DO2i during cardiopulmonary bypass (CPB), we quantified the two primary DO2 components-hematocrit (Hct) and pump flow. Delirium was defined based on the Intensive Care Delirium Screening Checklist (ICDSC). Patients were divided into three groups: no delirium (ICDSC score = 0), subsyndromal delirium (ICDSC score = 1-3), and clinical delirium (ICDSC score ≥ 4). RESULTS: Multivariate analysis identified only the number of red blood cell (RBC) units transfused, intubation time, and the cumulative time below the Hct threshold of 25% as predictive factors of postoperative delirium. Although patients with higher ICDSC scores had greater hemodilution during CPB, ODGP resulted in a higher pump flow, and DO2i was maintained above 300 mL/min/m2, with no significant difference between the three groups. CONCLUSIONS: A low Hct level during CPB with ODGP, the number of RBC units transfused, and intubation time were associated with postoperative delirium. Further investigations are needed to determine the ability of ODGP to prevent low Hct during CPB.

Long-Range Phase Order in Two Dimensions under Shear Flow.

We theoretically and numerically investigate a two-dimensional O(2) model where an order parameter is convected by shear flow. We show that a long-range phase order emerges in two dimensions as a result of anomalous suppression of phase fluctuations by the shear flow. Furthermore, we use the finite-size scaling theory to demonstrate that a phase transition to the long-range ordered state from the disordered state is second order. At a transition point far from equilibrium, the critical exponents turn out to be close to the mean-field value for equilibrium systems.

Rainbow Nambu-Goldstone Modes under a Shear Flow.

We study an O(N) scalar model under shear flow and its Nambu-Goldstone modes associated with spontaneous symmetry breaking O(N)→O(N-1). We find that the Nambu-Goldstone mode splits into an infinite number of gapless modes, which we call the rainbow Nambu-Goldstone modes. They have different group velocities and the fractional dispersion relation ω∼k_{1}^{2/3}, where k_{1} is the wave number along the flow. Such behaviors do not have counterparts in an equilibrium state.

Pustules on the back possibly triggering toxic-shock syndrome.

We herein describe an irregular case of toxic-shock syndrome (TSS). A previously healthy 28-year-old Japanese man developed a sudden-onset high fever. The patient was suffering from conjunctival hyperaemia, gastrointestinal symptoms such as vomiting and diarrhoea, and systemically diffused macular erythroderma. Further physical examination detected pustules on his back, which self-destructed over time. Laboratory revealed multiple organ failures. Subsequently, scalded skin on the face and desquamation in the limb extremities emerged by day 10, leading to the diagnosis of TSS, despite his stable circulatory dynamics through the course. Learning points for clinicians include that they should recall TSS as a possible disease concurrently causing high fever, systemic rash and multiple organ dysfunctions, even without being in a state of shock. The characteristic desquamations emerged in the limb extremities after hospitalisation were of help in diagnosing TSS.

Time-dose response of oxygen delivery during cardiopulmonary bypass predicts acute kidney injury.

OBJECTIVE: Previous studies have reported that nadir oxygen delivery during cardiopulmonary bypass is associated with the occurrence of postoperative acute kidney injury (AKI). However, these measurements only considered the bottom point of the oxygen delivery (DO2) but did not consider the duration of DO2. We aimed to examine whether the time-dose response of DO2 during cardiopulmonary bypass can be used to estimate the risk for postoperative AKI. METHODS: We evaluated 112 patients who underwent cardiac surgeries with cardiopulmonary bypass. We analyzed the perfusion parameters recorded every 20 seconds. To obtain time-dose response of DO2 index (DO2i), the area under the curve was calculated as below the 300 mL/min/m2 threshold, which accounts for depth and duration of cumulative oxygen debt. In addition, the cumulative time below DO2i 300 mL/min/m2 was also calculated. Receiver operating characteristic analysis, univariate regression analysis, and multivariate regression analysis were used to evaluate associations between perioperative variables and postoperative AKI. RESULTS: Patients who developed AKI had larger area under the curve below the 300 mL/min/m2 threshold (1581 vs 632; P < .01) and cumulative time below DO2i 300 mL/min/m2 (34.7 vs 15.3 minutes; P < .01). Nadir DO2i was not significantly different between the non-AKI and AKI groups (263.4 vs 247.0 mL/min/m2; P = .291). CONCLUSIONS: The time-dose response of DO2i during cardiopulmonary bypass is a better indicator than nadir DO2i in evaluating AKI risk. Maintaining DO2i levels >300 mL/min/m2 may result in decreased risk for postoperative AKI.

Virtual Torque Sensor for Low-Cost RC Servo Motors Based on Dynamic System Identification Utilizing Parametric Constraints.

We propose a novel virtual torque sensor for commercial low-cost radio-controlled (RC) servo motors. The virtual torque sensor has played an important role for conventional robots. It has been used for torque-required control applications such as human⁻robot interaction and under-actuated robots. However, most virtual torque sensors are based on the inversion of actuators or robot dynamics with the assumption that entire dynamics are known. This is not applicable to the RC servo motors that have unknown control structures. As RC servo motors enable researchers and hobbyists to create lightweight but high performance robots in an easy and cost-effective manner, the development of a virtual torque sensor for these motors is necessary. In this study, we propose a design method of a virtual torque sensor for RC servo motors. First, the virtual sensor is derived mathematically based on internal dynamic models with parametric constraints and compared to the conventional model. Second, a dedicated system identification method is developed for the proposed virtual sensor to implement the sensor in actual experiments. Finally, we compare experimental results with the measurements obtained by an actual sensor.

Relativistic hydrodynamics from the projection operator method.

We study relativistic hydrodynamics in the linear regime, based on Mori's projection operator method. In relativistic hydrodynamics, it is considered that an ambiguity about the fluid velocity occurs from the choice of a local rest frame: the Landau and Eckart frames. We find that the difference of the frames is not the choice of the local rest frame, but rather that of dynamic variables in the linear regime. We derive hydrodynamic equations in both frames by the projection operator method. We show that the natural derivation gives the linearized Landau equation. Also we find that, even for the Eckart frame, the slow dynamics is actually described by the dynamic variables for the Landau frame.

Effects of a 1.5 T time-varying magnetic field on cell volume regulation of bovine adrenal chromaffin cells in hyposmotic media.

Effects of a time-varying magnetic field on cell volume regulation by hyposmotic stress in cultured bovine adrenal chromaffin cells were examined. Through regulatory volume decrease (RVD), cell volume of chromaffin cells that were incubated in a hypotonic medium initially increased, reached a peak and finally recovered to the initial value. Two hour exposure to a magnetic field and addition of cytochalasin D increased peak value and delayed return to initial value. Intracellular F-actin contents initially decreased but returned to normal levels after 10 sec. Two hour exposure to the magnetic field and addition of cytochalasin D continuously reduced the F-actin content. Results suggest that exposure to the magnetic field stimulated disruption of the actin cytoskeleton and that the disruption delayed the recovery to the volume prior to osmotic stress.

Effects of exposure to a time-varying 1.5 T magnetic field on the neurotransmitter-activated increase in intracellular Ca(2+) in relation to actin fiber and mitochondrial functions in bovine adrenal chromaffin cells.

BACKGROUND: It has been reported that exposure to electromagnetic fields influences intracellular signal transduction. We studied the effects of exposure to a time-varying 1.5 T magnetic field on membrane properties, membrane cation transport and intracellular Ca(2+) mobilization in relation to signals. We also studied the mechanism of the effect of exposure to the magnetic field on intracellular Ca(2+) release from Ca(2+) stores in adrenal chromaffin cells. METHODS: We measured the physiological functions of ER, actin protein, and mitochondria with respect to a neurotransmitter-induced increase in Ca(2+) in chromaffin cells exposed to the time-varying 1.5 T magnetic field for 2h. RESULTS: Exposure to the magnetic field significantly reduced the increase in [Ca(2+)]i. The exposure depolarized the mitochondria membrane and lowered oxygen uptake, but did not reduce the intracellular ATP content. Magnetic field-exposure caused a morphological change in intracellular F-actin. F-actin in exposed cells seemed to be less dense than in control cells, but the decrease was smaller than that in cytochalasin D-treated cells. The increase in G-actin (i.e., the decrease in F-actin) due to exposure was recovered by jasplakinolide, but inhibition of Ca(2+) release by the exposure was unaffected. CONCLUSIONS AND GENERAL SIGNIFICANCE: These results suggest that the magnetic field-exposure influenced both the ER and mitochondria, but the inhibition of Ca(2+) release from ER was not due to mitochondria inhibition. The effect of eddy currents induced in the culture medium may indirectly influence intracellular actin and suppress the transient increase in [Ca(2+)]i.