[The bacteriologic features of recurrent acute rhinosinusitis].

Objective:To investigate the bacteriologic characteristics of recurrent acute rhinosinusitis(RAR).Method:Twenty-nine patients (29 with RAR) from outpatient clinic in our hospital between June 2010 and May 2016 were enrolled in the study. Specimens of the middle meatus or olfactory cleft area using the sinus endoscopy through were transported to the laboratory for bacterial culture.Result:Twenty-five specimens out of 29 were bacterial culture positive (culture positive rate was 86.2%).A total of 32 isolates (25 aerobic or facultative and 7 anaerobic) were recovered from the 29 cases of RAR. The predominant aerobic or facultative bacteria were Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus. The predominant anaerobic bacteria were Bacteroides fragilis and Propionibacterium. Antibiotic susceptibility tests showed that the resistance rate of these aerobic or facultative bacteria to Macrolides (Erythromycin and Azithromycin) and quinolones (Levofloxacin) was 88% and 92%, respectively. Similarly, the resistance rate of bacteria to ß-lactamase antibiotics (penicillin, ampicillin, and cefazolin) was also greater than 90% (100%, 92%, and 92%, respectively). But the drug resistance of these bacteria to the thirdgeneration cephalosporin combined with beta lactamase inhibitors (Cefoperazone/sulbactam) was 20%. Among the 7 strains of anaerobic bacteria, 6 strains were sensitive to ornidazole.Conclusion:The major pathogens of RAR are the aerobic and facultative bacteria Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus, most of which are resistant to commonly used antibiotics.

TGF-ß1 and Serpine 1 expression changes in traumatic deep vein thrombosis.

The objective of this study was to investigate the expression changes of transforming growth factor ß1 (TGF-ß1) and Serpine 1 in rats with traumatic deep vein thrombosis (DVT). In total, 60 male Sprague Dawley rats were divided into model (N = 50) and control groups (Group A, N = 10). From the model group, 10 rats were randomly selected after modeling as the pre-thrombosis group (Group B, N = 10), and the remaining 40 rats in the model group were divided into the thrombosis (Group C) and no thrombosis groups (Group D) depending on whether DVT was apparent at 25 h after modeling. All rats were dissected and the total RNAs of the femoral veins were extracted. TGF-ß1 and Serpine 1 expression was detected by microarray and polymerase chain reaction (PCR) analyses, and the related signal pathways were analyzed using bioinformatic analysis. Of the 40 rats, DVT was evident in 23, yielding an incidence rate of 57.50%. TGF-ß1 and Serpine 1 expression increased significantly at 2.5 h after modeling, while DVT began to form at 25 h after modeling. Both PCR and microarray analysis showed that TGF-ß1 and Serpine 1 expression levels were significantly higher in the thrombosis group than in the other groups (P < 0.05). Bioinformatic analysis indicated that TGF-ß1 was an upstream regulatory gene of Serpine 1 and could induce Serpine 1 overexpression. Together, these results suggested that TGF-ß1 and Serpine 1 overexpression might play an important role in DVT formation and have predictive values.

Detection of targets and their mechanisms for early diagnosis of traumatic deep vein thrombosis.

The purpose of this investigation was to identify targets for the early diagnosis and predictors of deep venous thrombosis (DVT) and the role of these targets in the formation of venous thrombosis. A model of DVT was constructed in rats. Thromboses and venous walls were sampled for reverse transcription polymerase chain reaction study, and blood was sampled for enzyme-linked immunosorbent assay studies. Vein endothelial cells were cultured to observe the effects of interleukin (IL)-17 on the expression of tissue plasminogen activator (t-PA)/plasminogen activator inhibitor type 1 (PAI-1). IL-17 monoclonal antibody was used to study its effect on preventing the formation of DVT. One-hundred and twenty hours after the animal model was constructed, significant DVT started to form. Polymerase chain reaction tests showed that immediately after the model was created, the expression of IL-17 increased greatly, whereas the balance between t-PA and PAI-1 was disrupted just before DVT formed. The increase of serum IL-17 was positively related with the formation of DVT. Thus, the application of IL-17 monoclonal antibody could reduce the formation of DVT in rats. IL-17 might be a target for the early diagnosis of DVT and should be further studied to assess its clinical value.

The expression of non-clustered protocadherins in adult rat hippocampal formation and the connecting brain regions.

Non-clustered protocadherins (PCDHs) are calcium-dependent adhesion molecules which have attracted attention for their possible roles in the neuronal circuit formation during development and their implications in the neurological disorders such as autism and mental retardation. Previously, we found that a subset of the non-clustered PCDHs exhibited circuit-dependent expression patterns in thalamo-cortical connections in early postnatal rat brain, but such patterns disappeared in adulthood. In this study, we identified that the non-clustered PCDHs showed differential expression patterns along the septotemporal axis in the subregions of adult hippocampus and dentate gyrus with topographical preferences. The expressions of PCDH1, PCDH9, PCDH10 and PCDH20 showed septal preferences, whereas the expressions of PCDH8, PCDH11, PCDH17 and PCDH19 showed temporal preferences, suggesting that they play roles in the formation/maintenance of intrahippocampal circuits. PCDHs also exhibited the region-specific expression patterns in the areas connected to hippocampal formation such as entorhinal cortex, lateral septum, and basolateral amygdaloid complex. Furthermore, the expression levels of three PCDHs (PCDH8, PCDH19 and PCDH20) were regulated by the electroconvulsive shock stimulation of the brain in the adult hippocampus and dentate gyrus. These results suggest that non-clustered PCDHs are involved in the maintenance and plasticity of adult hippocampal circuitry.

Simultaneous measurement of dopamine and ascorbate at their physiological levels using voltammetric microprobe based on overoxidized poly(1,2-phenylenediamine)-coated carbon fiber.

Overoxidized poly-(1,2-phenylenediamine) (OPPD)-coated carbon fiber microelectrodes (CFMEs) exhibit, in combination with square-wave voltammetry (SWV) detection mode, the attractive ability to simultaneously measure low nM dopamine (DA) and mM ascorbate (AA) in a pH 7.4 medium. The PPD polymer film is electrodeposited onto a carbon fiber at a constant potential of 0.8 V versus Ag/AgCl using a solution containing sodium dodecylsulfate as the dopant. After overoxidation using cyclic voltammetry (CV) in the potential range from 0 to 2.2 V at a scan rate of 10 V/s, the resulting OPPD-CFME displays a high SWV current response to cationic DA at approximately 0.2 V and has a favorably low response to anionic AA at approximately 0.0 V vs Ag/AgCl. The preparation of the new OPPD-sensing film has been carefully studied and optimized. The OPPD properties and behavior were characterized using CV and SWV under various conditions and are discussed with respect to DA and AA detection. The linear calibration range for DA in the presence of 0.3 mM AA is 50 nM to 10 microM, with a correlation coefficient of 0.998 and a detection limit of 10 nM using 45-s accumulation. The detection limit for DA in the absence of AA was estimated to be 2 nM (S/N = 3). The linear range for AA in the presence of 100 nM DA is 0.2-2 mM, with a correlation coefficient of 0.999 and a detection limit of 80 microM. The reproducibilities of SWV measurements at OPPD-CFCMEs are 1.6% and 2.5% for 100 nM DA and 0.3 mM AA, respectively. Potential interfering agents, such as 3,4-dihydroxyphenylacetic acid, uric acid, oxalate, human serum proteins, and glucose, at their physiologically relevant or higher concentrations did not have any effect. These favorable features offer great promise for in vitro and in vivo application of the proposed OPPD-coated microprobe.