ABSTRACT
OBJECTIVE: To evaluate the clinical value of senescence marker protein 30 (SMP30) and anti-SMP30 antibody in serum. METHODS: We used enzyme-linked immunosorbent assay (ELISA) to analytically validate serum levels of SMP30 and anti-SMP30 antibody in 143 patients with hepatocellular carcinoma (HCC), compared with those levels in serum from 137 patients with chronic hepatitis (CH), 51 with liver cirrhosis (LC), and 165 healthy control individuals. RESULTS: The positivity rate of SMP30 in the HCC group (8.39%) was significantly higher than that rate in the CH group (.73%) and in the healthy control group (1.21%). The positivity rate for anti-SMP30 antibody in patients with HCC was 25.87%, that in the CH group was 4.38%, and that in the LC group was 3.92%. CONCLUSION: Anti-SMP30 antibody levels can be used as a biomarker for diagnosing HCC; marked results have been observed for patients with alpha-fetoprotein (AFP) negativity, in particular.
Subject(s)
Autoantibodies/blood , Calcium-Binding Proteins/blood , Carcinoma, Hepatocellular/diagnosis , Intracellular Signaling Peptides and Proteins/blood , Liver Neoplasms/diagnosis , Adolescent , Adult , Autoantibodies/immunology , Calcium-Binding Proteins/immunology , Carcinoma, Hepatocellular/blood , Carcinoma, Hepatocellular/epidemiology , Carcinoma, Hepatocellular/immunology , Female , Humans , Intracellular Signaling Peptides and Proteins/immunology , Liver Neoplasms/blood , Liver Neoplasms/epidemiology , Liver Neoplasms/immunology , Male , Middle Aged , Predictive Value of Tests , ROC Curve , Young AdultABSTRACT
Brown ducks carrying DHBV were widely used as hepatitis B animal model in the research of the activity and toxicity of anti-HBV dugs. Studies showed that the ratio of DHBV carriers in the brown ducks in Guilin region was relatively high. Nevertheless, the characters of the DHBV genome of Guilin brown duck remain unknown. Here we report the cloning of the genome of Guilin brown duck DHBV and the sequence analysis of the genome. The full length of the DHBV genome of Guilin brown duck was 3 027bp. Analysis using ORF finder found that there was an ORF for an unknown peptide other than S-ORF, PORF and C-ORF in the genome of the DHBV. Vector NTI 8. 0 analysis revealed that the unknown peptide contained a motif which binded to HLA * 0201. Aligning with the DHBV sequences from different countries and regions indicated that there were no obvious differences of regional distribution among the sequences. A fluorescence quantitative PCR for detecting DHBV was establishment based on the recombinant plasmid pGEM-DHBV-S constructed. This study laid the groundwork for using Guilin brown duck as a hepatitis B animal model.
