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1.
Chem Commun (Camb) ; 53(70): 9721-9724, 2017 Aug 29.
Article in English | MEDLINE | ID: mdl-28782763

ABSTRACT

An on-surface isothermal helicase-dependent amplification is devised for simple, point-of-need quantification of bacterial genomes. The method relies on the enzyme-extension of a thiol-modified reverse primer anchored to indium tin oxide electrodes, which shows strikingly high thermal and storage stability. Amplification and electrochemical detection of only 10 genomes are thus performed on the same platform without thermal cycling.


Subject(s)
DNA Helicases/metabolism , Electrochemical Techniques , Enzymes, Immobilized/metabolism , Nucleic Acid Amplification Techniques , Oligonucleotides/chemistry , Salmonella/isolation & purification , Tin Compounds/chemistry , Electrodes , Salmonella/genetics
2.
Biosens Bioelectron ; 26(5): 2396-401, 2011 Jan 15.
Article in English | MEDLINE | ID: mdl-21067909

ABSTRACT

Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA(21)) adsorbed on carbon paste electrodes for the assessment of the antioxidant capacity is proposed. The method was based on the partial damage of a DNA layer adsorbed on the electrode surface by OH radicals generated by Fenton reaction and the subsequent electrochemical oxidation of the intact adenine bases to generate an oxidation product that was able to catalyze the oxidation of NADH. The presence of antioxidant compounds scavenged hydroxyl radicals leaving more adenines unoxidized, and thus, increasing the electrocatalytic current of NADH measured by differential pulse voltammetry (DPV). Using ascorbic acid (AA) as a model antioxidant species, the detection of as low as 50 nM of AA in aqueous solution was possible. The protection efficiency was evaluated for several antioxidant compounds. The biosensor was applied to the determination of the total antioxidant capacity (TAC) in beverages.


Subject(s)
Antioxidants/analysis , Antioxidants/chemistry , Beverages/analysis , Biosensing Techniques/instrumentation , Conductometry/instrumentation , DNA/chemistry , Food Analysis/instrumentation , Catalysis , DNA Damage , Equipment Design , Equipment Failure Analysis
3.
Anal Bioanal Chem ; 378(8): 1922-8, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15064901

ABSTRACT

This article summarises our work on the development of voltammetric sensors based on molecularly imprinted polymers. Several recognition elements and integration strategies were used:1.membranes electropolymerised at the electrode surface; 2.casting of polymeric membranes by drop-coating a solution of pre-formed polymer (polyphosphazene) and template in a low-boiling-point solvent on to the electrode surface; 3.preparation of composite membranes containing conductive material (graphite or carbon black), acrylic-type molecularly imprinted polymers (small particle size), and PVC as binder; and 4.in-situ polymerisation of a thin layer of acrylic imprinted polymer deposited on the electrode surface by spin coating. All the options evaluated offer the possibility of controlling electrode characteristics such as hydrophobic/hydrophilic character, permeability, or film thickness, which are essential for obtaining good sensor performance.


Subject(s)
Chemistry Techniques, Analytical/instrumentation , Chemistry Techniques, Analytical/methods , Polymers/chemistry , Polymers/chemical synthesis , Electrochemistry , Electrodes , Molecular Structure , Organophosphorus Compounds/chemistry , Plastics/chemical synthesis , Plastics/chemistry
4.
Biosens Bioelectron ; 18(4): 353-62, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12604252

ABSTRACT

Despite the increasing number of applications of molecularly imprinted polymers (MIPs) in analytical chemistry, the construction of a biomimetic voltammetric sensor remains still challenging. This work investigates the development of a voltammetric sensor for vanillylmandelic acid (VMA) based on acrylic MIP-modified electrodes. Thin layers of MIPs for VMA have been prepared by spin coating the surface of a glassy carbon electrode with the monomers mixture (template, methacrylic acid, a cross-linking agent and solvent), followed by in situ photopolymerisation. After extraction of the template molecule, the peak current recorded with the imprinted sensor after rebinding was linear with VMA concentration in the range 19-350 microg ml(-1), whereas the response of the control electrode is independent of incubation concentration, and was about one-tenth of the value recorded with the imprinted sensor at the maximum concentration tested. Under the conditions used, the sensor is able to differentiate between VMA and other closely structural-related compounds, such as 3-methoxy-4-hydroxyphenylethylene glycol (not detected), or 3,4- and 2,5-dihydroxyphenilacetic acids, which are adsorbed on the bare electrode surface but not at the polymer layer. Homovanillic acid was detected with the imprinted sensors after incubation, indicating that the presence of both methoxy and carboxylic groups in the same position as in VMA is necessary for effective binding in the imprinted sites. Nevertheless, both species can be differentiated by the oxidation potential. It can be concluded that MIP-based voltammetric electrodes are very promising analytical tool for the development of highly selective analytical sensors.


Subject(s)
Biomimetic Materials/chemical synthesis , Coated Materials, Biocompatible/chemical synthesis , Electrochemistry/instrumentation , Electrodes , Methacrylates , Transducers , Vanilmandelic Acid/analysis , Electrochemistry/methods , Equipment Design , Equipment Failure Analysis , Polymers , Reproducibility of Results , Sensitivity and Specificity
5.
Anal Chem ; 72(3): 520-7, 2000 Feb 01.
Article in English | MEDLINE | ID: mdl-10695137

ABSTRACT

The electrochemical oxidation of the adenine moiety in NAD+ and other adenine nucleotides at carbon paste electrodes gives rise to redox-active products which strongly adsorb on the electrode surface. Carbon paste electrodes modified with the oxidation products of NAD+ show excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 400 mV. The rate constant for the catalytic oxidation of NADH, determined by rotating disk electrode measurements and extrapolation to zero concentration of NADH, was found to be 2.5 x 10(5) M-1 s-1. The catalytic oxidation current allows the amperometric detection of NADH at an applied potential of +50 mV (Ag/AgCl) with a detection limit of 4.0 x 10(-7) M and linear response up to 1.0 x 10(-5) M NADH. These modified electrodes can be used as amperometric transducers in the design of biosensors based on coupled dehydrogenase enzymes and, in fact, we have designed an amperometric biosensor for glycerol based on the glycerol dehydrogenase (GlDH) system. The enzyme GlDH and its cofactor NAD+ were co-immobilized in a carbon paste electrode using an electropolymerized layer of nonconducting poly(o-phenylenediamine) (PPD). After partial oxidation of the immobilized NAD+, the modified electrode allows the amperometric detection of the NADH enzymatically obtained at applied potential above 0 V (Ag/AgCl). The resulting biosensor shows a fast and linear response to glycerol within the concentration range of 1.0 x 10(-6)-1.0 x 10(-4) M with a detection limit of 4.3 x 10(-7) M. The amperometric response remains stable for at least 3 days. The biosensor was applied to the determination of glycerol in a plant-extract syrup, with results in good agreement with those for the standard spectrophotometric method.


Subject(s)
Biosensing Techniques/methods , Glycerol/analysis , NAD/analysis , Carbon/chemistry , Catalysis , Electrodes , Feasibility Studies , NAD/chemistry , Oxidation-Reduction , Oxidoreductases/chemistry , Plant Extracts/chemistry
6.
Biosens Bioelectron ; 12(8): 739-47, 1997.
Article in English | MEDLINE | ID: mdl-9421885

ABSTRACT

A glutamate biosensor was developed by electropolymerizing o-phenylenediamine on a dehydrogenase-NAD+ modified carbon paste electrode. The amperometric response to glutamate was obtained by means of the electrocatalytic oxidation of the enzymatically produced NADH, at an applied potential close to 0 V (Ag/AgCl). After optimizing carbon paste composition, polymer thickness and operating variables, a linear amperometric response to glutamate was obtained within the concentration range 5.0 x 10(-6)-7.8 x 10(-5) M with a detection limit of 3.8 x 10(-6) M. The biosensor was applied to the determination of glutamate in chicken bouillon cubes. Good accuracy was found with respect to a reference enzymatic spectrophotometric method.


Subject(s)
Biosensing Techniques , Glutamic Acid/analysis , Phenylenediamines , Carbon , Electrodes
7.
Talanta ; 43(5): 779-84, 1996 May.
Article in English | MEDLINE | ID: mdl-18966548

ABSTRACT

A yeast alcohol dehydrogenase amperometric carbon paste-based biosensor, with Meldola Blue as a mediator and a dialysis membrane with a very small molecular weight cut-off for protection, is described. The influence of membrane pore size on the stability and overall kinetics of the biosensor is shown using cyclic voltammetry and stationary potential measurements. The operating potential is + 50 mV vs. Ag/AgCl, KCl sat. reference electrode. Application of this device to the determination of ethanol in alcoholic beverages was achieved successfully. In these kinds of samples and at this working potential no interferences were found.

8.
Talanta ; 43(5): 785-96, 1996 May.
Article in English | MEDLINE | ID: mdl-18966549

ABSTRACT

A reagentless uric acid selective biosensor constructed by immobilising uricase and horseradish peroxidase (HRP) in carbon paste without the addition of an electron transfer mediator is described. The response of the electrode is based on the enzymatic reduction of hydrogen peroxide in the presence of uric acid. Uricase and HRP were dispersed in the carbon paste and the optimum paste mixture was determined. Poly(o-aminophenol) was electropolymerised at the working surface area of the electrode acting as a conducting polymer layer. Cyclic voltammetry was used to characterise the permselective characteristics of the polymer layer. At an applied potential of 50 mV vs. Ag/AgCl, a linear response was obtained up to 1 x 10(-4) M, with a limit of detection of 3 x 10(-6) M. The sensor had a response time of 37 s. a calibration precision of 2.2% (n = 4) and an estimated sample frequency of 20 h(-1). Responses to the analyte of interest were pH dependent. The sensor was incorporated into a flow injection system for the qualification of uric acid in human serum. Results compared favourably with a standard spectrophotometric method.

9.
J Pharm Biomed Anal ; 14(1-2): 57-63, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8833967

ABSTRACT

A detailed study of the electrochemistry of clenbuterol at bare carbon-paste electrodes (CPEs) has been carried out. Results showed that clenbuterol undergoes an ECE process. This compound is irreversibly oxidised at high potentials, resulting in the formation of a product which demonstrates quasi-reversible electrochemical behaviour at less positive potentials. The amount of this chemical product formed is very pH-dependent. Investigations into the electrochemical behaviour of clenbuterol at Nafion-modified CPEs were also made. The use of a thin Nafion film cast over the CPE resulted in a large increase in peak current over bare electrodes. Linear accumulation occurred with time, the linear range increasing with decreasing concentration. This allowed the detection of low concentrations of clenbuterol. Diffusion proved to be the rate-controlling process of clenbuterol through the Nafion membrane.


Subject(s)
Clenbuterol/chemistry , Carbon , Electrochemistry , Electrodes , Fluorocarbon Polymers , Hydrogen-Ion Concentration , Indicators and Reagents/analysis , Oxidation-Reduction
10.
Analyst ; 115(9): 1215-7, 1990 Sep.
Article in English | MEDLINE | ID: mdl-2091490

ABSTRACT

The electrochemical behaviour of pipemidic acid (8-ethyl-5,8-dihydro-5-oxo-2-(1-piperazinyl)-pyrido[2,3-d]pyrimidine-6- carboxylic acid), a well known antimicrobial agent used for urinary infections, was investigated by linear-sweep, differential-pulse and square-wave voltammetry at a hanging mercury drop electrode. Two reduction processes were observed in Britton-Robinson buffers at acid pH, whereas only one or two processes were observed in alkaline solutions, dependent on the pH of the buffer employed. Adsorptive effects were used to accumulate the drug on to the electrode. The adsorbed species were measured voltammetrically by using a cathodic process appearing at -0.76 V in 0.1 M HCIO4. Linear calibration graphs were obtained in the range 2.5 x 10(-9)-2.0 x 10(-7) M. A simple procedure of extraction was employed for the determination of the drug in urine samples.


Subject(s)
Pipemidic Acid/urine , Adsorption , Electrochemistry , Humans , Hydrogen-Ion Concentration
11.
J Pharm Biomed Anal ; 6(6-8): 743-7, 1988.
Article in English | MEDLINE | ID: mdl-16867338

ABSTRACT

Folic acid was voltammetrically measured after preconcentration by adsorption at the static mercury drop electrode. Phase selective AC voltammetry provided the most sensitive stripping signal. After previous analyte extraction, using liquid-solid extraction with a C-18 reversed phase cartridge, the method was suitable for folic acid measurement in human serum. The detection limit was 5.9 x 10(-9) M with an overall precision of 9.9% (R.S.D.; n = 7) at the concentration level of 1.0 x 10(-7) M with a mean recovery of 57%.

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