Formulation and Applications of Lipid-Based Nanovehicles: Spotlight on Self-emulsifying Systems.

The drug delivery investigation field is continuously widened and adapted to overcome many factors such as poor drug solubility, absorption, rapid metabolism, the variability of drug plasma levels, cellular efflux and many others. Due to resemblance to body constituents and their biocompatibility, lipids offer a promising scheme for poorly water-soluble and lipophilic drugs. Various nanoparticles including vesicular systems, lipid particulate systems, and emulsion systems provide some unique benefits as pharmaceutical carriers in drug and biomolecules delivery systems. Nowadays synthesis is directed toward simple, costless techniques, therefore, self-emulsifying systems have gained superiority over the other carriers. Self nano-emulsifying systems composed of oil, surfactant, and co-surfactant emulsified upon contact with an aqueous medium, has been widely exploited. This review attempts to provide a comprehensive interpretation of different types of lipid-based carriers emphasizing on the self-nanoemulsifying system, why it is gaining interest, formulation, composition, and applications.

Tadalafil-Loaded Limonene-Based Orodispersible Tablets: Formulation, in vitro Characterization and in vivo Appraisal of Gastroprotective Activity.

BACKGROUND: Gastric ulcer is a prevalent disease with various etiologies, including non-steroidal anti-inflammatory drugs and alcohol consumption. This study aimed to explore the dual gastric protection effect of tadalafil and limonene as a self-nanoemulsifying system (SNES)-based orodispersible tablets. METHODS: Tadalafil-loaded limonene-based SNES was prepared, and the optimum formula was characterized in terms of particle size (PS), polydispersity index (PDI), and zeta potential (ZP) then loaded on various porous carriers to formulate lyophilized orodispersible tablets (ODTs). The ODTs were evaluated via determining hardness, friability, content uniformity, wetting, and disintegration time. The selected ODT was examined for its gastric ulcer protective effect against alcohol-induced ulcers in rat model. Ulcer score and ulcer index were computed for rats stomachs that were inspected macroscopically and histopathologically. RESULTS: The prepared SNES had droplet size of 104 nm, polydispersity index of 0.2, and zeta potential of -15.4 mV. From the different ODTs formulated, the formula with superior wetting time: 23.67 s, outstanding disintegration time: 28 s, accepted hardness value: 3.11 kg/cm2 and friability: 0.6% was designated. A significant gastroprotective effect of the unloaded and tadalafil-loaded ODTs was recognized compared to the omeprazole pre-treated group. Moreover, the histopathological analysis displayed very mild inflammation in the limonene-based ODTs group and intact structure in the tadalafil-loaded pre-treated animals. CONCLUSION: Limonene gastroprotective effect functioned along with tadalafil in the form of SNES-incorporated ODTs could serve as a promising revenue for better efficacy in gastric ulcer prevention.

Levofloxacin-loaded naturally occurring monoterpene-based nanoemulgel: a feasible efficient system to circumvent MRSA ocular infections.

Staphylococcus aureus is a leading cause of ocular keratitis worldwide, and the upsurge of Methicillin-resistant Staphylococcus Aureus (MRSA) strains necessitated the development of new antimicrobial agents. D-limonene is the major constituent of oil extracted from citrus peel, which has been utilized for its gastroprotective, antifungal, antitumor, and antibacterial effects. The present study aimed to develop an effective in-situ ocular limonene-based nanoemulgel to enhance the efficacy of fluoroquinolones against MRSA associated ocular biofilm infection. The nanoemulsion composed of limonene, Tween®80, propylene glycol at a ratio of 5:4:1 loaded with levofloxacin. The formulated levofloxacin-loaded limonene-based nanoemulsion physiochemical properties namely; droplet size, polydispersity index, zeta potential, and in-vitro drug release were studied and stability over three months was assessed. Furthermore, in-vitro antimicrobial susceptibility was investigated on biofilm-forming MRSA strain through kinetics of killing and biofilm assay. The in-situ nanoemulgel ocular irritation was studied by HET-CAM test. The results demonstrated that levofloxacin-loaded limonene-based nanoemulsion had a particle size of 119 ± 0.321 nm with improved eradicating efficacy of MRSA biofilm, where the MIC of the loaded nanoemulgel was 3.12 mg/ml significantly less than that of drug alone (6.25 mg/ml). HET-CAM test showed no signs of hemorrhage, coagulation, or lysis for the loaded nanoemulgel same as sodium chloride solution (negative control) where its irritation score was zero compared to 9.87 for the positive irritant group (1%w/v sodium lauryl sulfate). In conclusion, the current investigation provided a strong foundation for further studies of limonene nanoemulgel as a potential complementary therapeutic agent against resistant bacterial strains.

Updated but not outdated "Gliadin": A plant protein in advanced pharmaceutical nanotechnologies.

For many years, natural products have been considered as the backbone of medical therapy. Recently, different extracted active moieties of these botanical plants were employed as innovative drug delivery vehicles. Protein-based nanocarriers have acquired great engrossment as colloidal vehicles for delivering different therapeutic agents, anticancer, anti-inflammatory, vitamins, or even biomedical devices. Gliadin is a natural protein, accounts for 80-85% of the total wheat protein, and forms a vital part in the quality and nutritional value of flour. Gliadin is not just a dietary protein, its properties created a novel and surprising applications in medical fields, pharmaceutical, biomedical devices, and drug delivery. Gliadin is safe, biocompatible, and superior over synthetic proteins. The current review highlights the nature, characteristics, quantitation, and extraction of gliadin moreover sheds the light on its different applications. The high bioadhesive ability endorsed its interaction with gastric mucosa facilitating the delivery of various drug molecules, its structure, and the disulfide bonds expanded the encapsulation of lipophilic molecules, vitamins, and enzymes. Add on this its antimicrobial film capability and its flexibility as fibers. Gliadin is applied extensively in food and its safety to the patient with celiac disease, and cytotoxicity to normal cells has not been fully elaborated.