Polymorphism in neutrophil cytosolic factor 4 (NCF4) of dairy cows had mastitis in previous lactations, and the relationship with the respiratory burst.

Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase), as a key factor in innate immunity, consists of several components, one of them is p40phox which is encoded by neutrophil cytosolic factor 4 (NCF4). Respiratory burst and reactive oxygen species (ROS) production are antimicrobial mechanisms associated with NADPH oxidase. This study evaluated the effects of g.18174 A > G and g.18270C > T single-nucleotide polymorphisms (SNP) in NCF4 on bovine mastitis and the respiratory burst capacity of neutrophils. SNPs of 160 dairy cattle were determined using a novel PCR-RFLP protocol by employing restriction enzymes, MboI and FokI. Also, the flow cytometry measured respiratory burst in 82 blood samples. Our results indicated that only g.18174 A > G SNP reduced the respiratory burst capacity. However, both SNPs were not significantly correlated with clinical mastitis. We concluded that g.18174 A > G decreases the function of NADPH oxidase. However, both SNPs were not significantly correlated with clinical mastitis.

Destination of corpus luteum in postpartum clinical endometritis cows and factors affecting self-recovery.

In this study, the fate of corpus luteum was investigated in cows affected by severe clinical endometritis. Also, concentration of IL-1ß, IL-6, COX-2, adiponectin, leptin, progesterone (P4), PGFM, insulin, and IGF-1 were studied in severe clinical endometritis cows at day 30 ± 3 postpartum. Eighty-seven dairy cows affected by severe clinical endometritis were selected and their reproductive tract was examined by ultrasonography at day 30 ± 3 postpartum (first examination) and 14 days later (second examination). The majority of the cows with CL and affected by clinical endometritis at first examination had new CL 14 days later, and most of these cows were clean without any treatment (self-healing). The CL of about 28.7% of all cows with clinical endometritis at first examination persisted two weeks later and the uterus of most of them remained infected as pyometric cows. Most of the anestrous cows (83.3%) were pregnant, but only 51% of cows with new CL were pregnant at the end of next six month period. There was a significant difference in the means of lactation number, uterine lumen diameter, cervical diameter, and size of uterine horn, milk production at first examination, concentrations of insulin, COX-2, P4, IL-1ß, and IL-6 among persistent CL, new CL, and anestrous cows. The pregnancy rate and the discharge score were different among persistent CL, new CL, and anestrous cows. In conclusion, CL on ovary of cows and its fate could affect recovery from severe clinical endometritis. The concentration of some metabolic hormones influenced the self-recovery of cows from clinical endometritis.

Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages.

This study assessed the changes of plasma vitamin A, E, and C and the lipid peroxidation status of sheep during breeding and pregnancy under drought conditions. The study was conducted on 105 cross-bred fat tailed ewes, 3-5 years old with body condition scores (BCS) of 2.5 to 3.5. The ewes were grazing on medium-to-low quality forages during summer and low quality forages within the succeeding months and had ad libitum access to a mixture of alfalfa hay (40%) and wheat straw (60%) in the afternoons. From 3 weeks before breeding till 1 month after the introduction of rams, 300 g of barley grain/head/day was offered to the ewes and then the supplemental grain was reduced to 100 g/head/day. For better synchronization of estrus cycles in ewes, they were isolated from the rams for at least 2 months and then kept in close proximity of the rams for 1 week before the introduction of the rams to the ewe flock. Then, whole blood samples were collected on days 1, 7, 21, and 120 after ram introduction. Vitamins A, E, and C were measured in plasma. Malondialdehyde (MDA) was measured in the hemolysate as a biomarker of lipid peroxidation. Plasma progesterone (P4) was measured in the samples of day 120 for assessing pregnancy status of the ewes. Vitamins A and C showed continuous and significant declines (P < 0.05) through days 1 to 120. Vitamin E declined only during the first 21 days of the study and remained almost constant till day 120. MDA concentration increased significantly at day 21. An elevated concentration of MDA was also detected at day 120. The difference between days 21 and 120 was not significant (P > 0.05). A positive correlation between vitamins E and C was detected at day 120 (r = 0.349, P < 0.01). Age and BCS did not affect the patterns of changes. Assuming that the ewes with P4 concentrations ≥2.5 ng/ml were pregnant, 95 out of 105 ewes (90.5%) were pregnant at day 120 of the study. Under the conditions of the present study with medium-to-low quality pastures as the main sources of feed, ewes of various ages and body conditions may suffer from oxidative stress during breeding and pregnancy.