ABSTRACT
The leukemia is a neoplastic clonal disease of hematopoietic system with primary affection of bone marrow. The modern technologies of automated blood analysis permit to implement a quick primary screening of pathological samples of blood suspicious for presence of blast cells. The application of various analysis techniques (optical, cytochemical, immune phenotypical) with the purpose of detecting blast cells demonstrates their different distribution at the graphics.
Subject(s)
Leukemia, Myeloid, Acute/blood , Leukemia, Myeloid, Acute/diagnosis , Bone Marrow/pathology , HumansABSTRACT
The article discusses the parameters of evaluation of thrombocytes activity detected by hematologic analyzers. The techniques of parameters detection are presented using such devices as XE-5000, XE-2100 (Sysmex), LH750, 780, UniCel DxHTM 800 (Beckman Coulter), Advia 120, 2120 (Siemens). The dependence of normal values of these indicators from age and gender are discussed. A single part is dedicated to such characteristics of pre-analytical stage as impact of different anticoagulants used to stabilize blood, time and conditions of storage of samples before beginning of study of analysis results. The clinical significance of every thrombocytes parameter in particular (MPV, PDW, PCT, MPC, MPM, L-PLT, IPF, PCDW, PMDW) is discussed. The actuality of application of these techniques in assessing thrombocytes nowadays is emphasized.
Subject(s)
Blood Platelets/cytology , Platelet Function Tests/instrumentation , Adolescent , Adult , Age Factors , Anticoagulants/administration & dosage , Blood Platelets/drug effects , Female , Humans , Male , Middle Aged , Platelet Activation , Specimen HandlingABSTRACT
The paper reviews literature updates on the examination of platelets by the flow cytofluorometric technique; it analyzes in detail the effect of various anticoagulants used to stabilize blood, the time and conditions of sample storage at the preanalytical stage of studies on the results of an analysis. Different platelet subpopulations detected by the flow cytofluorometric technique are described and their clinical value is shown. The clinical importance of reticular platelet tests is separately considered. This technique is acknowledged to be currently of urgency in estimation of platelets.
Subject(s)
Anticoagulants/therapeutic use , Autoimmune Diseases/diagnosis , Blood Platelets/cytology , Cardiovascular Diseases/diagnosis , Flow Cytometry , Anticoagulants/pharmacology , Autoimmune Diseases/drug therapy , Autoimmune Diseases/pathology , Autoimmune Diseases/physiopathology , Blood Platelets/drug effects , Blood Preservation , Cardiovascular Diseases/drug therapy , Cardiovascular Diseases/pathology , Cardiovascular Diseases/physiopathology , Cell Separation , Humans , Platelet Activation/drug effects , Platelet CountABSTRACT
The paper reviews literature updates on the organization and specific features of megakaryocytopoiesis, the mechanisms of its regulation, possible ways for platelets to occur from megakaryocytes. It characterizes thrombocytopoiesis, by describing the detailed structural organization of platelets and gives data on the relationship of the morphofunctional features of platelets to the ploidy of megakaryocytes that give rise to the latter. The structure and clinical value of a number of platelet activation markers, such as CD41/CD61, CD42, CD62p, platelet-leukocyte aggregates, microvesicles, are described.
