ABSTRACT
A triplex-forming oligonucleotide (TFO) can bind to genomic DNA and inhibit the expression of genes with specific sequences. However, to date, there have been a few reports of modified bases in antiparallel TFOs that can recognize and strongly bind to T-A base pairs. In this study, we introduced several quinoline derivatives into antiparallel TFOs to develop modified bases that can recognize the T-A base pair and evaluated their ability to form triplexes and to discriminate between base pairs. The introduction of 2-acetamido-6-aminoquinoline6DAQac) into an antiparallel TFO allowed the selective recognition of a T-A base pair at both low and high salt concentrations.
Subject(s)
DNA , Oligonucleotides , Base Pairing , DNA/metabolism , Molecular Structure , Nucleic Acid ConformationABSTRACT
For the advancement of nucleic acid-related research, high-efficiency, low-cost synthesis of high-purity oligonucleotides is necessary. Herein, we introduced hydroxybenzotriazole (HOBt) activators on controlled pore glass resins to improve the efficiency of chain elongation (the synthesis efficiency increased from 48% without an activator to 92% with an activator). In particular, the use of the resin containing 6-trifluoromethyl HOBt with a linker of lauric acid and succinic acid significantly improved the synthesis efficiency for both DNA and RNA syntheses.