ABSTRACT
In this quantitative, histological study, both goblet cell number and distribution of secretory granules in normal septal mucosa of rats were examined by computer-based color image analysis combined with a new, simple whole-mount method. Our new method has proved to be a very useful and highly accurate means of examination. The count and size of the mucous area of goblet cells decreased toward the antero-posterior direction with the exception of the apical region of the septum. The regional difference characteristic of the goblet cell is noteworthy. The results are assumed to be closely related to both flow direction of mucous blanket and distribution of nasal gland orifices. Goblet cells are assumed to be controlled by some regulatory factor of the secretory system and to play an important role in nasal physiological mechanism.
Subject(s)
Nasal Mucosa/cytology , Nasal Septum/cytology , Animals , Cell Count , Color , Cytoplasmic Granules , Image Processing, Computer-Assisted/methods , Male , Rats , Rats, WistarABSTRACT
Sixty-three ears of 49 patients with serous otitis media were treated making use of butterfly ventilation tube in our 4 hospitals and 1 private office. The average time from intubation to extubation was 9 months and the longest case was 33 months. Main complications were infection and spontaneous extubation. The rate of hearing improvement after tympanostomy was more than 80% in all cases. The frequency of the most improvement was observed in 1kHz on the average. It was cleared that the butterfly ventilation tube was easy to use for the wide age patients and at any clinics. It was concluded that the butterfly ventilation tube was useful as a long-term ventilation tube.
Subject(s)
Middle Ear Ventilation/methods , Otitis Media with Effusion/therapy , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Evaluation Studies as Topic , Hearing , Humans , Middle Aged , Middle Ear Ventilation/adverse effects , Otitis Media with Effusion/physiopathology , Time FactorsABSTRACT
The light microscopical and ultracytochemical localization of Na-K ATPase (ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase) in the guinea pig endolymphatic sac was studied by a newly developed lead citrate one step method. The cytochemical reaction product was detected in the epithelial cell layer of the endolymphatic sac at the light microscopical level. At the electron microscopical level, the reaction product was located to the basolateral plasma membrane of both the dark and the light cells. The results suggest that Na-K ATPase may play a role in the ion transport mechanism in the endolymphatic sac.
Subject(s)
Ear, Inner/enzymology , Endolymphatic Sac/enzymology , Sodium-Potassium-Exchanging ATPase/analysis , Animals , Endolymphatic Duct/enzymology , Female , Guinea Pigs , Ion Exchange , MaleABSTRACT
Triphosphoinositide (TPI), an aminoglycoside receptor and a possible regulator of cationic permeation through its ability to bind with Ca++, was localized by the protein-A gold technique in vestibular sensory epithelia using an antibody highly specific to TPI. TPI was detected on the stereocilia, kinocilia, and cuticular plate of hair cells, and in the reticular membrane of supporting cells. The cilia of hair cells are damaged by aminoglycosides at a relatively early stage of toxicity. Ca++-regulated bioactivity in this area is probably involved.
Subject(s)
Hair Cells, Auditory/analysis , Phosphatidylinositol Phosphates , Phosphatidylinositols/analysis , Aminoglycosides/toxicity , Animals , Gold , Guinea Pigs , Hair Cells, Auditory/drug effects , Histocytochemistry , Staphylococcal Protein AABSTRACT
Triphosphoinositide (TPI) has been demonstrated to be a receptor for aminoglycosides in the cochlea and may regulate ionic permeability by its binding with Ca++. This phospholipid was localized by a protein A-gold technique in the cochlea at the electronmicroscopic level. TPI was prepared by a neomycin column and antibodies to it were raised in rabbits. The antibody used in this study reacted virtually only to TPI among the tested lipids. TPI was localized mainly at stereocilia, cuticular plates, head plates of Deiters' cells, plasma membrane, and mitochondria of various cells in the organ of Corti. In the vascular stria, TPI was found mainly at the plasma membrane of basal infoldings of the marginal cells. Possible physiological and pathophysiological roles of TPI in the cochlea are briefly discussed.
Subject(s)
Cochlea/metabolism , Phosphatidylinositol Phosphates , Phosphatidylinositols/analysis , Animals , Cochlea/ultrastructure , Female , Gold , Guinea Pigs , Hair Cells, Auditory, Inner/ultrastructure , Histocytochemistry/methods , Immunochemistry/methods , Male , Microscopy, Electron , Staining and Labeling , Staphylococcal Protein AABSTRACT
The clinical effect of cefsulodin (CFS) on Pseudomonas species infections in otorhinolaryngologic field was studied in 10 patients. The overall clinical effectiveness was 80% (excellent 4, good 4, poor 2). The bacteriological response of CFS against Pseudomonas species was evaluated from 11 strains. Eradication rate of Pseudomonas species was 82%. In 1 case, body temperature was rised after injection. No abnormal laboratory findings, relating to the drug, were observed after treatment.
