ABSTRACT
Effects of disodium salt 2,4-di(1-metoxyethyl)-deuteroporphyrine-IX (Dimegin) and the light from Soret band (¼395-405 nm) at the viability of microbial cells and at their potential to form microbial biofilms have been compared with traditional antiseptics. Irradiation of microbial cells of S. aureus, E. coli, C. albicans and others with diode light (power density 0.05 Wt/cm2) caused a bactericidial effect similar to that obtained with standard anticeptics (chlorhexidine and dioxidine). A comparative study of the effectiveness of Dimegin and Photoditazine (a soluble salt of chlorine e6) as photosensitizers have been performed using the test system of erythrocyte hemolysis in vitro under irradiation with light from the Sore band. Results have shown insignificant difference in the photodynamic effect with similar doses of absorbed light and preparation concentration.
Subject(s)
Deuteroporphyrins/pharmacology , Photosensitizing Agents/pharmacology , Candida albicans/drug effects , Candida albicans/growth & development , Chlorhexidine/pharmacology , Disinfectants/pharmacology , Escherichia coli/drug effects , Escherichia coli/growth & development , Glucosamine/analogs & derivatives , Glucosamine/pharmacology , Humans , Light , Microbial Sensitivity Tests , Microbial Viability/drug effects , Photochemotherapy , Quinoxalines/pharmacology , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & developmentABSTRACT
New biomarkers of effect of some controlled ambient air pollutants (formaldehyde, carbon monoxide and TSP) were found in two surveys of Moscow residents (apparently healthy and outpatients with allergic diseases) with a help of screening system of biochemical and immunological parameters. Possible mechanisms of the effects, including interference of atmospheric carbon monoxide and sulfur dioxide with corresponding endogenous second messengers, are discussed.
Subject(s)
Biomarkers/analysis , Environmental Illness/epidemiology , Immunity , Particulate Matter/adverse effects , Population Surveillance/methods , Public Health , Urban Population , Adult , Environmental Health , Environmental Illness/diagnosis , Environmental Illness/metabolism , Female , Humans , Incidence , Male , Middle Aged , Moscow/epidemiology , Retrospective Studies , Young AdultABSTRACT
Factor analysis of cross-sensitization to 24 common allergens and correlation analysis of the relationship between the hypersensitivity to the allergens and some biochemical markers (the intensity of serum chemiluminescence, the content of SH-groups, and the activity of lysosomal enzymes) of the health status were carried out in a sample of Moscow residents with allergic diseases. A significant correlation was found between the serum levels of specific IgE antibodies to pollen allergens and the activity of serum acid DNAase (R = 0.498; p = 0.009; N = 67). The revealed regularity may be used to devise a test for the differential diagnosis of pollenoses and suggests that there are possible differences in the levels of apoptosis and cytogenetic damages between patients with pollenoses and those with other allergic diseases.
Subject(s)
Allergens/immunology , Deoxyribonucleases/blood , Hypersensitivity/enzymology , Immunoglobulin E/blood , Pollen/immunology , Adolescent , Adult , Aged , Female , Humans , Hypersensitivity/blood , Male , Middle Aged , Moscow , Regression Analysis , Young AdultABSTRACT
The immune status of Moscow dwellers was studied. The findings indicate the criterion significance of immunological and allergological parameters in the estimation of a risk of allergic diseases under the influence of poor environmental factors influence. Hyperactivity in individuals with high immunoglobulin class E levels to environmental factors is suggested by the elevated concentrations of serum specific IgE to intrahouse factors that in combination with the increased levels of anti-inflammatory cytokines (interferon-gamma, tumor necrosis factor-alpha) and the suppressed production interleukin-4 are criteria for the early manifestations of allergy.
