ABSTRACT
Zinc oxide nanoparticles (ZnO-NPs) are multifunctional oxide metal nanoparticles that have been considered by many due to their unique properties including UV filtration, semiconducting, antimicrobial, antifungal, and photocatalytic activity. In the present study, ZnO-NPs have been synthesized from Zn(SO4)·7H2O through the use of "green synthesis" method that utilizes Prosopis farcta aqueous extract. The characterization of synthesized ZnO-NPs have been performed by UV-Vis absorption, Fourier-Transform Infrared spectroscopy (FT-IR), Raman, Powder X-ray Diffraction (PXRD), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray analysis (EDX). Results have illustrated that the synthesized nanoparticles contain a hexagonal shape and sheet form with sizes of 40-80â¯nm. The antifungal activity of ZnO-NPs against Candida albicans has shown that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were 128 and 256⯵g/ml, respectively. The cytotoxicity activity of synthesized nanoparticles was tested on breast cancer cells (MCF7), while the IC50 was calculated to be 90⯵g/ml.
Subject(s)
Antifungal Agents/chemistry , Antifungal Agents/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Metal Nanoparticles/chemistry , Zinc Oxide/chemistry , Breast Neoplasms/drug therapy , Candida albicans/drug effects , Female , Green Chemistry Technology/methods , Humans , MCF-7 Cells , Microbial Sensitivity Tests/methods , Microscopy, Electron, Scanning/methods , Microscopy, Electron, Transmission/methods , Particle Size , Plant Extracts/chemistry , Plant Extracts/pharmacology , Spectrometry, X-Ray Emission/methods , X-Ray Diffraction/methodsABSTRACT
The evaluation of cytotoxic and apoptotic activities of silver nanoparticles (Ag-NPs) synthesized by aqueous extract of Prosopis farcta was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic activity was tested using TUNEL method. The obtained results of MTT showed that the cell viability of A549 was dependent on the nanoparticles concentration and incubation time. Therefore, although the cytotoxic effect increased as the Ag-NPs concentration and incubation time heightened, yet the viability of HT-29 cells seems to be dependent only on the incubation time. The apoptotic results of the nanoparticles showed more than 50% of apoptosis on A549 and HT-29 cell lines, which in this case, HT-29 demonstrated 100% apoptosis at concentrations of more than 400 µg/ml. It seems that Ag-NPs synthesized using P. farcta extract can serve as anti-cancer agent in the treatment many cancers through creating or discovering new drug forms.
