The Genie Is Out of the Bottle: What ChatGPT Can and Cannot Do for Medical Professionals.

ChatGPT is a cutting-edge artificial intelligence technology that was released for public use in November 2022. Its rapid adoption has raised questions about capabilities, limitations, and risks. This article presents an overview of ChatGPT, and it highlights the current state of this technology for the medical field. The article seeks to provide a balanced perspective on what the model can and cannot do in three specific domains: clinical practice, research, and medical education. It also provides suggestions on how to optimize the use of this tool.

Development of a chemically-defined minimal medium for studies on growth and protein uptake of Gemmata obscuriglobus.

We experimentally determined minimal media requirements for Gemmata obscuriglobus, a Gram-negative Planctomycete bacteria with several unusual physiological features. We find that supplementing media with the usual vitamins solution does not improve viability, but does result in an increased growth rate in liquid cultures and a larger colony size on agar plates. By systematically including individual vitamins, or omitting individual vitamins, from media we find that the addition of only two vitamins, biotin and cyanocobalamin, are sufficient to restore colony growth to comparable rates as other commonly used media. Overall, our findings define minimal media requirements for the culturing of this low-nutrient organism. One of G. obscuriglobus unusual physiological features is the ability to internalize fully-folded proteins. Using fluorescence microscopy and flow cytometery we show that this physiological behavior is dependent on media state and composition. The percentage of cells exhibiting internalization of GFP when grown on a particular, solid minimal medium is far greater than cells grown in liquid medium of similar composition or other solid media with different compositions.