ABSTRACT
The role of asymptomatic infections caused by Chlamydia trachomatis in male infertility and the efficacy of antibiotics in the treatment of this condition are not yet definitely determined. A total of 165 infertile males having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included. Semen samples were taken from all participants and after analysing for semen parameters, undergone real-time PCR, and reactive oxygen species (ROS) as well as total antioxidant capacity (TAC) assays. Infected individuals of study group were treated with antibiotic. One month after the treatment completion, second semen samples were taken and undergone all the tests mentioned. The frequency of C. trachomatis was significantly higher in the infertile men compared with the fertile ones (4.2% vs 0.6%). Most of the semen parameters were improved and reached their normal range, the level of TAC elevated and ROS level as well as ROS/TAC ratio reduced after antibiotic treatment. Moreover, wives of three infected infertile men (42.9%) became pregnant 4 months after the treatment completion. Our data suggest that asymptomatic infection caused by C. trachomatis is correlated with male infertility and antibiotic therapy can improve the semen quality and fairly treat the male infertility.
ABSTRACT
The objective of this study was to determine the association between vaginal Chlamydia infection and cervical intraepithelial neoplasia (CIN). Data were collected in a case-control study for 60 patients with CIN in biopsy and 85 control subjects with normal colposcopy and biopsy. Serum antibodies to C trachomatis were associated with an increased risk for CIN [odds ratio (OR) = 7.3; 95% confidence interval (CI) 1.5-35.2)]. There was also a significant association between presence of inclusion bodies for C. trachomatis and CIN (OR = 5.5; 95% CI 2.4-12.4). These results indicate a strong association between CIN and chlamydial cervicitis.
Subject(s)
Chlamydia Infections/complications , Chlamydia trachomatis , Uterine Cervical Dysplasia/microbiology , Uterine Cervical Neoplasms/microbiology , Vaginal Diseases/complications , Adult , Aged , Biopsy , Case-Control Studies , Chi-Square Distribution , Chlamydia Infections/diagnosis , Chlamydia Infections/epidemiology , Colposcopy , Female , Humans , Iran/epidemiology , Middle Aged , Risk Factors , Surveys and Questionnaires , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/epidemiology , Vaginal Diseases/diagnosis , Vaginal Diseases/epidemiology , Uterine Cervical Dysplasia/diagnosis , Uterine Cervical Dysplasia/epidemiologyABSTRACT
The objective of this study was to determine the association between vaginal Chlamydia infection and cervical intraepithelial neoplasia [CIN]. Data were collected in a case-control study for 60 patients with CIN in biopsy and 85 control subjects with normal colposcopy and biopsy. Serum antibodies to C. trachomatis were associated with an increased risk for CIN [odds ratio [OR] = 7.3; 95% confidence interval [CI] 1.5-35.2]]. There was also a significant association between presence of inclusion bodies for C. trachomatis and CIN [OR = 5.5; 95% CI 2.4-12.4]. These results indicate a strong association between CIN and chlamydial cervicitis
Subject(s)
Chlamydia trachomatis , Biopsy , Case-Control Studies , Retrospective Studies , Uterine Cervical DysplasiaABSTRACT
Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibiotic susceptibilities and, for comparison, a collection of 80 strains isolated from 1989 to 1991. Erythromycin resistance was uncommon (0.6%), whereas an overall high rate of tetracycline resistance was found, increasing between 1989-1991 and 1995-1997 from 23 to 42%. The tetracycline-resistant strains belonged to more than 10 different T types, the majority being types 4, 11, and B3264. By conventional M typing of 406 tetracycline-resistant isolates, more than 20 different M types were found. Approximately 50% of the strains were nontypeable by T agglutination as well as serological M typing; however, by genotyping by a combined PCR-capture-enzyme-linked immunosorbent assay, many of these strains were successfully emm typed. We conclude that the high rate of tetracycline resistance among Iranian S. pyogenes isolates is due to multiclonal dissemination of resistance within the streptococcal population rather than epidemic spread of single clones.
Subject(s)
Antigens, Bacterial , Bacterial Outer Membrane Proteins , Streptococcal Infections/epidemiology , Streptococcus pyogenes/drug effects , Tetracycline Resistance , Tetracycline/pharmacology , Bacterial Proteins/isolation & purification , Bacterial Typing Techniques , Carrier Proteins/isolation & purification , Child , Electrophoresis, Gel, Pulsed-Field , Erythromycin/pharmacology , Humans , Iran , Streptococcus pyogenes/classification , Streptococcus pyogenes/geneticsABSTRACT
We examined three populations from the Tehran region and the North part of Iran (Gilan), in all more than 5000 individuals, for carriage of Streptococcus pyogenes (group A streptococci; GAS). Children or adults with acute pharyngotonsillitis and healthy school children harboured GAS in 34-1, 20.0 and 21.0%, respectively. Typing of 421 randomly selected isolates showed a predominance of M-types M4, M5, M11, M12, as well as the provisional type 4245; however, many of the isolates were T and M non-typable. Forty-three percent of all strains were opacity factor (OF) negative. The type distribution differed markedly from that reported in 1973-4, when M types 1 and 12 were predominant.
