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Microbiologyopen ; 6(4)2017 08.
Article in English | MEDLINE | ID: mdl-28371280

ABSTRACT

The effect of elevated atmospheric CO2 concentration [CO2 ] on the diversity and composition of the prokaryotic community inhabiting the rhizosphere of winter barley (Hordeum vulgare L.) was investigated in a field experiment, using open-top chambers. Rhizosphere samples were collected at anthesis (flowering stage) from six chambers with ambient [CO2 ] (approximately 400 ppm) and six chambers with elevated [CO2 ] (700 ppm). The V4 region of the 16S rRNA gene was PCR-amplified from the extracted DNA and sequenced on an Illumina MiSeq instrument. Above-ground plant biomass was not affected by elevated [CO2 ] at anthesis, but plants exposed to elevated [CO2 ] had significantly higher grain yield. The composition of the rhizosphere prokaryotic communities was very similar under ambient and elevated [CO2 ]. The dominant taxa were Bacteroidetes, Actinobacteria, Alpha-, Gamma-, and Betaproteobacteria. Elevated [CO2 ] resulted in lower prokaryotic diversity in the rhizosphere, but did not cause a significant difference in community structure.


Subject(s)
Bacteria/classification , Bacteria/genetics , Biota/drug effects , Carbon Dioxide/metabolism , Soil Microbiology , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Hordeum/growth & development , Phylogeny , RNA, Ribosomal, 16S/genetics , Rhizosphere , Sequence Analysis, DNA
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