ABSTRACT
Rotavirus infection is associated with acute infantile gastroenteritis in infants and young children globally. In South Africa, rotavirus infection has been shown to be associated with approximately one-quarter of all diarrhoeal admissions to hospital. Rotavirus infection predominantly occurs in infants less than 12 months of age (75%) and has a peak of shedding during the cooler, drier months of the year. A secondary peak during the spring has been observed. Multiple infections with rotavirus and at least one other microbial agent are common. The circulating VP7 serotypes and VP4 genotypes have been determined in various regions of South Africa and show a geographic specific distribution. A decade previously, P[8]G1 or G4 strains predominated, and P[4]G2 strains occurred in an epidemic pattern in one region. More recently, rotavirus strains with P[6] genotype have become common and novel VP7/VP4 genotype combinations are occurring across the country. G9 strains have been reported from Cape Town to Vendaland. The circulating rotavirus types observed in this study add to the knowledge of the natural history of rotavirus infection and provide the groundwork to consider future vaccine strategies.
Subject(s)
Rotavirus Infections/epidemiology , Rotavirus Infections/immunology , Rotavirus Vaccines , Child, Preschool , Diarrhea/epidemiology , Diarrhea/etiology , Genotype , Humans , Infant , Infant, Newborn , Rotavirus Infections/complications , Seasons , Serotyping , South Africa/epidemiologyABSTRACT
A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted NDV-infected allantoic fluid and found to be 10(-5) HA units. Application of one-step RT-PCR to various NDV samples, including wild-type virulent isolates and avirulent vaccine strains, demonstrated the potential for rapid identification (3-4 h) of NDV isolates as well as the differentiation of virulent from avirulent strains.
Subject(s)
Newcastle Disease/virology , Newcastle disease virus/isolation & purification , Animals , Birds , Chick Embryo , Newcastle disease virus/classification , Newcastle disease virus/genetics , Newcastle disease virus/pathogenicity , RNA, Viral/analysis , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Sensitivity and Specificity , Specific Pathogen-Free Organisms , VirulenceABSTRACT
OBJECTIVE: To determine the incidence of Rotavirus infection in children under five years of age presenting with diarrhoea. DESIGN: Cross sectional study. SETTING: Mapulaneng Hospital, Bushbuckridge, Republic of South Africa. SUBJECTS: 50 children under five years of age presenting with diarrhoea. MAIN OUTCOME MEASURES: Rotavirus seroprevalence. RESULTS: Out of 50, 24 (48%) had detectable Rotavirus particles in their stools. The highest incidence of infection was observed among the seven to 12 months age group. Out of the 245 positive subjects 22 (91.7%) were breast fed. CONCLUSION: This study shows that diarrhoea in children under five years of age is probably caused by Rotavirus in Bushbuckridge. We therefore recommend that all children presenting with diarrhoea should be screened for Rotavirus.
Subject(s)
Diarrhea/virology , Rotavirus Infections/epidemiology , Cross-Sectional Studies , Diarrhea/epidemiology , Feces/virology , Female , Humans , Incidence , Infant , Infant, Newborn , Male , Rotavirus/isolation & purification , South Africa/epidemiologyABSTRACT
PIP: The training division of the Los Angeles Regional Family Planning Council conducted a seminar for minority members to discover attitudes toward birth control. Indian-Americans, Mexican-Americans, Asian-Americans, and Black-Americans participated. Some fears and misunderstandings relative to birth control were voiced. The timidity of recent Chinese immigrants was mentioned. Male reluctance to use birth control out of a sense of machismo was mentioned in relation to Black- and Mexican-Americans. The conclusion was that, when birth control methods are adequately explained, women of all ethnic groups are willing to use them. To allay fears, suspicions, and concerns, family planning should be presented to minority communities as a health service and not a social service. Family planning clinics would be more accepted in these communities if they expanded their work into other health areas.^ieng