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1.
Arch Esp Urol ; 47(4): 343-7, 1994 May.
Article in Spanish | MEDLINE | ID: mdl-8053720

ABSTRACT

There is currently no existing methodology for evaluating normal exocrine function in the healthy rat. Consequently, most of the experimental studies that have been conducted are incomplete. For this reason, we developed an experimental model to investigate the testicular exocrine function using spermatozoa obtained from the tail of the epididymis of male rats of different ages. The methodology is described and the results obtained relative to sperm count, survival time, percent mobile/immobile spermatozoa and presence of abnormal forms are presented and compared with the data from experimental studies using electroejaculation techniques. Similarly, the advantages and disadvantages of the method are discussed. In our view, the methodology described herein can also be utilized in other laboratory animals.


Subject(s)
Rats, Wistar/physiology , Spermatozoa/abnormalities , Spermatozoa/physiology , Testis/physiology , Animals , Male , Rats
2.
Actas Urol Esp ; 18(3): 249-52, 1994 Mar.
Article in Spanish | MEDLINE | ID: mdl-8036958

ABSTRACT

Men with a background of unilateral torsion of testis (t.t.) show a greater percentage of infertility and changes in the spermiogram. Currently, the cause of contralateral testicle affectation remains unknown. With the aim of verify whether these changes are secondary to an immunological mechanism, the authors conducted experimental work in 3 groups of male rats, inducing a right t.t. over 24 hours, followed by detorsion and preservation of contorted testicle. Immunological response is determined through direct and indirect immunofluorescence reactions, including a brief description of the technique used. Positive reactions were obtained in both the spermatozoa's head and flagellum, more frequently in animals killed at 30 days and gradually decreasing in those killed at 60 and 90 days. The authors compare their results to other experimental works, and recommend to perform orchiectomy because of the damaging effect of the contorted testicle over the contralateral one.


Subject(s)
Spermatic Cord Torsion/immunology , Animals , Humans , Male , Rats , Rats, Wistar , Spermatozoa , Time Factors
3.
Cir Pediatr ; 5(1): 32-6, 1992 Jan.
Article in Spanish | MEDLINE | ID: mdl-1567746

ABSTRACT

The unilateral testicular torsion reflects in a negative manner on the contralateral testis. With the objective of proving if these alterations can be avoided, we used two groups of rats. The problem group was subjected to torsion with posterior orchiectomy. The control of spermatozoon we found significant differences for the spermatozoon with bigger motility. These results are from different from those obtained in animals submitted to testicular torsion with posterior distortion leaving the testis twisted. We think the orchiectomy has a prophylactic value upon the perturbation in the contralateral testis.


Subject(s)
Orchiectomy , Spermatic Cord Torsion/surgery , Testis/physiology , Animals , Male , Rats , Rats, Inbred Strains , Sperm Count , Sperm Motility
4.
Cir Pediatr ; 2(1): 29-33, 1989 Jan.
Article in Spanish | MEDLINE | ID: mdl-2485660

ABSTRACT

The unilateral testicular torsion produce alteration of contralateral testes, with a frequence of sterility and alteration sperm bigger than normal population. The great differences report for various authors in the works make necessary to know better the pathogenesis of the testicular torsion about the fertility. With the aim to study this aspect, we carry out this experimental work in that animals with testicular torsion, although with individual results very different, have, again the animals with sham intervention, less number of sperm (P less than 0.001), with less time of survivorship (P less than 0.001), bigger percentage of precocious agglutination and abnormal shape (P less than 0.001) and bigger quantity of immobile sperm. These alteration as adult animals, but they are bigger in the late.


Subject(s)
Spermatic Cord Torsion/physiopathology , Testis/physiopathology , Animals , Male , Rats , Rats, Inbred Strains , Sperm Agglutination , Sperm Count , Sperm Motility , Spermatic Cord Torsion/pathology , Spermatozoa/abnormalities
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