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1.
Res Vet Sci ; 81(2): 280-6, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16545849

ABSTRACT

The angiopoietin (Ang) family of proteins are central to the regulation of angiogenesis. The purposes of this study were to determine cDNA sequences of canine Ang-1 and Ang-2 and investigate their expressions in normal tissues and spontaneous tumours. The cDNA sequences of canine Ang-1 and Ang-2 were 1,494 and 1,488 bp, and the deduced amino acid sequences were 497 and 495 residues, respectively. The cDNA sequences of canine Ang-1 and Ang-2 showed high homology with those of the other mammalian species. Canine Ang-1 and Ang-2 mRNA were detectable in all 22 normal tissues and spontaneous tumours. Higher mRNA expression level of canine Ang-2 was demonstrated in mammary simple carcinomas, haemangiosarcoma and hepatocellular carcinoma in comparison with normal tissues.


Subject(s)
Angiopoietin-1/biosynthesis , Angiopoietin-2/biosynthesis , Dog Diseases/genetics , Neoplasms/veterinary , Amino Acid Sequence , Angiopoietin-1/genetics , Angiopoietin-2/genetics , Animals , Base Sequence , Dog Diseases/metabolism , Dogs , Gene Expression , Molecular Sequence Data , Neoplasms/genetics , Neoplasms/metabolism , Neovascularization, Pathologic/genetics , Neovascularization, Pathologic/metabolism , Neovascularization, Pathologic/veterinary , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Sequence Alignment , Vascular Endothelial Growth Factor A/biosynthesis , Vascular Endothelial Growth Factor A/genetics
3.
Kango Kyoiku ; 23(7): 446-53, 1982 Jul.
Article in Japanese | MEDLINE | ID: mdl-6922244

Subject(s)
Nurses , Social Perception , Japan
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