Pseudosarcoma of the urinary bladder.

A case of pseudosarcoma of the urinary bladder is described in a woman aged 23. The tumor presenting with symptoms of cystitis arised in the left wall and invaded vesical cavity and pelvis. Histological, histochemical, immunohistochemical and electron microscope studies revealed a fibroblastic and myofibroblastic proliferation with atypias, accompanied by inflammatory infiltrates and rich vascularity. The tumor was furthermore characterized by extensive necrosis, involving the muscularis propria, and infiltrated the perivesical fat. The favourable outcome four years after partial cystectomy suggests the diagnosis of pseudosarcoma. The differential diagnosis and the pathogenesis of this rare lesion is discussed.

Primary vesical malignant lymphoma of histiocytes detected by urine cytology.

Urinary cytology performed in a woman aged 64 revealed an uncommon cell population with malignant features highly suggestive of a lymphoma. Histologic and immunohistochemical investigations of a polypoid endovesical mass surgically resected showed a lymphoma of histiocytes in the lamina propria of the bladder wall. Clinical, operative and pathologic staging demonstrated that the tumor was confined to bladder. The case repeats the typical features of primary vesical lymphoma reported in the literature. The favourable follow up six years after surgery confirms the good prognosis of bladder Iymphoma treated by aggressive therapy.

Lack of detection of p53 expression in retinoblastoma tumor cells.

p53 was examined by immunohistochemistry in five cases of retinoblastoma, a neoplasm that is caused by a loss of function of the Retinoblastoma susceptibility gene (Rb), mapped to chromosome 13q14. Object of the study was the identification of eventual further gene mutation in retinoblastoma tumor cells determining the onset of an independent tumoral monoclonal cell subset. We did not observe any positive reaction for p53 protein expression in the five cases we analyzed.

p53 protein overexpression in a case of conjunctival micro-invasive carcinoma.

We analysed by immunohistochemistry the expression of p53 protein in a case of micro-invasive carcinoma of the conjunctiva. About 50% of tumor cells showed a strong nuclear positivity for p53. This suggests that p53 gene alterations play a role in the development of this type of tumor.

HPV and p53 in urinary bladder carcinoma.

We report a study on human papilloma virus (HPV) and p53 protein in 18 cases of urothelial carcinoma grade 1, 2 and 3. The presence of HPV has been correlated to the p53 protein expression, as this virus, once integrated in the cell nuclei, seems to cause the alteration of some genes expression, involved in the cell-cycle regulation, like p53. One case of urothelial papillary carcinoma grade 2, infiltrating the lamina propria, resulted to be positive for HPV type 31/33/51 and for p53 protein. Our data suggest that HPV type 31/33/51 may have played a role in the pathogenesis of this neoplasia causing an alteration of p53 gene.

Bilateral leiomyoma of the epididymis.

The rare event of a bilateral leiomyoma of the epididymis is illustrated. The tumors were located near the tail of the epididymis in a man aged 58, which furthermore presented hydrocele and had been previously operated because of bilateral inguinal hernia. The differentiation with inflammatory pseudotumor, leiomyosarcoma and adenomatoid leiomyoma is discussed.

Detection of human papillomavirus (HPV) DNA type 6/11 in a conjunctival papilloma by in situ hybridization with biotinylated probes.

Four cases of conjunctival papilloma in two different patients were examined by in situ hybridization for HPV DNA type 6/11, 16/18 and 31/33/51. Formalin-fixed and paraffin-embedded tissues were hybridized by biotinylated probes. One tumor and one of its recurrences showed nuclear positivity for HPV 6/11 in the superficial cells of the epithelium. The results suggest that HPV type 6/11 may be etiologic agent of conjunctival papillomas. The benign behavior of these neoplasms may be related to the etiologic role of this type of HPV.

Cytological features of a craniopharyngioma.

The fine needle aspiration cytology of a cystic suprasellar lesion, which caused bilateral hemianopia in a woman aged 63, resulted to be consistent with a craniopharyngioma and was confirmed by tissue diagnosis. The cytological pattern showed macrophages and debris with calcifications indicating the presence of a cyst. Furthermore squamous cells of basal type and ghost cells were identified. These findings together with the seat of the lesion allowed for the diagnosis of craniopharyngioma and for the differentiation from other cystic lesions of the same region. The case confirms the value of the fine needle aspiration cytology in the detection of craniopharyngioma and points out cytological peculiarities of this not rare neoplasm.

Carotid body paraganglioma (chemodectoma): cytologic remarks.

The fine needle aspiration findings of a carotid body paraganglioma observed in a female patient aged 18 showed a pattern similar to that of previously described cases, but also some other morphological features to be noticed: a continuous spectrum of nuclear volumetric variations, rosette-like structures, cell embracing phenomenon and characteristics nuclear clear areas. We believe that these findings may help in the cytological distinction of carotid body paraganglioma from other laterocervical neoplasms.

Paraurethral leiomyoma in women.

Two rare cases of paraurethral leiomyoma in women are presented. Physical examination and cystourethrography with previous cytourethroscopy revealed the seat of the masses. Surgical approach by means of incision of the anterior vaginal wall allowed for the complete enucleation of the tumors.

Fine needle aspiration cytology of parathyroid adenoma. A review of seven cases.

The value of fine needle aspiration (FNA) cytology in the diagnosis of parathyroid adenomas was demonstrated by a cytohistologic review of seven cases. The cytologic patterns, which were characterized by numerous, mostly isolated epithelial cells and naked nuclei showing anisokaryosis and multiple nucleoli, were consistent with the histologic findings in this neoplasm, which should be considered in the differential (FNA) diagnosis of masses in the neck region. The only diagnostic problem is its differentiation from follicular thyroid neoplasms.

Grade 2 urethroprostatic papillary urothelial carcinoma with positive urinary cytology.

A rare case of papillary urothelial grade 2 carcinoma of the prostate and urethra in a patient with a previous papillary neoplasm of the bladder is reported. The tumor caused hematuria and prostatic enlargement suspicious for cancer. Urinary cytology was diagnostic.

[Detection of a malignant abdominal lymphoma by urine cytology]. / Entdeckung eines malignen abdominellen Lymphoms durch Urinzytologie.

Urine cytology performed in a 64 year old man with pain in the left flank and hematuria revealed tumor cells suggesting a malignant lymphoma. Subsequent radiologic and operative findings showed a neoplasm in the left renal region involving intraabdominal and left inguinal lymph nodes. Biopsies from lymph nodes confirmed the presence of a malignant lymphoma probably of histiocytic type. The histologic classification of the tumor is discussed and the rarity of the finding of lymphoma cells in the urine is noted.