Subject(s)
Metabolism , Nutritional Physiological Phenomena , Bariatric Surgery , Diabetes Mellitus, Type 2/diet therapy , Diabetes Mellitus, Type 2/metabolism , Diabetes Mellitus, Type 2/physiopathology , Humans , Obesity, Morbid/metabolism , Obesity, Morbid/physiopathology , Obesity, Morbid/surgeryABSTRACT
Glycine betaine relieved sodium chloride-mediated inhibition of growth in Azospirillum lipoferum ATCC 29708. 35S-methionine labelling of proteins after salinity up-shock revealed strong induction of a 30 kDa protein which cross-reacted with the anti-glycine betaine binding protein antibody from Escherichia coli. This suggested that A. lipoferum had a salinity-induced ProU-like high-affinity glycine betaine transport system. A genomic library of A. lipoferum ATCC 29708 was screened for the proU-like gene by complementation of a proU mutant of E. coli. Four recombinant cosmids, capable of restoring growth of the proU mutant on plates containing 600 mM NaCl and 1 mM glycine betaine were selected. Selected recombinant cosmids hybridized with a proU gene probe from E. coli. Complementation of E. coli proU mutant with the A. lipoferum genomic DNA was evident by the ability of proU mutant (containing selected recombinant cosmids) to grow on minimal medium supplemented with 600 mM NaCl and 1 mM glycine betaine.
Subject(s)
Amino Acid Transport Systems , Azospirillum/genetics , Bacterial Proteins/genetics , Carrier Proteins/genetics , Escherichia coli/genetics , Genes, Bacterial/genetics , Azospirillum/growth & development , Blotting, Southern , Blotting, Western , Electrophoresis, Agar Gel , Escherichia coli/growth & development , Genetic Complementation Test , Time FactorsSubject(s)
Behcet Syndrome/complications , Headache/etiology , Pseudotumor Cerebri/etiology , Adult , Behcet Syndrome/diagnosis , Female , HumansABSTRACT
A DNA probe containing the structural gene for dicarboxylate transport (dct A) of Rhizobium meliloti hybridized strongly with the fragments of Azospirillum lipoferum genomic DNA. A genomic library of A. lipoferum was screened for the dct A gene by complementation of a dct A mutant of Rhizobium meliloti. A recombinant cosmid, p37D, capable of restoring growth of the dct A mutant on dicarboxylates was isolated and found to hybridize to the dctA probe. The ability of p37D to complement the dct B mutant of R. meloliti indicated that dct A and dct B genes in A. lipoferum may be organized adjacent to each other.
Subject(s)
Azospirillum/genetics , Bacterial Proteins , Dicarboxylic Acid Transporters , Sinorhizobium meliloti/genetics , Biological Transport, Active/genetics , Carrier Proteins/genetics , Carrier Proteins/metabolism , Cloning, Molecular , Cosmids , DNA Probes , DNA, Bacterial/genetics , Dicarboxylic Acids/metabolism , Genes, Bacterial , Genetic Complementation Test , Multigene Family , Mutation , Protein Kinases/genetics , Protein Kinases/metabolism , Receptors, Cell Surface/genetics , Receptors, Cell Surface/metabolism , Restriction Mapping , Sinorhizobium meliloti/metabolismABSTRACT
One hundred and ninety-two patients with peripheral lymphadenopathy were screened and 80 patients with tubercular lymphadenitis were studied. Their ages ranged from 1 to 65 years; most were younger than 30 years and there was a slight female preponderance (1.2:1). Seventy per cent of patients were of low socioeconomic status. Of the 80 patients, 56 had affected cervical nodes, seven had inguinal nodes, five had axillary nodes and 12 had multiple sites of lymph node involvement. All had enlarged nodes which were matted in 44 cases and discrete in 18 cases, while the rest had either an abscess or a discharging sinus. Fifty-nine cases (74 per cent) showed a positive Mantoux test and four cases (5 per cent) had associated pulmonary tuberculosis. Fine needle aspiration cytology gave a positive diagnosis in 66 cases (83 per cent). Fifty-two cases showed a positive culture for Mycobacterium tuberculosis of human type in Lowenstein-Jensen medium. Short-term chemotherapy (9 months) consisting of rifampicin, isoniazid and ethambutol gave an excellent result. Surgery was not required in any of the cases.
