ABSTRACT
On days 1 and 4 after fractionated irradiation of rats with dose of 0.75 Gy changes in thymocyte and lymphocyte plasma membrane structures were studied. Structural parameters of fluorescence probe auramin binding, viscosity of free lipids and lipids bound with integral proteins as well as structural changes of membrane protein structure were determined. It was shown that after exposure to low doses the changes in membrane protein structures occurred both in thymocytes and lymphocytes. The changes in viscosity of membrane lipids and patterns of auramin binding were more pronounced in lymphocytes than in thymocytes. It is suggested that radiosensitivity of lymphocytes is higher than that of thymocytes.
Subject(s)
Lymphocytes/radiation effects , Thymus Gland/radiation effects , Animals , Benzophenoneidum , Cell Membrane/metabolism , Cell Membrane/radiation effects , Cell Membrane/ultrastructure , Dose-Response Relationship, Radiation , Fluorescent Dyes , Lymphocytes/metabolism , Lymphocytes/ultrastructure , Male , Membrane Lipids/radiation effects , Membrane Proteins/radiation effects , Rats , Rats, Wistar , Thymus Gland/metabolism , Thymus Gland/ultrastructure , Time Factors , Viscosity/radiation effectsSubject(s)
Bombesin/blood , Neoplasms/blood , Neurotensin/blood , Adult , Female , Humans , Male , Middle Aged , RadioimmunoassayABSTRACT
Some structural and functional indices of the genome state of rat myelopoietic cells after cytostatic ingestion are studied by the immunofluorescent method and by 3H-thymidine incorporation. It is shown that administration of cyclophosphamide causes structural and functional damages of hemopoietic cells. It is suggested that the intensity of repair potentials is a leading factor in restoration of depressed hemopoiesis.
Subject(s)
Blood Cells/drug effects , Bone Marrow/drug effects , Cyclophosphamide/pharmacology , DNA Damage , DNA/drug effects , Animals , Blood Cells/cytology , Blood Cells/metabolism , Bone Marrow/metabolism , Bone Marrow Cells , Cell Count/drug effects , DNA/biosynthesis , Depression, Chemical , Male , Rats , Rats, Inbred Strains , Research Design , gamma-Globulins/pharmacologyABSTRACT
Functional state of genetic apparatus was studied in leukocytes of patients with carcinoma of mammary gland at the III-IV stage by measuring incorporation of 3H-thymidine into DNA as well as by monitoring impairment of DNA structure using immunofluorescent procedure and antibodies to DNA. The studies were carried out before, in the middle period and at the end of the antitumour chemotherapy. The structure-functional state of genetic apparatus was injured in circulating leukocytes in blood of oncological patients.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/adverse effects , Breast Neoplasms/blood , DNA, Neoplasm/biosynthesis , Leukocytes/metabolism , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Breast Neoplasms/drug therapy , Breast Neoplasms/genetics , Female , Humans , Leukocytes/drug effects , Leukopenia/chemically induced , PrognosisABSTRACT
Impairments in DNA from brain stem cell nuclei of X-ray irradiated animals were studied at early steps after irradiation using antibodies to single-stranded DNA. A possibility was shown to detect by means of an immunochemical technique the irradiation-induced impairments of DNA in brain stem cells. Detection of the impairment was possible within 3 hrs after high doses of the irradiation (206.4-258 mCi/kg); after lower doses - within the first hour of the postirradiation period. The immunochemical procedure might be used for early biological detection of the irradiation impairments as well as for estimation of radio-sensitivity of nuclear cells to the effects of irradiation and radioprotectors.
Subject(s)
Antibodies/immunology , Bone Marrow/radiation effects , Cell Nucleus/radiation effects , DNA/immunology , Radiation Injuries, Experimental/diagnosis , Animals , Antibodies/analysis , Antibody Specificity , Bone Marrow/immunology , Cell Nucleus/immunology , DNA/radiation effects , DNA, Single-Stranded/immunology , Fluorescent Antibody Technique , Immunization , Male , Rabbits , Radiation Injuries, Experimental/immunology , Rats , Time FactorsABSTRACT
Distribution of a label was studied in nuclear fraction and in tissues of rats with transplanted sarcoma 45 within a day after an administration of immune and nonimmune 131I-gamma-globulins. When gamma-globulins, containing antibodies to one-stranded DNA, were administered the more intensive accumulation of the label was observed in nuclei of malignant tissue as compared with other tissues (liver, heart, thymus and muscles). The diffuse distribution of the label was found if nonimmune gamma-globulins were used. The phenomenon was apparently related to the active penetration of labelled antibodies to the replicating matrix and binding of them with one-stranded DNA sites. The suggestion is discussed that the effect of accumulation of immune gamma-globulin in proliferating tissue might be used for depression of the proliferative process.
