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J Microbiol Methods ; 61(2): 201-8, 2005 May.
Article in English | MEDLINE | ID: mdl-15722146

ABSTRACT

Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.


Subject(s)
DNA Probes/genetics , Oligonucleotide Array Sequence Analysis/methods , Staphylococcus/classification , Animals , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , DNA Probes/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Humans , Polymerase Chain Reaction/methods , RNA, Ribosomal, 16S/chemistry , RNA, Ribosomal, 16S/genetics , Staphylococcus/genetics , Staphylococcus/isolation & purification , Superoxide Dismutase/chemistry , Superoxide Dismutase/genetics
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