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1.
Emerg Infect Dis ; 23(6): 931-938, 2017 06.
Article in English | MEDLINE | ID: mdl-28518030

ABSTRACT

Vaccinia virus (VACV) is a zoonotic agent that causes a disease called bovine vaccinia, which is detected mainly in milking cattle and humans in close contact with these animals. Even though many aspects of VACV infection have been described, much is still unknown about its circulation in the environment and its natural hosts/reservoirs. To investigate the presence of Orthopoxvirus antibodies or VACV DNA, we captured small rodents and marsupials in 3 areas of Minas Gerais state, Brazil, and tested their samples in a laboratory. A total of 336 animals were tested; positivity ranged from 18.1% to 25.5% in the 3 studied regions located in different biomes, including the Atlantic Forest and the Cerrado. Analysis of nucleotide sequences indicated co-circulation of VACV groups I and II. Our findings reinforce the possible role played by rodents and marsupials in VACV maintenance and its transmission chain.


Subject(s)
Antibodies, Viral/blood , Cattle Diseases/epidemiology , DNA, Viral/blood , Disease Outbreaks , Marsupialia/virology , Rodentia/virology , Vaccinia/epidemiology , Animals , Brazil/epidemiology , Cattle , Cattle Diseases/blood , Cattle Diseases/transmission , Disease Reservoirs/virology , Incidence , Molecular Typing , Vaccinia/blood , Vaccinia/transmission , Vaccinia/veterinary , Vaccinia virus/classification , Vaccinia virus/genetics , Vaccinia virus/pathogenicity
3.
PLoS Negl Trop Dis ; 9(6): e0003870, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26083967

ABSTRACT

BACKGROUND: DNA repair mechanisms are crucial for maintenance of the genome in all organisms, including parasites where successful infection is dependent both on genomic stability and sequence variation. MSH2 is an early acting, central component of the Mismatch Repair (MMR) pathway, which is responsible for the recognition and correction of base mismatches that occur during DNA replication and recombination. In addition, recent evidence suggests that MSH2 might also play an important, but poorly understood, role in responding to oxidative damage in both African and American trypanosomes. METHODOLOGY/PRINCIPAL FINDINGS: To investigate the involvement of MMR in the oxidative stress response, null mutants of MSH2 were generated in Trypanosoma brucei procyclic forms and in Trypanosoma cruzi epimastigote forms. Unexpectedly, the MSH2 null mutants showed increased resistance to H2O2 exposure when compared with wild type cells, a phenotype distinct from the previously observed increased sensitivity of T. brucei bloodstream forms MSH2 mutants. Complementation studies indicated that the increased oxidative resistance of procyclic T. brucei was due to adaptation to MSH2 loss. In both parasites, loss of MSH2 was shown to result in increased tolerance to alkylation by MNNG and increased accumulation of 8-oxo-guanine in the nuclear and mitochondrial genomes, indicating impaired MMR. In T. cruzi, loss of MSH2 also increases the parasite capacity to survive within host macrophages. CONCLUSIONS/SIGNIFICANCE: Taken together, these results indicate MSH2 displays conserved, dual roles in MMR and in the response to oxidative stress. Loss of the latter function results in life cycle dependent differences in phenotypic outcomes in T. brucei MSH2 mutants, most likely because of the greater burden of oxidative stress in the insect stage of the parasite.


Subject(s)
Gene Expression Regulation/physiology , MutS Homolog 2 Protein/metabolism , Trypanosoma brucei brucei/metabolism , Trypanosoma cruzi/metabolism , Animals , DNA, Protozoan/genetics , Gene Deletion , Guanine/analogs & derivatives , Guanine/metabolism , Microsatellite Repeats , MutS Homolog 2 Protein/genetics , Oxidative Stress , Protein Transport , RNA Interference , Trypanosoma brucei brucei/genetics , Trypanosoma cruzi/genetics
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