Rosetteness in Portulaca grandiflora: an altered genetic expression.

Rosetteness is either developmentally controlled or induced by various biotic and abiotic stresses. Prolonged vegetative growth and/or pruning seems to be inducing rosetteness in Portulaca grandiflora. Analysis of cellular extracts from rosette stems by SDS-PAGE, revealed induction of a polypeptide of high molecular mass (approximately equal to 58 kDa) and over-expression of a few lower molecular weight polypeptides. However, leaves showed no differences in the protein profiles.

Clinico-therapeutic profile of pyogenic liver abscess.

Nineteen consecutive patients of pyogenic liver abscess (13 males, 6 females, mean age 45.2 +/- 6, 5 years) were studied over a period of 5 years for the clinical profile and therapeutic efficacy of percutaneous aspiration. Majority of them presented with spiking fever (94.7%), pain over right upper quadrant of abdomen (53.8%) and often with prostration and shock (31.6%). The onset has been rather acute in patients with multiple abscesses (7 cases). Ascites (10.5%), clubbing of fingers (15.8%) and splenomegaly (10.5%) were observed in cases with long duration of illness. There has been the polymorphonuclear leucocytosis (89.5%) and mild to moderate anaemia (52.6%). Jaundice (42.1%) was usually mild degree (serum bilirubin 4.6 +/- 2.4 mg/dl). The serum transaminases and alkaline phosphatase were raised in 94.9% of cases. Ultrasonography revealed predominantly hypoechoic (54.1%) areas with frequent distal acoustic enhancement and internal echoes (21.6%). Seven patients had multiple abscesses with 25 lesions (size 5.2 +/- 4.6 cms) situated mainly over antero-inferior segment of the right lobe (45.9%) of the liver. The blood and pus cultures demonstrated the micro-organisms (positive in 63.9% and 86.6% respectively) predominantly of bowel flora including facultative gram negative rods and anaerobes. Mortality was 26.5% and pleuro-pulmonary complications were the commonest (26.4%) one. 15 cases were treated by percutaneous aspiration, proper antibiotic and metronidazole with encouraging results (only two deaths). Complete resolution of abscess took place in 14.2 +/- 1.2 weeks (range 6 weeks to 7.5 months). It is emphasized that percutaneous needle aspiration is useful both for diagnosis and treatment of pyogenic liver abscess.

Quantitative immunological status of males of unexplained infertility in eastern U.P.

126 males of unexplained infertility and 75 fertile males were studied during a period of more than 2 years. Statistically significant increase in the levels of serum IgM was observed in cases of males of unexplained infertility. Level and percentage of cases showing semen IgM was more in study group (134.61 +/- 25.5 mg %, 41.26%) than control (24%, 99.3 +/- 8.3%). Semen IgA was found only in study group in 30.15% cases. Though definite correlation between serum and semen immunoglobulin was not observed, semen immunoglobulin was found to be 1-2 fold lower than serum. Results of the present study indicate towards a possible immunological role in cases of unexplained infertility in males.

Male gonads in leprosy--a clinico-pathological study.

Sixty male leprosy patients (mean age 27.2 +/- 5.04 years) selected at random, were studied for gonadal involvement with the mean duration of illness 4.17 +/- 3.27 years. Only lerpomatous and borderline leprosy cases developed testicular and epididymal changes. Testicular pain and/or swelling (lepromatous 62.5%, borderline 30%) was the main presenting feature. Altered sexual function was observed in 34(56.6%) cases, and 11 patients revealed altered sexual hair pattern. Gynecomastia was seen in 9 cases. Reduced testicular size along with its soft feeling was present in 25% of cases while no testicular sensation was felt in 8 (13.3%) cases, and impaired testicular sensation in 9 (15%) of them. Spermogram revealed azoospermia in 19 (35%) and oligospermia in 16 (26.6%) cases. Histo-pathology revealed evidences of leprous pathology irrespective of testicular size, semen picture and clinical manifestations. There was marked variation in histopathological findings in testes and hence it was difficult to categorise them into vascular, interstitial and obliterative phase.

Glucosamine-resistant mutations in yeast affecting the glucose repression sensitivity of electron transport enzymes.

We have isolated two mutant strains, GSA(r)-4 and GSA(r)-5, which are able to grow on lactate in the presence of D-glucosamine. The glucosamine-resistant phenotype results from the cooperative effects of mutations in three loci, GAR1, GAR2 and GAR3. Both glucosamine resistant mutant strains were doubly mutant at gar1 gar2 (GSA(r)-4) or gar1 gar3 (GSA(r)-5). The mutants were also shown to exhibit glucose repression insensitive synthesis of NADH-cytochrome c reductase and cytochrome c oxidase. Glucose-repressible synthesis of the following enzymes was seen: succinic dehydrogenase, succinic: cytochrome c reductase, maltase (PNPGase), galactokinase, α-galactokinase. The glucose-repression insensitivity segregates in association with the glucosamine resistance.

Role of Petiole in Protein Metabolism of Senescing Betel (Piper betle L.) Leaves.

Effects of depetiolation on protein metabolism during senescence of detached betel (Piper betle L.) leaves have been studied. In normal petiolated leaves, the level of chlorophyll and proteins and extent of protein synthesis declined, while the protease activity registered manifold increase with the advancement of senescence. All of these changes were delayed by depetiolation/de-midribbing treatments, though without affecting the general pattern of senescence. Thus, the presence of petiole seems to expedite protein degradation, probably due to earlier attainment of optimal concentration of proposed senescence factor(s) (Mishra and Gaur 1970 Science 167: 387).

Primary liposarcoma of the mastoid.

A unique case of liposarcoma of the mastoid in a 4-year-old child presenting as acute mastoiditis and subperiosteal abscess has been reported.

Modified conservative treatment of club foot.

In 120 cases of severe, neglected or recurrent club foot, a modified conservative method of treatment has yielded encouraging results. The method consists of blind calcaneal fasciotomy, repositioning of the foot in a corrected position and plaster cast application from the upper thigh to the toes. Nineteen recurrences in this series were treated by Dwyer's calcaneal osteotomy, or medial soft tissue release and/or tendon transposition.