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1.
Article in English | MEDLINE | ID: mdl-38662872

ABSTRACT

Maize is an important crop for the Republic of Moldova and one of the crops most contaminated with mycotoxins. Maize grain obtained from plants cultivated on Moldavian cornfields in 2021 and 2022 were tested for mycotoxigenic risk using qPCR with primers to several fungal genome sequences engaged in mycotoxin synthesis and ELISA test to screen total aflatoxins, fumonisin B1, zearalenone, deoxynivalenol and T-2 toxin. Except for T-2 toxin, the mycotoxin concentrations were under the limits of detection and did not exceed maximum admissible levels for unprocessed grain. Concentrations of T-2 toxin in grain samples did not correlate significantly with the quantity of toxigenic F. sporotrichioides. All of the analysed grain samples were contaminated with at least one toxigenic fungus, and 20% of the samples were infected with seven different species of toxigenic fungi. Accumulation of fungi in maize kernels was affected significantly by the season, and generally a decrease was observed in fungal frequency and quantity under drought conditions. However, several toxigenic Aspergillus and Fusarium fungi that are able to produce aflatoxins and fumonisins under improper storage conditions were found in the kernels during the whole period of monitoring.


Subject(s)
Food Contamination , Fungi , Mycotoxins , Zea mays , Zea mays/chemistry , Zea mays/microbiology , Mycotoxins/analysis , Mycotoxins/chemistry , Food Contamination/analysis , Fungi/isolation & purification , Moldova
2.
J Cell Biochem ; 119(8): 6857-6868, 2018 08.
Article in English | MEDLINE | ID: mdl-29693271

ABSTRACT

Low temperatures, salinity, and drought cause significant crop losses. These conditions involve osmotic stress, triggering transcriptional remodeling, and consequently, the restitution of cellular homeostasis and growth recovery. Protein transcription factors regulate target genes, thereby mediating plant responses to stress. bZIP17 is a transcription factor involved in cellular responses to salinity and the unfolded protein response. Because salinity can also produce osmotic stress, the role of bZIP17 in response to osmotic stress was assessed. Mannitol treatments induced the transcript accumulation and protein processing of bZIP17. Transcriptomic analyses showed that several genes associated with seed storage and germination showed lower expression in bzip17 mutants than in wild-type plants. Interestingly, bZIP17 transcript was more abundant in seeds, and germination analyses revealed that wild-type plants germinated later than bzip17 mutants in the presence of mannitol, but no effects were observed when the seeds were exposed to ABA. Finally, the transcript levels of bZIP17 target genes that control seed storage and germination were assessed in seeds exposed to mannitol treatments, which showed lower expression levels in bzip17 mutants compared to the wild-type seeds. These results suggest that bZIP17 plays a role in osmotic stress, acting as a negative regulator of germination through the regulation of genes involved in seed storage and germination.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Basic-Leucine Zipper Transcription Factors/metabolism , Gene Expression Regulation, Plant/physiology , Germination/physiology , Osmotic Pressure/physiology , Seeds/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Basic-Leucine Zipper Transcription Factors/genetics , Seeds/genetics
3.
PLoS One ; 10(4): e0122936, 2015.
Article in English | MEDLINE | ID: mdl-25860807

ABSTRACT

The unfolded protein response (UPR) is a signaling pathway that is activated when the workload of the endoplasmic reticulum (ER) is surpassed. IRE1 is a sensor involved in triggering the UPR and plays a key role in the unconventional splicing of an mRNA leading to the formation of a transcription factor that up-regulates the transcription of genes that play a role in restoring the homeostasis in the ER. In plants, bZIP60 is the substrate for IRE1; however, questions such as what is the dynamics of the splicing of bZIP60 and the fate of the proteins encoded by the spliced and unspliced forms of the mRNA, remain unanswered. In the present work, we analyzed the processing of bZIP60 by determining the levels of the spliced form mRNA in plants exposed to different conditions that trigger UPR. The results show that induction of ER stress increases the content of the spliced form of bZIP60 (bZIP60s) reaching a maximum, that depending on the stimuli, varied between 30 min or 2 hrs. In most cases, this was followed by a decrease in the content. In contrast to other eukaryotes, the splicing never occurred to full extent. The content of bZIP60s changed among different organs upon induction of the UPR suggesting that splicing is regulated differentially throughout the plant. In addition, we analyzed the distribution of a GFP-tagged version of bZIP60 when UPR was activated. A good correlation between splicing of bZIP60 and localization of the protein in the nucleus was observed. No fluorescence was observed under basal conditions, but interestingly, the fluorescence was recovered and found to co-localize with an ER marker upon treatment with an inhibitor of the proteasome. Our results indicate that the dynamics of bZIP60, both the mRNA and the protein, are highly dynamic processes which are tissue-specific and stimulus-dependent.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/metabolism , Basic-Leucine Zipper Transcription Factors/genetics , RNA Splicing , RNA, Messenger/metabolism , Unfolded Protein Response , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis Proteins/metabolism , Basic-Leucine Zipper Transcription Factors/metabolism , Cell Nucleus/metabolism , Dithiothreitol/pharmacology , Electrophoresis, Capillary , Endoplasmic Reticulum/metabolism , Flowers/genetics , Flowers/metabolism , Mannitol/pharmacology , Microscopy, Confocal , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plants, Genetically Modified/metabolism , Proteasome Endopeptidase Complex/metabolism , RNA Splicing/drug effects , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Salicylic Acid/pharmacology , Sodium Chloride/pharmacology , Spectrometry, Fluorescence , Temperature , Transcriptional Activation , Tunicamycin/pharmacology , Unfolded Protein Response/drug effects
4.
J Cell Biochem ; 116(8): 1638-45, 2015 Aug.
Article in English | MEDLINE | ID: mdl-25704669

ABSTRACT

Plants can be severely affected by salt stress. Since these are sessile organisms, they have developed different cellular responses to cope with this problem. Recently, it has been described that bZIP17 and bZIP60, two ER-located transcription factors, are involved in the cellular response to salt stress. On the other hand, bZIP60 is also involved in the unfolded protein response (UPR), a signaling pathway that up-regulates the expression of ER-chaperones. Coincidentally, salt stress produces the up-regulation of BiP, one of the main chaperones located in this organelle. Then, it has been proposed that UPR is associated to salt stress. Here, by using insertional mutant plants on bZIP17 and bZIP60, we show that bZIP17 regulate the accumulation of the transcript for the chaperone BiP3 under salt stress conditions, but does not lead to the accumulation of UPR-responding genes such as the chaperones Calnexin, Calreticulin, and PDIL under salt treatments. In contrast, DTT, a known inducer of UPR, leads to the up-regulation of all these chaperones. On the other hand, we found that bZIP60 regulates the expression of some bZIP17 target genes under conditions were splicing of bZIP60 does not occur, suggesting that the spliced and unspliced forms of bZIP60 play different roles in the physiological response of the plant. Our results indicate that the ER-located transcription factors bZIP17 and bZIP60 play a role in salt stress but this response goes through a signaling pathway that is different to that triggered by the unfolded protein response.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/growth & development , Basic-Leucine Zipper Transcription Factors/genetics , Molecular Chaperones/genetics , Alternative Splicing , Arabidopsis/metabolism , Dithiothreitol/pharmacology , Gene Expression Regulation, Plant , Salinity , Stress, Physiological , Unfolded Protein Response/drug effects
5.
EMBO J ; 29(18): 3208-15, 2010 Sep 15.
Article in English | MEDLINE | ID: mdl-20711170

ABSTRACT

In Arabidopsis, the rapid-flowering summer-annual versus the vernalization-requiring winter-annual growth habit is determined by natural variation in FRIGIDA (FRI) and FLOWERING LOCUS C (FLC). However, the biochemical basis of how FRI confers a winter-annual habit remains elusive. Here, we show that FRI elevates FLC expression by enhancement of histone methyltransferase (HMT) activity. EARLY FLOWERING IN SHORT DAYS (EFS), which is essential for FRI function, is demonstrated to be a novel dual substrate (histone H3 lysine 4 (H3K4) and H3K36)-specific HMT. FRI is recruited into FLC chromatin through EFS and in turn enhances EFS activity and engages additional HMTs. At FLC, the HMT activity of EFS is balanced by the H3K4/H3K36- and H3K4-specific histone demethylase (HDM) activities of autonomous-pathway components, RELATIVE OF EARLY FLOWERING 6 and FLOWERING LOCUS D, respectively. Loss of HDM activity in summer annuals results in dominant HMT activity, leading to conversion to a winter-annual habit in the absence of FRI. Thus, our study provides a model of how growth habit is determined through the balance of the H3K4/H3K36-specific HMT and HDM activities.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/growth & development , Flowers/growth & development , Gene Expression Regulation, Plant , Histones/metabolism , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Chromatin/metabolism , Flowers/genetics , Flowers/metabolism , Histone Methyltransferases , Histone-Lysine N-Methyltransferase/metabolism , Methylation
6.
Plant J ; 43(1): 29-46, 2005 Jul.
Article in English | MEDLINE | ID: mdl-15960614

ABSTRACT

AUXIN RESPONSE FACTORS (ARFs) regulate auxin-mediated transcriptional activation/repression. They are encoded by a gene family in Arabidopsis, and each member is thought to play a central role in various auxin-mediated developmental processes. We have characterized three arf2 mutant alleles, arf2-6, arf2-7 and arf2-8. The mutants exhibit pleiotropic developmental phenotypes, including large, dark green rosette leaves, delayed flowering, thick and long inflorescence, abnormal flower morphology and sterility in early formed flowers, large organ size and delayed senescence and abscission, compared with wild-type plants. In addition, arf2 mutant seedlings have elongated hypocotyls with enlarged cotyledons under various light conditions. The transcription of ACS2, ACS6 and ACS8 genes is impaired in the developing siliques of arf2-6. The phenotypes of all three alleles are similar to those of the loss-of-function mutants obtained by RNA interference or co-suppression. There is no significant effect of the mutation on global auxin-regulated gene expression in young seedlings, suggesting that ARF2 does not participate in auxin signaling at that particular developmental stage of the plant life cycle. Because ARF2 is thought to function as a transcriptional repressor, the prospect arises that its pleiotropic effects may be mediated by negatively modulating the transcription of downstream genes in signaling pathways that are involved in cell growth and senescence.


Subject(s)
Arabidopsis Proteins/physiology , Arabidopsis/growth & development , Arabidopsis/genetics , Repressor Proteins/physiology , Chromosome Mapping , Chromosomes, Plant , Flowers/growth & development , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Microarray Analysis , Mutation , Phenotype , Plant Diseases , Plant Leaves/growth & development , Plant Stems/growth & development , Seeds
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