[The sequence coverage in different methods of mass spectrometry data analysis obtained on model proteins]. / Stepen' pokrytiia aminokislotnoi posledovatel'nosti pri ispol'zovanii razlichnykh metodov analiza mass-spektrometricheskikh dannykh, poluchennykh na model'nykh belkakh.

The aim of this study was to evaluate sequence coverage of five model proteins (CYB5A, SMAD4, RAB27B, FECH, and CXXC1) by means of shotgun proteomic data analysis employing different methods of data treatment including database-dependent search engines (MASCOT and X!Tandem) and de novo sequencing software ((PEAKS, Novor, and PepNovo+). In order to achieve maximal results, multiprotease hydrolysis including enzymes trypsin, LYS-C, ASPN and GluC was performed in solution and using the FASP method. High resolution mass spectrometry was carried out with a Q EXACTIVE HF hybrid mass spectrometer in the positive ionization mode; parent ions with the highest intensity and a charge range from +2 to +6 were fragmented in the HCD mode. 27 experiments were carried out (hydrolysis with each of 5 enzymes in solution, 4 for the FASP protocol, three technical repeats). Using parameters limiting false identification of peptides, the search engines and de novo sequencing software gave similar results. The degree of sequence coverage was not at least 40%, and in the best cases it reached 80-90%. The use of de novo sequencing software resulted in identification of the Y12H amino acid substitution in one model protein (CYB5A).

[The substantiation of organizational and methodological approaches to the development of standards for the supply of medical equipment to provide educational activities in the Military Medical Academy].

The article shows that one of the conditions for qualitative training of specialists for the medical service of the Armed Forces of the Russian Federation is the availability of a well-equipped material and technical (teaching and material) base, the main element of which is medical property. It was revealed that at present the nomenclature, quantity and medical and technical characteristics of medical educational material for implementation of educational activities in the Military Medical Academy are not defined by normative legal acts. It is established that the formation of the material and technical (educational- material) base academy should be carried out on the basis of the principle of standardized allowance, which will allow the military management bodies to plan the allocation of not only the relevant medical educational equipment, but also the necessary amounts of money.

Modern aproaches to medical equipment for supplies teams of specialized medical care.

The article describes the methodological approaches to medical equipment for supplies teams of specialized medical care and medical and nursing teams, formed on the basis of the Military Medical Academy. Kirov. It is shown that the priority in the selection of samples medical equipment suitable for use in the medical unit formations, military units and military medical institutions and medical institutions (subdivisions) of other ministries and departments, belongs to medical bags, sets of medical equipment, sets and packing medical taken to supply the Armed Forces and included in the rations medical supplies formations, military units and organizations. A brief description of sample medical equipment used to equip teams.

[Software and hardware system for medical equipment recording in a military field hospital].

Software and hardware system for medical equipment recording in a military field hospital. The article provides characteristics on the modern software and hardware system for recording of medical equipment in a military field during military time. This system is included into the assembly of medical equipment , included in the norms of medical equipment supply for military field hospitals. Authors give an algorithm of development and use of specialized software for recording of-medical equipment in a military field hospital.

[Improvement of medical equipment setting for the hospital link of the medical service during wartime].

One of the priorities of the military health care is to improve the system of rationing medical equipment for the hospital unit of the medical service of the Armed Forces in wartime. This is determined the fact that the effectiveness of measures to provide military field hospitals with medical supplies depends on the quality of medical care for the wounded and sick, as well as the level of their return to duty. The article presents the characteristics of modern standards medical supplies procurement of military field hospitals included in the new regulatory legal act of the Russian Federation Ministry of Defence--"Standards of supplies medical supplies medical and pharmaceutical organizations (units) of the Russian Federation on the wartime armed forces", approved and put into effect in 2015 by order of the Minister of Defence of the Russian Federation.

[Possibilities of pharmacotherapy of diabetes acute complications when delivering urgent and emergency aid].

The authors give a description of acute complications when delivering urgent and emergency pharmacotherapy. The authors examined normative legal basis, regulating urgent and emergency aid delivery at the army level of the medical service of the Armed Forces of the Russian Federation, and determine the list of medications. Comparative description of pharmacotherapy of acute complications of diabetes is given and determined its possibilities.

[Modern order of rationing of medical property for military medical and pharmaceutical organizations in peacetime].

Modern order of rationing of medical property for military medical and pharmaceutical organizations in peacetime. The characteristic of the new rules of medical supply for medical and pharmaceutical institutions of the Armed Forces in peacetime are presented, approved and put into effect by the order of the Minister of Defence of the Russian Federation. New standards of medical supply logistics adapted to the current organizational structure of the military healthcare and pharmaceutical organizations. Their use in practice will improve the quality of primary specialized healthcare, as well as specialized and high-tech medical care to military personnel and other categories of citizens who are entitled to health care through the Ministry of Defence.

Contribution of the 320th central warehouse to sanitary medical support of the Red'Army during the Great Patriotic war.

During the Great Patriotic war (1941-1945) medical supply experts of the 320th Central warehouse sanitary solved complex problems for the regular and complete provision with medical property guide action by the army, the formation of complete basic equipment, service of medical transport and medical equipment. The experience gained in providing acting army allowed in the post war period progressively develop functioning of the system of medical supplies of troops (forces), successfully and smoothly carried out in the framework of its organizational planning and other activities.

[Comparative characteristics of domestic and foreign means of the first aid on the battlefield and in the epicenter of emergency situation].

Comparative characteristics of domestic and foreign means of the first aid on the battlefield and in the epicentre of emergency situation. The results of comparative analysis of domestic and foreign means of the first aid on the battlefield and in the epicentre of emergency situation are presented. It was found that the first aid means used in the Armed Forces are effective and allow performing specified activities, regardless of the qualifications and assisting skills. Modern innovative means of providing emergency and urgent care may be used on the stages of medical evacuation of military and military hospitals.

Problems and prosnects of innovation development of technical support for military medicine.

Formation of the scientific potential and development of advanced medical equipment samples is one of the priorities for the development ofRussian military medicine. However, the main problem of innovation implementation into the daily routine of medical units formations and military units, as well as military and medical organizations is that the above mentioned innovation is not fully studied. The article defines the main ways of innovation development of technical equipment of military medicine, shows the characteristics of the most advanced models of medical equipment, as well as recommendations for their use to improve the efficiency of medical support of troops (forces) in modern conditions.

Identification of Central Nervous System Proteins in Human Blood Serum and Plasma.

Mass-spectrometric identification of proteins in human blood plasma and serum was performed by comparing mass-spectra of fragmented peptides using Swiss-Prot and UniProtKB databases of amino acid sequences. After choosing the appropriate identification conditions we found that combination of spectrum search parameters are optimal for identification of CNS proteins. In the studied plasma and serum samples, 9 proteins involved into pathological processes in the nervous tissue were identified; 7 of them were identified in both plasma and serum.

[The contributin of medical supply experts into military health care during the Great Patriotic War].

During the Great Patriotic War edical supply experts to solve complex problems for the "smooth and full achievement of medical supplies of the army, the development of official documents, creation of new samples of complete- standard-issue equipment, ambulance and medical equipment. The experience gained on the battlefield and during the post-war period, allowed progressively improving the functioning of. the medical supply troops (forces), successfully and consistently carried out in the framework of its organizational planning and other activities.

[Development and use of modern hardware-software complex for medical equipment accounting in military unit of the medical service in wartime].

Development and use of modem hardware-software complex for medical equipment accounting in military unit of the medical service in wartime. The authors presented characteristics of a modern hardware-software complex for medical equipment assessment in medical troops of the medical service, included into the complete set of medical equipment <, which is accepted by the Armed Forces of the Russian Federation. An algorithm of development and routine of special- purpose software for accounting of medical equipment in medical branches of formations and military units in wartime.

[Implementation of modern approaches to medical equipment of medical service's troops].

Implementation of modern approaches to medical equipment of medical service's troops. The authors give essential characteristics of modern approaches to equipment of medical small and large units and troops. It is shown that for medical evacuation under field conditions it is neccessary to use modern complete-basic equipment that allows effectively providing medical assistance to the wounded and sick, maintaining the established level of readiness of medical troops, unifying norms of medical equipping, operatively deploying organisational stages of medical evacuation, optimizing planning of medical equipment. Medical equipment in peacetime should be carried out on the basis of standards and orders of medical care delivery that ensures harmonization with requirements of public health.

[Medical devices and its in-field use].

The quality of in-field treatment and prevention measures depends on the availability of modern medical devices, instruments, and equipment. In this regard peculiarities of medical care delivery during military conflicts advance special requirements for medical equipment, which is used by the medical service of the Armed Forces of the Russian Federation. The article shows prospects of in-field use of the most important samples of medical devices adopted by the Armed Forces.

[Development of different approaches to classification of medical equipment sets].

The authors provided results of the study on approaches to the classification of medical equipment sets for the medical service of the Armed Forces. A retrospective analysis of the formation and development of classifications of first aid kits, medical bags, sets of medical equipment, medical kits and kits is performed. Basic factors influencing the structure and principles of classification sets of medical equipment are detected. The authors present modern classification of sets of medical equipment and the ways of its improvement.