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1.
J Atheroscler Thromb ; 29(2): 152-173, 2022 Feb 01.
Article in English | MEDLINE | ID: mdl-33298663

ABSTRACT

AIM: The Dietary Approaches to Stop Hypertension (DASH) diet is recommended for lowering blood pressure (BP). Our previous single-arm trial revealed that the Japanese cuisine-based DASH (J-DASH) diet (supplying NaCl 8.0 g per day) reduced BP and improved cardiometabolic biomarkers. The present study's primary objective was to test the feasibility of the J-DASH diet based on its effects on the BP and BP variability of subjects with untreated high-normal BP or stage 1 hypertension. METHODS: The 6-month study period was held from December 2015 to August 2016. The participants were recruited through advertisements in local newspapers and our website and from among randomized participants at Yamaguchi University Hospital. The 2-month treatments included the following: the J-DASH-1 diet 1×/day or the J-DASH-2 diet providing a fish hamburger-patty 2×/day (5 days/week respectively). The control group consumed their usual diets. For the subsequent 4 months, all participants consumed their usual diets. The main outcome measure was the feasibility of the J-DASH diet. We also collected the data of clinic BP and home BP (by automatic BP monitor), cardiometabolic biomarkers, and lifestyle and psychosocial parameters during the intervention phase. We examined behavior changes throughout the study period, and the diets' safety. RESULTS: Fifty-one participants were recruited; following screening, 48 met the inclusion criteria and were randomized by central allocation. Eight participants were eliminated based on exclusion criteria, and the 40 participants were randomly allocated to the J-DASH 1 and J-DASH 2 groups ( n=13 each) and the usual-diet group (n=14). The participants' mean age was 50 years, and 44% were women. The three groups' clinic BP values were not significantly different, but the home BP values were lower in the J-DASH 1 group and lowest in the J-DASH 2 group compared to the usual-diet group and differed significantly among the three groups throughout the study period (p<0.0001). The home BP variability was significantly lower in the J-DASH groups compared to the usual-diet group throughout the study period ( p<0.01). The other indices including fish oil showed little differences among the groups throughout the study period. CONCLUSIONS: The J-DASH diet was feasible to improve home BP and stabilize its variability, and it did so more effectively than the participants' usual diets.


Subject(s)
Dietary Approaches To Stop Hypertension , Fish Oils/therapeutic use , Hypertension/diet therapy , Aged , Cohort Studies , Feasibility Studies , Female , Humans , Hypertension/diagnosis , Japan , Life Style , Male , Middle Aged
2.
Curr Hypertens Rev ; 16(2): 128-137, 2020.
Article in English | MEDLINE | ID: mdl-30887927

ABSTRACT

BACKGROUND: We previously reported the nutritional characteristics and effects of the DASH-JUMP diet, which is a WASHOKU-modified DASH diet, in Japanese participants with untreated high-normal blood pressure or stage 1 hypertension. The dietary adherence of the DASH diet in Japanese participants has never been evaluated before. OBJECTIVE: We aimed to assess the relationships between dietary adherence, self-efficacy, and health behavior change among study participants who received the DASH-JUMP diet by home delivery. METHODS: Participants were treated with the DASH-JUMP diet for 2 months and consumed their usual diets for the next 4 months. We conducted surveys using the stage of behavior change model questionnaire and the modified perceived health competence scale Japanese version questionnaire at baseline and 1, 2, 3, and 6 months to assess dietary adherence. RESULTS: Forty-three participants (25 men, 18 women; mean age 53.6 ± 8.2 years) returned completed questionnaires, which we analyzed. Health behavior change was motivated by previous behavioral changes and improved biomarkers. The improvement and maintenance of self-efficacy were deeply related to health behavior change and previous self-efficacy. The experience of the DASH-JUMP study for participants included three processes to improve lifestyle habits: Phase 1, reflecting on previous lifestyle habits; Phase 2, learning through new experiences and the acquisition of knowledge; and Phase 3, desiring to maintain their own health. CONCLUSION: It indicated that the DASH-JUMP diet significantly increased self-efficacy and promoted health behavior change.


Subject(s)
Diet, Healthy , Dietary Approaches To Stop Hypertension , Feeding Behavior , Health Knowledge, Attitudes, Practice , Hypertension/diet therapy , Patient Compliance , Risk Reduction Behavior , Self Efficacy , Biomarkers/blood , Blood Pressure , Female , Health Status , Humans , Hypertension/diagnosis , Hypertension/physiopathology , Hypertension/psychology , Japan , Male , Middle Aged , Time Factors , Treatment Outcome , Weight Loss
3.
Curr Hypertens Rev ; 14(1): 56-65, 2018.
Article in English | MEDLINE | ID: mdl-29618312

ABSTRACT

BACKGROUND: We developed a WASHOKU-modified DASH diet named DASH-JUMP. We previously reported the hypotensive effect of the DASH-JUMP diet in Japanese participants with untreated high-normal Blood Pressure (BP) or stage 1 hypertension. OBJECTIVE: We aim to introduce the DASH-JUMP diet worldwide as a new lifestyle medicine. Accordingly, we prospectively assessed the nutritional characteristics of the DASH-JUMP diet. METHODS: Participants were treated with the DASH-JUMP diet for 2 months. Then, for 4 months after the intervention, they consumed their usual diets. We conducted a nutritional survey using the FFQg nutrient questionnaire at baseline and after 1, 2, 3, and 6 months. We received completed questionnaires from 55 participants (28 men and 27 women; mean age 54.2 ± 8.0 years) and analyzed them. RESULTS: The DASH-JUMP diet is rich in green-yellow vegetables, seaweed, milk, and mushrooms, while it has low contents of meat, eggs, confectionery, oils and fats, pickles, shellfish boiled in sweetened soy sauce, and fruits. Nutrients significantly associated with the observed change in systolic BP were niacin (P = 0.005) and carbohydrate (P = 0.033). The results of the FFQg questionnaire revealed that participants who had an increased BP at 1 month after ceasing the intervention had eating habits that broadly imitated the DASH-JUMP diet at 4 months after ceasing the intervention. Therefore, the systolic and diastolic BP values at 4 months after ceasing the intervention decreased significantly compared to those at baseline. CONCLUSION: The DASH-JUMP diet may represent a new lifestyle medicine for reducing hypertension.


Subject(s)
Blood Pressure , Diet, Healthy , Dietary Approaches To Stop Hypertension/methods , Hypertension/diet therapy , Nutritive Value , Adult , Aged , Feeding Behavior , Female , Humans , Hypertension/diagnosis , Hypertension/physiopathology , Japan , Male , Middle Aged , Prospective Studies , Recommended Dietary Allowances , Time Factors , Treatment Outcome
4.
Nutrients ; 9(2)2017 Feb 13.
Article in English | MEDLINE | ID: mdl-28208812

ABSTRACT

Several studies have reported that short sleep duration is a risk factor for obesity and metabolic disease. Moreover, both sleep duration and sleep timing might independently be associated with dietary nutrient intake. In this study, we investigated the associations between self-reported sleep duration and dietary nutrient intake, with and without adjustments for variations in sleep timing (i.e., the midpoint of sleep). We conducted a questionnaire survey, comprising a validated brief self-administered diet history questionnaire (BDHQ) and the Japanese version of the Pittsburgh Sleep Quality Index (PSQI) among 1902 healthy Japanese adults and found that the dietary intakes of several nutrients correlated with sleep duration among men regardless of adjustment for the midpoint of sleep. Particularly, (1) small but significant correlations were observed between sleep duration and the percentage of energy from protein, regardless of adjustment for the midpoint of sleep; (2) energy-adjusted intakes of sodium, vitamin D, and vitamin B12 also significantly correlated with sleep duration; and (3) intakes of bread, pulses, and fish and shellfish correlated with sleep duration. In contrast, no significant correlations were observed between sleep duration and dietary intakes among women. This study revealed that after controlling for the midpoint of sleep, sleep duration correlated significantly with the dietary intake of specific nutrients and foods in a population of Japanese men.


Subject(s)
Diet , Self Report , Sleep , Adult , Asian People , Body Mass Index , Cross-Sectional Studies , Dietary Carbohydrates/administration & dosage , Dietary Fats/administration & dosage , Dietary Proteins/administration & dosage , Female , Humans , Japan , Male , Micronutrients/administration & dosage , Middle Aged , Multivariate Analysis , Nutrition Assessment , Regression Analysis , Surveys and Questionnaires
5.
Mol Nutr Food Res ; 61(5)2017 05.
Article in English | MEDLINE | ID: mdl-28019085

ABSTRACT

SCOPE: Zinc is an essential mineral that plays an important role in the body. We previously reported that orally feeding zinc-enriched yeast to mice induces nonrapid-eye-movement sleep. In addition, astaxanthin, an antioxidant abundant in seafood such as salmon and krill, is able to chelate minerals and may promote zinc absorption, which in return may also improve sleep. The purpose of our study was to examine the effect of zinc-rich and astaxanthin-containing food on sleep in humans. METHODS AND RESULTS: We conducted a randomized, double-blinded, placebo-controlled parallel group trial of 120 healthy subjects and recorded their night activity by actigraphy for 12 weeks. These subjects were divided into four groups: placebo, zinc-rich food, zinc-, and astaxanthin-rich food, and placebo supplemented with zinc-enriched yeast and astaxanthin oil. Compared with the placebo group, the zinc-rich food group efficiently decreased the time necessary to fall asleep and improved sleep efficiency, whereas the group that ingested zinc-enriched yeast and astaxanthin oil significantly improved the sleep onset latency. CONCLUSION: Actigraphic sleep monitoring demonstrated that eating zinc-rich food improved sleep onset latency as well as improved the sleep efficiency in healthy individuals.


Subject(s)
Food, Fortified , Ostreidae/chemistry , Saccharomyces cerevisiae , Seafood/analysis , Sleep/drug effects , Zinc/pharmacology , Actigraphy , Adult , Aged , Aged, 80 and over , Animals , Antioxidants/pharmacology , Cholesterol/blood , Diet , Diet Records , Dietary Supplements , Double-Blind Method , Female , Humans , Male , Middle Aged , Surveys and Questionnaires , Xanthophylls/pharmacology , Young Adult , Zinc/blood
6.
FEMS Yeast Res ; 17(1)2017 02.
Article in English | MEDLINE | ID: mdl-27915244

ABSTRACT

Protamine peptide (PP) derived from salmon is a 14-mer with 10 arginine residues. We investigated the in vitro and in vivo antifungal activity of PP against Candida albicans PP showed a concentration-dependent dual mode of action, with fungicidal activity and inhibitory activity for hyphal development in vitro. At lethal concentrations of PP, intracellular accumulation of PP was energy-dependent but independent of endocytosis, and resulted in ATP efflux and the generation of reactive oxygen species in the cells. PP at sublethal concentrations inhibited hyphal development in C. albicans by binding to the cell surface. Though antifungal activity of PP was inactivated by high concentrations of NaCl, the antifungal activity of the synthetic cyclic (via a disulfide bond) form of PP (cyclic PP) was not. Cyclic PP also showed the concentration-dependent dual mode of action, and had five-fold greater antifungal activity than PP. The advantage of antifungal activity of cyclic PP compared with PP in vitro resulted in a high in vivo efficacy in a murine oral candidiasis model. Oral treatment with cyclic PP inhibited hyphal development of C. albicans on mouse tongues and protected against the development of severe candidiasis. This study shows the therapeutic potential of cyclic PP as an antifungal peptide against C. albicans.


Subject(s)
Antifungal Agents/metabolism , Candida albicans/drug effects , Heparin Antagonists/metabolism , Peptides, Cyclic/metabolism , Protamines/metabolism , Adenosine Triphosphate/metabolism , Animals , Antifungal Agents/therapeutic use , Candida albicans/growth & development , Candida albicans/physiology , Candidiasis, Oral/drug therapy , Disease Models, Animal , Heparin Antagonists/therapeutic use , Hyphae/drug effects , Hyphae/growth & development , Mice , Microbial Viability/drug effects , Peptides, Cyclic/therapeutic use , Protamines/therapeutic use , Reactive Oxygen Species/metabolism , Salmon , Treatment Outcome
7.
Hypertens Res ; 39(11): 777-785, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27412796

ABSTRACT

The Dietary Approaches to Stop Hypertension (DASH) diet is recommended by the American Heart Association to lower blood pressure (BP); however, its effects in Japanese participants have not been rigorously studied. We assessed the effects of the DASH-Japan Ube Modified diet Program (DASH-JUMP), a modified DASH diet, on cardiometabolic and inflammatory biomarkers in Japanese participants with untreated high-normal BP or stage 1 hypertension. Fifty-eight participants (30 men and 28 women; mean age 54.1±8.1 years) with untreated high-normal BP or stage 1 hypertension followed the DASH-JUMP (salt 8.0 g per day) for 2 months. After the intervention period, they resumed their usual diets for 4 months. The DASH-JUMP significantly decreased the participants' body mass index values (24.6±3.5 kg m-2 at baseline23.2±3.3 kg m-2 at 2 months, P=0.000), BP (153±14/91±11 mm Hg at baseline130±16/80±9 mm Hg at 2 months, P=0.000 and 139±16/85±10 mm Hg at 6 months, P=0.000), fasting serum glucose level (100±26 mg dl-194±15 mg dl-1 at 2 months, P=0.003) and fasting insulin level (6.9±5.9 µIU ml-14.4±2.7 µIU ml-1 at 2 months, P=0.000). The mean compliance of the participants for the DASH-JUMP diet was 88.5%. The DASH-JUMP diet reduced cardiovascular risk factors and may be an effective nutritional strategy for preventing cardiovascular events.


Subject(s)
Blood Pressure/physiology , Body Mass Index , Hypertension/diet therapy , Blood Glucose , Female , Health Behavior , Humans , Hypertension/blood , Insulin/blood , Life Style , Male , Middle Aged , Patient Compliance , Risk Factors , Treatment Outcome
8.
Gene ; 516(2): 228-37, 2013 Mar 10.
Article in English | MEDLINE | ID: mdl-23266625

ABSTRACT

Protamine is a small, arginine-rich, nuclear protein that replaces histone late in the haploid phase of spermatogenesis and is believed to be essential for sperm head condensation and DNA stabilization. Protamine has many biological activities and has roles in hematopoiesis, immune responses, the nervous system and bone metabolism. Bone sialoprotein (BSP) is a mineralized connective tissue-specific protein expressed in differentiated osteoblasts that appears to function in the initial mineralization of bone. Protamine (71.35 ng/ml) increased BSP mRNA levels by 6h in osteoblast-like ROS 17/2.8 cells. In a transient transfection assay, protamine (71.35 ng/ml) increased luciferase activity of the construct (-116 to +60) in ROS 17/2.8 cells and rat bone marrow stromal cells. Luciferase activities induced by protamine were blocked by protein kinase A, tyrosine kinase and ERK1/2 inhibitors. Introduction of 2 bp mutations to the luciferase constructs showed that the effects of protamine were mediated by a cAMP response element (CRE), a fibroblast growth factor 2 response element (FRE) and a homeodomain protein-binding site (HOX). Gel shift analyses showed that protamine (71.35 ng/ml) increased the nuclear protein binding to CRE, FRE and HOX. CREB, phospho-CREB, c-Fos, c-Jun, JunD and Fra2 antibodies disrupted the formation of CRE-protein complexes. Dlx5, Msx2, Runx2 and Smad1 antibodies disrupted FRE- and HOX-protein complex formations. These studies demonstrate that protamine induces BSP transcription by targeting CRE, FRE and HOX sites in the proximal promoter of the rat BSP gene. Moreover, phospho-CREB, c-Fos, c-Jun, JunD, Fra2, Dlx5, Msx2, Runx2 and Smadl transcription factors appear to be key regulators of protamine effects on BSP transcription.


Subject(s)
Gene Expression/drug effects , Integrin-Binding Sialoprotein/genetics , Protamines/pharmacology , Animals , Base Sequence , Cells, Cultured , Chromatin Immunoprecipitation , Core Binding Factor Alpha 1 Subunit/genetics , Core Binding Factor Alpha 1 Subunit/metabolism , Genes, Reporter , Integrin-Binding Sialoprotein/metabolism , Luciferases/genetics , Luciferases/metabolism , Molecular Sequence Data , Promoter Regions, Genetic , Rats , Transfection
9.
J Mater Sci Mater Med ; 22(12): 2607-15, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21922330

ABSTRACT

A DNA/protamine complex powder was prepared by reaction between DNA and protamine sulfate solution with stirring in order to develop a new injectable biomaterials for dental therapy. The powder of DNA/protamine complex became paste by kneading the complex powder and distilled water. Complex formation was confirmed by FT-IR measurement. The complex paste had a porous structure and its viscosity was approximately 280.1 Pas. The paste could easily pass through a needle of 0.25 mm internal diameter. It seemed that DNA/protamine complex paste has suitable viscosity for clinical use as an injectable biomaterial. Although, the complex paste delayed the growth speed of Staphylococcus aureus, Pseudomonas aeruginosa, Porphyromonas gingivalis and Prevotella intermedia for limited periods, it cannot kill and inhibit growing bacteria. The complex paste disk showed a mild tissue response and gradually degraded after the implantation into the soft tissue of rats. These results suggested that this DNA/protamine complex paste could be a useful material for a biodegradable biomaterial. In particular, this paste will be applicable as an injectable biomaterial using syringe for the repair of defects of living tissue, GBR treatment and/or GTR treatment in dentistry.


Subject(s)
Biocompatible Materials/administration & dosage , DNA/chemistry , Dental Materials/chemistry , Protamines/chemistry , Animals , Anti-Bacterial Agents/chemistry , Male , Microbial Sensitivity Tests , Microscopy, Electron, Scanning , Porosity , Porphyromonas gingivalis , Powders , Prevotella intermedia , Pseudomonas aeruginosa , Rats , Rats, Sprague-Dawley , Spectrophotometry, Infrared , Spectroscopy, Fourier Transform Infrared , Staphylococcus aureus , Viscosity , Water/chemistry
10.
Dent Mater J ; 29(5): 529-35, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20733264

ABSTRACT

The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.


Subject(s)
Biocompatible Materials/chemical synthesis , DNA/chemical synthesis , Protamines/chemical synthesis , 3T3 Cells , Animals , Anti-Bacterial Agents/pharmacology , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Cell Survival/drug effects , DNA/chemistry , DNA/pharmacology , DNA Adducts/chemical synthesis , DNA Adducts/chemistry , DNA Adducts/pharmacology , Implants, Experimental , Male , Materials Testing , Mice , Microbial Sensitivity Tests , Microscopy, Electron, Scanning , Osteoblasts/drug effects , Porosity , Porphyromonas gingivalis/drug effects , Prevotella intermedia/drug effects , Protamines/chemistry , Protamines/pharmacology , Rats , Rats, Sprague-Dawley , Salmon , Solubility , Staphylococcus aureus/drug effects , Subcutaneous Tissue/drug effects , Subcutaneous Tissue/pathology , Surface Properties , Water
11.
Biotechnol Bioeng ; 83(4): 474-8, 2003 Aug 20.
Article in English | MEDLINE | ID: mdl-12800141

ABSTRACT

Acetobacter xylinum BPR2001 produces water-insoluble bacterial cellulose (BC) and a water-soluble polysaccharide called acetan in corn steep liquor-fructose medium. Acetobacter xylinum EP1, which is incapable of acetan production was derived by disrupting the aceA gene of BPR2001. The BC production by EP1 (2.88 g/L) was lower than that by BPR2001 (4.6 g/L) in baffled-flask culture. When purified acetan or agar was added to the medium from the start of cultivation, the BC production by EP1 was enhanced and the final BC yield of EP1 was almost the same as that of BPR2001. A similar improvement of BC production by EP1 by the addition of agar was also confirmed by cultivation in a 50-L airlift reactor. From these results, the role of acetan in BC production is associated with the increase in the viscosity of the culture medium which may hinder coagulation of BC and cells in the culture, thereby accelerating the growth of BPR2001 and BC production by BPR2001.


Subject(s)
Cellulose/biosynthesis , Fructose/metabolism , Genetic Enhancement/methods , Gluconacetobacter xylinus/growth & development , Gluconacetobacter xylinus/metabolism , Polysaccharides, Bacterial/metabolism , Water/chemistry , Cellulose/ultrastructure , Gluconacetobacter xylinus/classification , Gluconacetobacter xylinus/genetics , Mutagenesis, Site-Directed , Polysaccharides, Bacterial/chemistry , Polysaccharides, Bacterial/genetics , Solubility , Species Specificity
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