Species of the common discomycete genus Bisporella reassigned to at least four genera.

Bisporella as typically conceived is a genus of noticeable, bright yellow inoperculate discomycetes. This interpretation of the genus, however, is at odds with Bisporella pallescens, the current name of the type species of the genus; furthermore, the genus has been interpreted as including the unusual species Bisporella resinicola. By comparing morphological and molecular traits of species traditionally included in Bisporella, we show that the genus is polyphyletic, with many "typical" members of the genus belonging instead in Calycina in Pezizellaceae. Bisporella pallescens is conclusively linked with its asexual morph, Bispora antennata, and the genus Bisporella is abandoned as a later synonym of the monotypic genus Bispora (previously applied only to asexual fungi) and placed as sister to Hymenoscyphus in Helotiaceae. Bisporella resinicola is shown to represent an independent monotypic genus, Eustilbum, which so far is placed incertae sedis in Helotiales. Finally, "Bisporella" subpallida, like Bispora, belongs to Helotiaceae but is instead related to "Phaeohelotium" epiphyllum.

Connectomes across development reveal principles of brain maturation.

An animal's nervous system changes as its body grows from birth to adulthood and its behaviours mature1-8. The form and extent of circuit remodelling across the connectome is unknown3,9-15. Here we used serial-section electron microscopy to reconstruct the full brain of eight isogenic Caenorhabditis elegans individuals across postnatal stages to investigate how it changes with age. The overall geometry of the brain is preserved from birth to adulthood, but substantial changes in chemical synaptic connectivity emerge on this consistent scaffold. Comparing connectomes between individuals reveals substantial differences in connectivity that make each brain partly unique. Comparing connectomes across maturation reveals consistent wiring changes between different neurons. These changes alter the strength of existing connections and create new connections. Collective changes in the network alter information processing. During development, the central decision-making circuitry is maintained, whereas sensory and motor pathways substantially remodel. With age, the brain becomes progressively more feedforward and discernibly modular. Thus developmental connectomics reveals principles that underlie brain maturation.

Sareomycetes: more diverse than meets the eye.

Since its resurrection, the resinicolous discomycete genus Sarea has been accepted as containing two species, one with black apothecia and pycnidia, and one with orange. We investigate this hypothesis using three ribosomal (nuITS, nuLSU, mtSSU) regions from and morphological examination of 70 specimens collected primarily in Europe and North America. The results of our analyses support separation of the traditional Sarea difformis s.lat. and Sarea resinae s.lat. into two distinct genera, Sarea and Zythia. Sarea as circumscribed is shown to conservatively comprise three phylospecies, with one corresponding to Sarea difformis s.str. and two, morphologically indistinguishable, corresponding to the newly combined Sarea coeloplata. Zythia is provisionally maintained as monotypic, containing only a genetically and morphologically variable Z. resinae. The new genus Atrozythia is erected for the new species A. klamathica. Arthrographis lignicola is placed in this genus on molecular grounds, expanding the concept of Sareomycetes by inclusion of a previously unknown type of asexual morph. Dating analyses using additional marker regions indicate the emergence of the Sareomycetes was roughly concurrent with the diversification of the genus Pinus, suggesting that this group of fungi emerged to exploit the newly-available resinous ecological niche supplied by Pinus or another, extinct group of conifers. Our phylogeographic studies also permitted us to study the introductions of these fungi to areas where they are not native, including Antarctica, Cape Verde, and New Zealand and are consistent with historical hypotheses of introduction.

Enumeration of sublethally injured Escherichia coli O157:H7 ATCC 43895 and Escherichia coli strain B-41560 using selective agar overlays versus commercial methods.

Quality control procedures during food processing may involve direct inoculation of food samples onto appropriate selective media for subsequent enumeration. However, sublethally injured bacteria often fail to grow, enabling them to evade detection and intervention measures and ultimately threaten the health of consumers. This study compares traditional selective and nonselective agar-based overlays versus two commercial systems (Petrifilm and Easygel) for recovery of injured E. coli B-41560 and O157:H7 strains. Bacteria were propagated in tryptic soy broth (TSB), ground beef slurry, and infant milk formula to a density of 10(6) to 10(8) CFU/ml and then were stressed for 6 min either in lactic acid (pH 4.5) or heat shocked for 3 min at 60°C. Samples were pour plated in basal layers of either tryptic soy agar (TSA), sorbitol MacConkey agar (SMAC), or violet red bile agar (VRB) and were resuscitated for 4 h prior to addition of agar overlays. Other stressed bacteria were plated directly onto Petrifilm and Easygel. Results indicate that selective and nonselective agar overlays recovered significantly higher numbers (greater than 1 log) of acid- and heat-injured E. coli O157:H7 from TSB, ground beef, and infant milk formula compared with direct plating onto selective media, Petrifilm, or Easygel, while no significant differences among these media combinations were observed for stressed E. coli B-41560. Nonstressed bacteria from TSB and ground beef were also recovered at densities significantly higher in nonselective TSA-TSA and in VRB-VRB and SMAC-SMAC compared with Petrifilm and Easygel. These data underscore the need to implement food safety measures that address sublethally injured pathogens such as E. coli O157:H7 in order to avoid underestimation of true densities for target pathogens.

The influence of six pharmaceuticals on freshwater sediment microbial growth incubated at different temperatures and UV exposures.

Pharmaceutical compounds have been detected in freshwater for several decades. Once they enter the aquatic ecosystem, they may be transformed abiotically (i.e., photolysis) or biotically (i.e., microbial activity). To assess the influence of pharmaceuticals on microbial growth, basal salt media amended with seven pharmaceutical treatments (acetaminophen, caffeine, carbamazepine, cotinine, ibuprofen, sulfamethoxazole, and a no pharmaceutical control) were inoculated with stream sediment. The seven pharmaceutical treatments were then placed in five different culture environments that included both temperature treatments of 4, 25, 37°C and light treatments of continuous UV-A or UV-B exposure. Microbial growth in the basal salt media was quantified as absorbance (OD(550)) at 7, 14, 21, 31, and 48d following inoculation. Microbial growth was significantly influenced by pharmaceutical treatments (P < 0.01) and incubation treatments (P < 0.01). Colonial morphology of the microbial communities post-incubation identified selection of microbial and fungal species with exposure to caffeine, cotinine, and ibuprofen at 37°C; acetaminophen, caffeine, and cotinine at 25°C; and carbamazepine exposed to continuous UV-A. Bacillus and coccus cellular arrangements (1000X magnification) were consistently observed across incubation treatments for each pharmaceutical treatment although carbamazepine and ibuprofen exposures incubated at 25°C also selected spiral-shaped bacteria. These data indicate stream sediment microbial communities are influenced by pharmaceuticals though physiochemical characteristics of the environment may dictate microbial response.

Development of a submerged-liquid sporulation medium for the johnsongrass bioherbicide Gloeocercospora sorghi.

Submerged culture experiments were conducted in three phases to determine the optimal medium for rapidly producing conidia of the fungal bioherbicide Gloeocercospora sorghi. In phase I, 18 crude carbon sources were evaluated to determine which would support sporulation. Under the conditions tested, butter bean and lima bean brines (1.5-4.6 mS/cm) provided best conidiation. In phase II, a fractional-factorial design was utilized to screen 76 different medium adjuncts in combination with butter bean brine for improved sporulation. d-Mannitol and carboxymethylcellulose (CMC) were the only acceptable factors that resulted in a significant improvement. In phase III, a central composite design with response surface methodology was used to optimize concentrations of these critical factors. The model predicted optimal sporulation in a medium composed of 2.69 mS/cm butter bean brine +0.043 M d-mannitol +0.37% w/v CMC with an expected titer of 1.51x10(7) conidia/ml. Actual mean titer attained with the model-derived medium was 1.91x10(7) conidia/ml. Optimal sporulation occurred at 25.5 degrees C in this medium and conidia remained viable up to 2.71 days when stored at 12 degrees C. No significant difference was observed in virulence of conidia produced on agar vs washed conidia produced in the model-derived (liquid) medium.

European river floods in a changing world.

Whereas the verdict is undecided about the effects of global warming on Europe's flood risks, it is clear that Europeans are becoming more exposed and vulnerable to floods. Losses are increasing dramatically, mainly because of population and capital moving into harm's way and also because of human-driven transformations of hydrological systems, including river basins and floodplains.

Guide to remedial measures for liquefaction mitigation at existing highway bridge sites

This document deals with the methodologies that were developed to assess liquefaction risk at existing highway bridges, select appropriate ground and/or foundation improvement methods to reduce the risk of damage, design the improvements, and verify that these improvements had been achieved in the field. The report describes the methodologies developed, methodologies to assess liquefaction risks are presented in flowchart form, progressing from simple to more complex methods. A number of remediation methods are considered, including grouting compaction, vibro systems, surcharge and buttress fills, reinforcement and containment, vertical drains and underpinning. Relevant information is summarized from some numerical and laboratory (shaking table centrifuge tests) studies conducted by other researchers on the performance of liquefaction remediation

Liquefaction criteria based on energy content of seismograms

Because energy is a more fundamental property or measure of earthquake excitation than peak acceleration and magnitude, several investigators have suggested that the amount of energy generated at specific points in a soil layer un response to earthquake shaking may correlate better with the development of liquefaction than with cyclic stress ratio. Kayen (1993) reviewed the literature on this topic and investigated yhe use of accelerogram energy, expressed in terms of Arias intensity as a parameter for use in evaluating liquefaction resistance of soils. What procedures should be used, and how accurately can Arias intensity be estimated for application at field sites where strong motion records are not avaible? has sufficient case history data and experience been compiled and analyzed to provide adequate verification of the procedure? The workshop encourages continued research to answer these questions and further develop energy procedures for use in engineering practice. (AU)

Evaluating effectiveness of liquefaction remediation measures for bridges

To effectively retrofit a bridge foundation sited on liquefiable soil deposits, it is necessary to determine the location and lateral extent of the soil block which should be treated by ground improcement techniques. Adequacy of a given treatment plan depends on limiting deformations to some tolerable level. Because the stability and deformation issues involved in such cases can be complex, the most sophisticated analysis methods may lie beyond the engineering budget and expertise available for smaller jobs. The current study focuses on developing simplified guidelines complexity for a typical liquefiable site. By examining the results of these analyses, preliminary conclusions have been reached regarding suitable methods of analysis, and the optimal location for ground treatment in similar cases.(AU)

Ground motion characteristics and their relation to soil liquefaction at the wildlife liquefaction array, Imperial Valley, California

We use the earthquake shaking severity parameter Arias intensity, Ih, to assess the site specific characteristics of ground motion, and investigate the relation of those characteristics to soil liquefaction occurrence at the Wildlife liquefaction array, in response to the Elmore Ranch and Superstition Hills earthquakes of November 23 and 24, 1987.(AU)

Evaluation of SPT-, CPT-, and shear wave-based methods for liquefaction potential assessment using Loma Prieta data

This study describes liquefaction-related damages near the East Bay shoreline, and provides an assessment of methods for prediction of liquefaction resistance based on SPT, CPT, and shear wave velocity measurements for five sites: (1) the Port of Richmond;(2) San Francisco-Oakland Bay Bridge Toll Plaza; (3)Port of Oakland, 7th St. Marine Container Facility; (4)Bay Farm Island; and(5) Oakland International Airport.(AU)

Prospects for improved hurricane protection on oceanic islands : Hawaii after hurricane Iwa

Hurricane Iwa (32 November 1982) produced Hawaii's costliest natural disaster and revealed serious flaws in the area's hurricane preparedness response and mitigation programs. Twenty-eight months later, a follow-up study showed that impacted communities and facilities had been reconstructed with only selective and limited attention to mitigation future coastal storm hazards. Prospects for the reduction of hazard vulnerability on oceanic island through post-disaster mitigation measures are assessed in the light of Hawaii's experience (AU)