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1.
Sci Total Environ ; 917: 169306, 2024 Mar 20.
Article in English | MEDLINE | ID: mdl-38103614

ABSTRACT

Microcystins (MCs) are the most common cyanobacterial toxins. Epidemiological investigation showed that exposure to MCs can cause gastro-intestinal symptoms, gastroenteritis and gastric cancer. MCs can also accumulate in and cause histopathological damage to stomach. However, the exact mechanisms by which MCs cause gastric injury were unclear. In this study, Wistar rats were administrated 50, 75 or 100 µg microcystin-LR (MC-LR)/kg, body mass (bm) via tail vein, and histopathology, response of anti-oxidant system and the proteome of gastric tissues at 24 h after exposure were studied. Bleeding of fore-stomach and gastric corpus, inflammation and necrosis in gastric corpus and exfoliation of mucosal epithelial cells in gastric antrum were observed following acute MC-LR exposure. Compared with controls, activities of superoxide dismutase (SOD) were significantly greater in gastric tissues of exposed rats, while activities of catalase (CAT) were less in rats administrated 50 µg MC-LR/kg, bm, and concentrations of glutathione (GSH) and malondialdehyde (MDA) were greater in rats administrated 75 or 100 µg MC-LR/kg, bm. These results indicated that MC-LR could disrupt the anti-oxidant system and cause oxidative stress. The proteomic results revealed that MC-LR could affect expressions of proteins related to cytoskeleton, immune system, gastric functions, and some signaling pathways, including platelet activation, complement and coagulation cascades, and ferroptosis. Quantitative real-time PCR (qRT-PCR) analysis showed that transcriptions of genes for ferroptosis and gastric function were altered, which confirmed results of proteomics. Overall, this study illustrated that MC-LR could induce gastric dysfunction, and ferroptosis might be involved in MC-LR-induced gastric injury. This study provided novel insights into mechanisms of digestive diseases induced by MCs.


Subject(s)
Antioxidants , Marine Toxins , Microcystins , Rats , Animals , Antioxidants/metabolism , Microcystins/toxicity , Microcystins/metabolism , Proteomics , Liver/metabolism , Rats, Wistar , Oxidative Stress , Glutathione/metabolism , Stomach
2.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-873228

ABSTRACT

Objective:To discuss clinical effect of addition and subtraction therapy of Wuhutang combined with Qingjin Jianghuotang to community acquired pneumonia (CAP) in children with syndrome of phlegm heat closing lung, and to study the influence to inflammatory factors. Method:One hundred and forty patients were randomly divided into control group (69 cases) and observation group (71 cases) by random number table. Patients in two group of chidren got comprehensive symptomatic treatment measures of anti-infection, antipyretic, expectorant, antiasthmatic and respiratory support of inflammatory factors. The control group was treated with Lingyang Qingfei granules.1 g/time,3 time/day. Patients in observation group added addition and subtraction therapy of Wuhutang combined with Qingjin Jianghuotang, 1 dose/day. The courses of treatment in two groups were 7 days. And temperature, time of antipyretic, time of complete antipyretic and rate of complete antipyretic at the 7th day after treatment were recorded. And release time and disappearance time of cough, expectoration, disappearance time of pulmonary rales and treatment failure were also recorded. And before and after treatment, scores of syndrome of phlegm heat closing lung were graded, and levels of serum high sensitive C-reactive protein (hs-CRP), procalcitonin (PCT), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected. Result:Analyzed by rank sum test, effect in observation group was better than that in control group (Z=2.106, P<0.05), and curative effect of traditional Chinese medicine (TCM) syndrome was also better than that in control group (Z=2.119, P<0.05). Time of antipyretic, time of complete antipyretic, release time and disappearance time of cough and expectoration and disappearance time of pulmonary rales were all shorter than those in control group (P<0.01). Rate of complete antipyretic at the 7th day after treatment in observation group was 96.92%(63/65) higher than 82.81%(53/64) in control group (χ2=7.085, P<0.05). Failure rate of treatment was 9.23%(6/65) lower than 23.44%(15/64) in control group (χ2=4.775, P<0.05). And major symptom, physical sign score, minor symptom score, the total score of syndrome of phlegm heat closing lung and levels of hs-CRP, PCT, TNF-α and IL-6 were all lower than those in control group (P<0.01). Conclusion:On the basis of comprehensive anti-infection treatment, addition and subtraction therapy of Wuhutang combined with Qingjin Jianghuotang can control the clinical symptoms, and the advantages of rapid onset, rapid symptom regression, short course of disease can be found, and it can also reduce the inflammatory reaction, control the progress of the disease. The complete antipyretic rate, disease efficacy and TCM syndrome efficacy are better.

3.
Journal of Forensic Medicine ; (6): 299-304, 2020.
Article in English | WPRIM (Western Pacific) | ID: wpr-985116

ABSTRACT

Objective To provide reference indexes and theoretical basis for age estimation of blood pools by investigating the entire drying process and monitoring the change of morphology and mass. Methods Four 15 mL blood pool samples were prepared on the clean ceramic plate. The change of morphology and mass of blood pools in a closed dark environment with a temperature of (20.0±0.5) ℃ and a humidity of 35%-45% were dynamically observed from 0 h to 60 h. Images of the blood pools were recorded by digital camera. The area of blood pools was calculated by MATLAB R2014b, the length of cracks was measured by Image J and the statistical analysis was performed by SPSS 16.0. Results By summarizing and analyzing, the drying of blood pools was divided into five stages: coagulation (0-4.5 h), gelation (>4.5-20.0 h), gel-solid mixing (>20.0-37.0 h), solid (>37.0-40.0 h) and final desiccation (>40.0-45.0 h). From 0 to 45 h, the mass of the blood pools decreased linearly with time, and the decrease was not obvious from 45.0 to 60.0 h. The standardized mass (y2) showed strong correlation with the time (x) y2=0.018 2 x+0.271 4(R2=0.967 9). The area change rate of blood pools, the distance that the edge of blood pools moved, the average length of radical cracks had little correlation with the time that passed. Conclusion The overall morphological characteristics of blood pools show a certain regularity with the time and the standardized indexes established provide a reference for the age estimation of blood pools.


Subject(s)
Blood Coagulation , Humidity , Temperature , Time Factors
4.
Neurology Asia ; : 49-58, 2017.
Article in English | WPRIM (Western Pacific) | ID: wpr-625433

ABSTRACT

Background & Objective: Radiotherapy and temozolomide are the standard therapy for newly diagnosed glioblastoma multiforme (GBM). However, it is unclear whether adding another agent to the commonly used radiotherapy-temozolomide (RT + TMZ) benefits newly diagnosed GBM patients. The present network meta-analysis aimed to assess the efficacy of combining other agents with RT + TMZ for GBM treatment. Methods: A comprehensive literature search was conducted on PubMed, EMBASE.com, Web of Science, and the Cochrane Central Register of Controlled Trials from inception to September 23, 2014, to include all randomized controlled trials of RT + TMZ-based therapy in GBM patients. Pairwise and network meta-analyses were performed to compare the therapeutic regimens. Results: Seventeen studies involving 4,148 patients were identified. The results of pairwise meta-analysis indicated no significant differences among most comparison groups, except for bevacizumab + RT + TMZ versus RT + TMZ for progression-free survival (hazard ratio [HR] = 0.71, 95% confidence interval [CI]: 0.59–0.86; P = 0.000) and RT + TMZ versus RT alone for overall survival (HR = 0.71, 95% CI: 0.58–0.88; P = 0.001). The results of network meta-analysis also showed no significant differences in most comparisons; however, adverse events were more common among patients receiving additional therapeutic agents other than RT + TMZ. The ranking probability analysis indicated that bevacizumab + RT + TMZ and nimustine + cisplatin + RT + TMZ were associated with the best progression-free and overall survival, but they also caused the most adverse events in GBM patients. RT + bevacizumab + irinotecan had the highest probability of being the best regimen for minimizing adverse events. Conclusions: The addition of other targeted agents, particularly bevacizumab and nimustine, to RT + TMZ could be slightly effective for the treatment of newly diagnosed GBM patients; however, adverse events remained common.


Subject(s)
Glioblastoma
5.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-491457

ABSTRACT

Objective To investigate the effect and mechanism of liver X receptor ( LXR ) agonist on expression of fatty acid synthase( FAS) in diabetic kidney. Methods In the part of in vivo study, immunostaining was used to detect the FAS protein expression in kidney. 16-week-old male db/db mice on C57BL/6 background were administered via gavage a LXR synthetic agonist, TO901317, at a dose of 3 mg · kg-1 · d-1 or vehicle ( 0. 5%Carboxymethyl Cellulose Sodium, CMC-Na) alone for 7 d;Quantitative RT-PCR and Western blot were used to detect mRNA and protein levels of FAS and SREBP-1. In the part of in vitro study, MCT cell(a mouse murine proximal tubule cell line)was treated with 10μmol/L TO901317 for 24 h or transfected with active SREBP-1c expression vector (SREBP-1cN). HEK293 cells(a human renal tubule cell line)were transfected with mFAS-(1. 7 kb)-luc, LXR expression vector or SREBP-1cN for 12 h. Quantitative RT-PCR and luciferase reproter assay were utilized to examine FAS mRNA level and FAS promoter activity. Results FAS was abundantly expressed in renal cortex, with low expresson in renal glomeruli. The mRNA and protein expressious of FAS in kidney of db/db mice were lowered compared with db/m mice. TO90137 treatment increased FAS mRNA expression by 1. 3-fold. TO901317 increased expression of SREBP-1 in kidneys of db/m and db/db mice by 5. 1-fold and 17-fold, respectively. TO901317 and overexpression of SREBP-1c increased expression of FAS in MCT cells by 1. 5-fold and 1. 8-fold. Transcription activity of FAS were induced by TO901317, LXR, and SREBP-1cN overexpressions in HEK293 cells. Conclusions Both direct(LXRE)and indirect(SREBP-1c)mechanisms may contribute to the up-regulation of FAS expression by LXR in renal proximal tubule cells.

6.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-294393

ABSTRACT

<p><b>OBJECTIVE</b>To explore the clinical roles of Jiawei Shentong Zhuyu Decoction (JSZD) in preventing the occurrence of failed back surgery syndrome (FBSS), and to observe its effect on serum tumor necrosis factor-alpha (TNF-alpha).</p><p><b>METHODS</b>Totally 100 patients prepared for surgical operation due to lumbar intervertebral disc herniation were randomly assigned to the treatment group and the control group according to random number table, 50 cases in each group. Patients in the treatment group additionally took JSZD, one dose per day, taken in two portions, once in the morning and once in the evening. Those in the control group took Celecoxib Capsule (200 mg each time, once per day) and Mecobalamin Tablet (0.5 mg each time, 3 times per day). They only took Mecobalamin Tablet from the 11th day. All patients were treated for 30 days. Japanese Orthopaedic Association (JOA) score was performed before treatment, at week 1, after treatment, at 6 months of followed-ups, and at 12 months of followed-ups. And the levels of TNF-alpha in the peripheral blood were observed before treatment and at one month after treatment.</p><p><b>RESULTS</b>Totally 93 patients completed the followed-up study. The JOA scores were improved after treatment, at 6 and 12 months of followed-ups (P < 0.05, P < 0.01). The JOA score at 6 months of followed-ups was superior in the treatment group to that of the control group (P < 0.05). Five patients (accounting for 10.6%) suffered from FBSS in the treatment group, while 9 (accounting for 19.6%) suffered from FBSS in the control group. The treatment group was superior to the control group (P < 0.05). The TNFalpha level was improved after treatment in the two groups. Of them, the improvement of TNF-alpha in the treatment group was better than that of the control group (P < 0.05).</p><p><b>CONCLUSION</b>The application of JSZD was effective for preventing the occurrence of FBSS, and improved the serum TNF-alpha level.</p>


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Drugs, Chinese Herbal , Therapeutic Uses , Failed Back Surgery Syndrome , Intervertebral Disc Displacement , Lumbar Vertebrae , General Surgery , Tumor Necrosis Factor-alpha , Blood
7.
Int Wound J ; 10(5): 562-72, 2013 Oct.
Article in English | MEDLINE | ID: mdl-22682212

ABSTRACT

This study aims to explore the influence of hyaluronic acid (HA) on wound healing during xenogeneic porcine acellular dermal matrix (PADM) composite skin grafting. The results will facilitate the development of methods for improving graft contracture and poor elasticity of composite transplantation. Exogenous HA was added to composite PADM grafts and to thin autologous skin grafts during rabbit full-thickness skin wound repair. The influence of HA on wound healing was evaluated according to its contracture rate and its expression of collagen types I and III. The possible mechanism was then explored based on HA metabolism and vascularisation in the skin graft. The results show that exogenous HA relieves graft contracture on rabbit wound surfaces, increases collagen I and III expression and decreases the ratio between collagen types. HA stimulates the generation of more CD44 receptors to strengthen its enzymolysis. The resulting metabolites promote the vascularisation of the wound surface, which are conducive for mitigating graft contracture, and further improve the composite grafting effect.


Subject(s)
Acellular Dermis , Hyaluronic Acid/pharmacology , Skin Transplantation/methods , Skin/injuries , Wound Healing/drug effects , Wounds and Injuries/surgery , Animals , Autografts , Collagen Type I/metabolism , Collagen Type III/metabolism , Disease Models, Animal , Female , Male , Rabbits , Skin/metabolism , Treatment Outcome
8.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 29(6): 431-4, 2013 Nov.
Article in Chinese | MEDLINE | ID: mdl-24624881

ABSTRACT

OBJECTIVE: To explore the clinical application of mandibular-driven simultaneous maxillo-mandihular distraction to correct hemifacial microsomia with rapid prototyping technology. METHODS: The patient' s skull resin model was manufactured with rapid prototyping technology. The osteotomy was designed on skull resin model. According to the preoperative design, the patients underwent Le Fort I osteotomy and mandibular ramus osteotomy. The internal mandible distractor was embedded onto the osteotomy position. The occlusal titanium pin was implanted. Distraction were carried out by mandibular-driven simultaneous maxillo-mandihular distraction 5 days after operation. RESULTS: The distraction in five patients was complete as designed. No infection and dysosteogenesis happened. The longest distance of distraction was 28 mm, and the shortest distance was 16 mm. The facial asymmetry deformity was significantly improved at the end of distraction. The ocelusal plane of patients obviously improved. CONCLUSIONS: Rapid prototyping technology is helpful to design precisely osteotomy before operation. Mandibular-driven simultaneous maxillo-mandibular distraction can correct hemifacial microsomia. It is worth to clinical application.


Subject(s)
Face/abnormalities , Facial Asymmetry/congenital , Goldenhar Syndrome/surgery , Hyperplasia/surgery , Mandible/surgery , Maxilla/surgery , Osteogenesis, Distraction/methods , Osteotomy/methods , Face/surgery , Facial Asymmetry/surgery , Humans , Osteotomy, Le Fort
9.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-359253

ABSTRACT

<p><b>OBJECTIVE</b>To explore the clinical efficacy of open-door cervical laminoplasty combined Modified Buyang Huanwu Decoction (MBHD) to treat cervical spinal canal stenosis (CSCS).</p><p><b>METHODS</b>Totally 32 CSCS patients were randomly assigned to two groups, Group A (17 cases, treated by laminoplasty) and Group B (15 cases, treated by laminoplasty combined MBHD). All patients received open-door cervical laminoplasty. Those in Group B took MBHD additionally for 2 weeks after surgery. The visual analogue scale (VAS), the Japanese Orthopedic Association (JOA) score, and the neck disability index (NDI) were measured preoperative, postoperative 3 months and 12 months, respectively.</p><p><b>RESULTS</b>There was no statistical difference in preoperative VAS, JOA, or NDI (P > 0.05). The VAS, JOA, and NDI were obviously improved 3 months and 12 months after surgery in the two groups, showing statistical difference when compared with before surgery in the same group (P < 0.01). At 3 months after surgery the aforesaid indices in Group B were superior to those in Group A (P < 0.05). There was no statistical difference in the aforesaid indices between the two groups at 12 months after surgery (P > 0.05).</p><p><b>CONCLUSION</b>MBHD favorably improved early recovery of neural functions of CSCS patients (3 months after surgery).</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Cervical Vertebrae , Drugs, Chinese Herbal , Therapeutic Uses , Orthopedic Procedures , Methods , Phytotherapy , Spinal Stenosis , Therapeutics , Treatment Outcome
10.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-343495

ABSTRACT

<p><b>OBJECTIVE</b>To explore the clinical application of mandibular-driven simultaneous maxillo-mandihular distraction to correct hemifacial microsomia with rapid prototyping technology.</p><p><b>METHODS</b>The patient' s skull resin model was manufactured with rapid prototyping technology. The osteotomy was designed on skull resin model. According to the preoperative design, the patients underwent Le Fort I osteotomy and mandibular ramus osteotomy. The internal mandible distractor was embedded onto the osteotomy position. The occlusal titanium pin was implanted. Distraction were carried out by mandibular-driven simultaneous maxillo-mandihular distraction 5 days after operation.</p><p><b>RESULTS</b>The distraction in five patients was complete as designed. No infection and dysosteogenesis happened. The longest distance of distraction was 28 mm, and the shortest distance was 16 mm. The facial asymmetry deformity was significantly improved at the end of distraction. The ocelusal plane of patients obviously improved.</p><p><b>CONCLUSIONS</b>Rapid prototyping technology is helpful to design precisely osteotomy before operation. Mandibular-driven simultaneous maxillo-mandibular distraction can correct hemifacial microsomia. It is worth to clinical application.</p>


Subject(s)
Humans , Face , Congenital Abnormalities , General Surgery , Facial Asymmetry , General Surgery , Goldenhar Syndrome , General Surgery , Hyperplasia , General Surgery , Mandible , General Surgery , Maxilla , General Surgery , Osteogenesis, Distraction , Methods , Osteotomy , Methods , Osteotomy, Le Fort
11.
Zhonghua Yi Xue Za Zhi ; 92(24): 1719-22, 2012 Jun 26.
Article in Chinese | MEDLINE | ID: mdl-22944167

ABSTRACT

OBJECTIVE: To explore the effects of hyaluronic acid (HA) on biomechanical properties for porcine acellular dermal matrix (PADM) plus thin skin autograft after transplantation. METHODS: The dorsa of 10 Japanese white rabbits were symmetrically divided into four areas of A-D by random grouping. Full-thickness skin defects were created in Groups A-C while Group D was blank with normal skin. Operations were performed in Group A: implant with HA + PADM + thin skin autografts, Group B: implant with PADM + thin skin autografts and Group C: skin autografts group. Histological examination of specimen was performed at Day 56 postoperatively. And the biomechanical properties such as relaxation and stress-strain properties of grafts were recorded. RESULTS: The structure of PADM was found to be basically intact by hematoxylin and eosin E dyeing in Groups A and B. In Group A, dense fiber structure could be observed. Lots of regularly arranged collagenous fibers and new blood capillaries were grown into the dermal matrix with sparsely distributed inflammatory cells. In Group B, acellular dermal matrix became clustered with a small amount of invaded fibroblasts. And there was a high expression of inflammatory cells. The biomechanic performances of transplanted skin were: Group A's curve was mostly close to that of Group D's, Group B's curve was the most further from that of Group D's (P = 0.001) and Group C's curve stayed between Groups A and B. Under the same strain, the stress of Groups A-D was (87 ± 8), (115 ± 9), (63 ± 7) and (81 ± 4) kPa respectively. No significant difference of stress existed between these two groups (P = 0.838). There was significant difference of stress between Groups B/C and D (P = 0.001 and P = 0.009). CONCLUSION: Topical hyaluronic acid may be used to enhance the biomechanics performances of transplanted skin.


Subject(s)
Acellular Dermis , Hyaluronic Acid/pharmacology , Skin Transplantation , Animals , Biomechanical Phenomena , Rabbits , Swine , Transplantation, Autologous , Transplantation, Heterologous
12.
Zhonghua Yi Xue Za Zhi ; 92(20): 1428-30, 2012 May 29.
Article in Chinese | MEDLINE | ID: mdl-22883205

ABSTRACT

OBJECTIVE: To explore the effect on biomechanical compliance after an addition of hyaluronic acid (HA) in transplantation of porcine acellular dermal matrix (PADM) with skin autograft. METHODS: Ten Japanese white rabbits were used as experimental animal, the dorsa of rabbits was symmetrically divided into four area, and then grouping was randomized. Full-thickness skin defects were created in Group A to Group C, and Group D was blank group with normal skin. The following procedures were performed: Group A: implanted with HA+PADM+ thin skin autografts, GroupB: implanted with PADM+ thin skin autografts and Group C: skin autografts, except for Group D. The wound tissue specimens were harvested at 70 days postoperatively, and they were recorded contracture rates of the grafts and the biomechanical compliance by Instron biomechanics tensiometer. The expression of collagens I and III in dermal fibroblasts of each group was determined by Western blot. RESULTS: On postoperation day 70, the grafts contracture rates in Group A to Group C were 10.2% ± 0.6%, 36.6% ± 0.8%, 32.7% ± 1.4% respectively, there were notable significant difference among three groups (P = 0.000). Under the same tension, there was no significant difference in strain between Groups A and D ((83 ± 8) vs (81 ± 5) kPa, P = 0.552). Some significant difference in strain existed between Groups B and D ((215 ± 9) vs (81 ± 5) kPa, P = 0.000). The strain of Group C ((106 ± 7) kPa) was between Groups B and D, and significant strain difference existed among them (P = 0.000). The expressions of collagens I and III in Group A were higher than those in Group B, while the ratio of collagens I to III in Group A was lower than that in Group B. CONCLUSION: Topical HA may be used to increase the expressions of type I and III collagen, decrease the ratio of collagen type I/III, and enhance the biomechanics performances of transplanted skin.


Subject(s)
Acellular Dermis , Hyaluronic Acid/pharmacology , Skin Transplantation , Skin/drug effects , Animals , Collagen Type I/metabolism , Collagen Type III/metabolism , Compliance , Rabbits , Swine , Transplantation, Autologous , Transplantation, Heterologous
13.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-635557

ABSTRACT

BackgroundResearches found that the posterior capsular opacification (PCO) after lensextraction is associated with the elevation of the transforming growth factor-β(TGF-β).To seek the drug for inhibitingproliferation of lens epithelial cells (LECs) is crucial in the treatment and prevention of PCO.ObjectiveThisstudy was to investigate the preventing effects of decorin on the proliferation of LECs.MethodsRabbit LECs wascultured and passaged.The LECs in growth phase were incubated in 96 well plate at the density of 8×106/L.Decorinwith the concentrations 0.1,1.0,10.0 mg/L was added into the medium for 24,48 and 72 hours respectively.0.1%DMSO was used at the same way as positive control group,and the regular cultured cells worked as blank controlgroup.The inhibitory rates of different concentrations of decorin on the growth of LECs were detected by MTT at 24,48and 72 hours after addition of decorin.The percentage of LECs in different cell cycles in various groups was assayedusing flow cytometry.TGF-β level in medium suspension was detected using ELISA.The expression of TGF-β mRNA in LECs was checked by RT-PCR,and α-SMA expression in LECs was determined using immunochemistry.Results ELISA assay showed a statistical difference in the TGF-β levels of different groups (F=39.24,P=0.03 ).The TGF-β levels in 1.0,10.0 mg/L decorin groups were significantly decreased in comparison with blank control group (P<0.01) and 0.1 mg/L decorin group (P<0.05 ).The inhibitory rates of decorin in the concentrations of ≥ 1.0 mg/L on the growth of LECs were higher than the blank control group,and those in various concentrations of decorin groups were considerably lower in 24 hours compared with 48 and 72 hours ( P<0.05 ) and so was the 48 hours compared with 72 hours (P<0.05 ).The percentages of LECs in G0/G1 phase were ascent in 0.1,1.0 and 10.0 mg/L decorin groups in comparison with G2/M and S phase (P<0.05).Immunochemistry revealed the weak expression of α-SMA in various decorin groups in comparison with control group. Conclusions Decorin can effectively inhibit LECs growth and induce LECs apoptosis in concentration- and time-dependent manner.It is suggested that decorin can be used in the prevention and treatment of after cataract.

14.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-321850

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effects of surgical treatment of IsoC-3D navigation assisted percutaneous pedicle screw fixation,vertebral canal decompression and interbody fusion using Mast Quadrant system in patients with lumbar degenerative disease.</p><p><b>METHODS</b>From January 2009 to February 2010,21 patients with lumbar degenerative disease were treated with IsoC-3D navigation under Mast Quadrant system. There were 12 males and 9 females, with an average age of 50.2 years (ranged,36 to 72 years). All patients underwent discectomy,vertebral canal decompression, cage implantation using Mast Quadrant system and IsoC-3D navigation assisted sextant lumbar fixation. Clinical outcomes were evaluated with Oswestry disability index (ODI), Visual analog scale (VAS) and degree of satisfaction of patients.</p><p><b>RESULTS</b>Eighteen patients (85.7%) were followed up from 6 to 18 months with an average of 10 months. No surgery-related complications were found. The preoperative, postoperative ODI scores was 49.6 +/- 12.2 and 17.2 +/- 9.2, respectively (P < 0.01); VAS score of leg pain decreased from preoperative 75.2 +/- 10.0 to 12.2 +/- 11.8 at final follow-up (P < 0.01); VAS score of lumbago decreased from preoperative 59.9 +/- 17.3 to 16.6 +/- 11.5 at final follow-up (P < 0.01). Sixteen patients obtained satisfactory results.</p><p><b>CONCLUSION</b>IsoC-3D navigation assisted percutaneous pedicle screw fixation,vertebral canal decompression and interbody fusion using Mast Quadrant system could achieve satisfactory clinical results in treating lumbar degenerative disease and may be a better alternative to conventional surgical procedures. It has advantages such as limited tissue damage, less blood loss, short time in hospital.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Intervertebral Disc Degeneration , General Surgery , Lumbar Vertebrae , General Surgery , Minimally Invasive Surgical Procedures , Methods , Spinal Fusion , Methods , Spinal Stenosis , General Surgery , Surgery, Computer-Assisted , Methods
15.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 27(3): 213-7, 2011 May.
Article in Chinese | MEDLINE | ID: mdl-21838004

ABSTRACT

OBJECTIVE: To observe the effect of different concentration of Tamoxifen ointment on the fibroblasts and transforming growth factor (TGF-beta2) of hypertrophic scar at rabbit ears, so as to explore the possibility of treatment of hypertrophic scar with Tamoxifen. METHODS: The hypertrophic scar model was established in 96 New Zealand rabbits' ears. The wounds were divided into four groups (A, B, C and D), with 144 wounds in each group. Different concentration of tamoxifen ointment (0.5%, 1%, 2%) was topically administered in groups A, B and C respectively, and blank ointment in group D. On postoperative day 30, 60 and 90, the scar samples were harvested. The scar thickness, scar histological change and the content of TGF-beta2 were detected. RESULTS: (1) On the 30th day after operation, the difference of scar tissue thickness among groups A, D and B, C reached statistical significance (group A, D < group B < group C). However, there was a contrary tendency in fibroblasts density and TGF-beta2 content of the scar tissue simultaneously. (2) On 60th, 90th day after injury, there was statistical difference in scar thickness, fibroblasts density and the content of TGF-beta2 in scar of four groups (P < 0.05). The content of TGF-beta2 in group A, B, C, D was (43.97 +/- 3.63) microg/L, (41.92 +/- 3.91) microg/L, (36.69 +/- 4.15) microg/L, (54.90 +/- 4.71) microg/L, respectively, on 60th day; and (45.69 +/- 2.63) microg/L, (40.43 +/- 3.87) microg/L, (38.76 +/- 3.24) microg/L, (52.59 +/- 4.92) microg/L, respectively, on 90th day. The fibroblasts density of scar in groups A, B, C, D was (4392.07 +/- 327.84) point/mm2, (4208.57 +/- 329.76) point/mm2 (4 033.44 +/- 427.91) point/mm2, (4863.03 +/- 387.98) point/mm2, respectively, on 60th day; and (4418.41 +/- 432.52) point/mm2, (4077.65 +/- 386.70) point/mm2, (3844.53 +/- 354.29) point/mm2, (4838.64 +/- 390.52) point/mm2, respectively, on 90th day. The content of TGF-beta2 and fibroblasts density of scar were lined up as group D > group A > group B > group C (P < 0.05). CONCLUSIONS: Topical Tamoxifen can reduce the content of TGF-beta2 and fibroblast, decrease fibroblasts density and the formation of hypertrophic scar at rabbit ears. It offers a new way for the treatment of the hypertrophic scar.


Subject(s)
Cicatrix, Hypertrophic/metabolism , Ear Diseases/metabolism , Tamoxifen/pharmacology , Transforming Growth Factor beta2/metabolism , Animals , Cicatrix, Hypertrophic/drug therapy , Cicatrix, Hypertrophic/pathology , Disease Models, Animal , Ear Diseases/drug therapy , Ear Diseases/pathology , Fibroblasts/drug effects , Fibroblasts/pathology , Ointments , Rabbits
16.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-246952

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of different concentration of Tamoxifen ointment on the fibroblasts and transforming growth factor (TGF-beta2) of hypertrophic scar at rabbit ears, so as to explore the possibility of treatment of hypertrophic scar with Tamoxifen.</p><p><b>METHODS</b>The hypertrophic scar model was established in 96 New Zealand rabbits' ears. The wounds were divided into four groups (A, B, C and D), with 144 wounds in each group. Different concentration of tamoxifen ointment (0.5%, 1%, 2%) was topically administered in groups A, B and C respectively, and blank ointment in group D. On postoperative day 30, 60 and 90, the scar samples were harvested. The scar thickness, scar histological change and the content of TGF-beta2 were detected.</p><p><b>RESULTS</b>(1) On the 30th day after operation, the difference of scar tissue thickness among groups A, D and B, C reached statistical significance (group A, D < group B < group C). However, there was a contrary tendency in fibroblasts density and TGF-beta2 content of the scar tissue simultaneously. (2) On 60th, 90th day after injury, there was statistical difference in scar thickness, fibroblasts density and the content of TGF-beta2 in scar of four groups (P < 0.05). The content of TGF-beta2 in group A, B, C, D was (43.97 +/- 3.63) microg/L, (41.92 +/- 3.91) microg/L, (36.69 +/- 4.15) microg/L, (54.90 +/- 4.71) microg/L, respectively, on 60th day; and (45.69 +/- 2.63) microg/L, (40.43 +/- 3.87) microg/L, (38.76 +/- 3.24) microg/L, (52.59 +/- 4.92) microg/L, respectively, on 90th day. The fibroblasts density of scar in groups A, B, C, D was (4392.07 +/- 327.84) point/mm2, (4208.57 +/- 329.76) point/mm2 (4 033.44 +/- 427.91) point/mm2, (4863.03 +/- 387.98) point/mm2, respectively, on 60th day; and (4418.41 +/- 432.52) point/mm2, (4077.65 +/- 386.70) point/mm2, (3844.53 +/- 354.29) point/mm2, (4838.64 +/- 390.52) point/mm2, respectively, on 90th day. The content of TGF-beta2 and fibroblasts density of scar were lined up as group D > group A > group B > group C (P < 0.05).</p><p><b>CONCLUSIONS</b>Topical Tamoxifen can reduce the content of TGF-beta2 and fibroblast, decrease fibroblasts density and the formation of hypertrophic scar at rabbit ears. It offers a new way for the treatment of the hypertrophic scar.</p>


Subject(s)
Animals , Rabbits , Cicatrix, Hypertrophic , Drug Therapy , Metabolism , Pathology , Disease Models, Animal , Ear Diseases , Drug Therapy , Metabolism , Pathology , Fibroblasts , Pathology , Ointments , Tamoxifen , Pharmacology , Transforming Growth Factor beta2 , Metabolism
17.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-959184

ABSTRACT

@#ObjectiveTo explore the relationship between the motor cognitive ability and the incidence of fall in the aged people. MethodsAccording to the fall experience within a year, 91 aged people were divided into 2 groups: fall group and non-fall group. They were tested using the Timed Up and Go Test (TUGT) with the eyes opening or closing. The anticipated time, actual time and the difference were recorded. ResultsThere were significant differences between these 2 groups in the eye-closed anticipated time, actual time, the difference of eye-open anticipated time and eye-closed anticipated time, the difference of eye-open anticipated time and actual time (P<0.05). ConclusionThe motor cognitive ability of the falled elderly more depends on their vision. The motor cognitive disability is one of the factors resulted in fall in aged people.

18.
Zhonghua Zheng Xing Wai Ke Za Zhi ; 25(4): 277-9, 2009 Jul.
Article in Chinese | MEDLINE | ID: mdl-19873720

ABSTRACT

OBJECTIVE: To explore the ultrastructure of the palate-maxillary sutures under tensile forces by transmission electron microscope (TEM). METHODS: The Suture expanders were made in NiTi-Shape memory alloy (NiTi-SMA). The maximum force was 3.5 N. Fourteen 8-month old mongrel dogs were used in the study. They were divided into three groups, (1) experimental group, (2) control group, (3) sham group. In the experiment and control groups, an 8 mm wide cleft was made by surgery. The suture expanders were fixed onto the palatine bones of the experimental group. The dogs of the experimental group were executed after 3, 7, 14, 28, 56 days of suture expansion. The change of suture tissue was examined by TEM. RESULTS: The cleft of the experiment group were closed at the ninth day of expansion. At the beginning, tissue rupture, exudation, death of fibroblasts, disruption of collagen and tear vessels were seen at the early stage of suture expansion. Then highly active functional manifestations were seen in both osteocytic and fibrocytic populations. At last, normal structure restored. CONCLUSIONS: Cell types and functional condition could be distinguished clearly by TEM. It suggests that the suture expansion should be the process of tissue repair and regeneration. The suture cells response, especially, the osteogenic response were the major factor of increasing suture width.


Subject(s)
Alloys , Maxilla/ultrastructure , Osteogenesis, Distraction , Palate, Hard/ultrastructure , Animals , Bone Regeneration , Cranial Sutures/surgery , Dogs , Maxilla/surgery , Microscopy, Electron, Transmission , Nickel , Osteogenesis , Palate, Hard/surgery , Tensile Strength , Titanium
19.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-328685

ABSTRACT

<p><b>OBJECTIVE</b>To explore the ultrastructure of the palate-maxillary sutures under tensile forces by transmission electron microscope (TEM).</p><p><b>METHODS</b>The Suture expanders were made in NiTi-Shape memory alloy (NiTi-SMA). The maximum force was 3.5 N. Fourteen 8-month old mongrel dogs were used in the study. They were divided into three groups, (1) experimental group, (2) control group, (3) sham group. In the experiment and control groups, an 8 mm wide cleft was made by surgery. The suture expanders were fixed onto the palatine bones of the experimental group. The dogs of the experimental group were executed after 3, 7, 14, 28, 56 days of suture expansion. The change of suture tissue was examined by TEM.</p><p><b>RESULTS</b>The cleft of the experiment group were closed at the ninth day of expansion. At the beginning, tissue rupture, exudation, death of fibroblasts, disruption of collagen and tear vessels were seen at the early stage of suture expansion. Then highly active functional manifestations were seen in both osteocytic and fibrocytic populations. At last, normal structure restored.</p><p><b>CONCLUSIONS</b>Cell types and functional condition could be distinguished clearly by TEM. It suggests that the suture expansion should be the process of tissue repair and regeneration. The suture cells response, especially, the osteogenic response were the major factor of increasing suture width.</p>


Subject(s)
Animals , Dogs , Alloys , Bone Regeneration , Cranial Sutures , General Surgery , Maxilla , General Surgery , Microscopy, Electron, Transmission , Nickel , Osteogenesis , Osteogenesis, Distraction , Palate, Hard , General Surgery , Tensile Strength , Titanium
20.
Zhonghua Shao Shang Za Zhi ; 24(4): 272-4, 2008 Aug.
Article in Chinese | MEDLINE | ID: mdl-19102983

ABSTRACT

OBJECTIVE: To construct of tissue engineering skin including active composite dermal matrix. METHODS: The human fibroblasts and bovine collagen with type I were inoculated on the surface of porcine acellular dermal matrix (PADM) for construction of active dermal substitute, then epidermal cells were inoculated on the dermal matrix for gas-liquid interface culture. The tissue-engineering skin was observed by histological examinations. RESULTS: The structure of fibroblasts in collagen was intact, which was used to construct composite dermal matrix with PADM. The epithelial structure of tissue-engineering skin was similar to that of normal skin with good cell differentiation. Some phenomena were showed in epidermis: basic layer, stratum spinosum, granular layer and stratum corneum, desmosomes. CONCLUSION: Fibroblasts-Collagen-PADM can be an optimal dermal matrix for construction of tissue-engineering skin.


Subject(s)
Dermis/transplantation , Extracellular Matrix/transplantation , Skin, Artificial , Tissue Engineering , Animals , Cattle , Cell Culture Techniques , Collagen Type I , Epidermal Cells , Fibroblasts/cytology , Humans , Skin/cytology , Swine
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