ABSTRACT
As people pay more attention to physical fitness and health keeping, running becomes the first choice for daily sport. However, due to the lack of scientific management and guidance, unreasonable running sometimes has a negative impact on health. The foot strike pattern has a great impact on the knee joint of the runner during running. Therefore, it is important for runners to monitor and record their running gait, so as to customize more appropriate training programs. Through cross-correlation analysis on two axial signals of the acceleration sensor, two common running landing gait patterns, the forefoot strike pattern and the rearfoot strike pattern, can be identified and distinguished. Based on the theoretical analysis, two running gait pattern recognition experiments were designed and conducted. Experiment results reveal that the method proposed can effectively characterize the two running gait patterns and shows a good universality and generalization ability among different subjects.
Subject(s)
Biosensing Techniques/instrumentation , Gait , Pattern Recognition, Automated , Running/physiology , Acceleration , Adult , Algorithms , Biomechanical Phenomena , Equipment Design , Female , Foot , Humans , Male , Models, Statistical , Shoes , Signal Processing, Computer-Assisted , Stress, Mechanical , Wearable Electronic Devices , Wireless Technology , Young AdultABSTRACT
OBJECTIVE: This study aims to investigate the regulatory mechanism of 1,25-(OH)2D3 on the proliferation of fibroblast-like synoviocytes (FLS) and expressions of pro-inflammatory cytokines in rheumatoid arthritis (RA) rats via microRNA-22 (miR-22). METHODS: A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments. Cell proliferation was detected by MTT. Cell cycle and apoptosis were analyzed by FCM. The IL-1ß, IL-6, IL-8, and PGE2 protein expressions were determined by ELISA, and MMP-3, INOS, and Cox-2 mRNA expressions were measured by qRT-PCR. RESULTS: The rat model of RA was successfully established. Compared with the blank group, the 1,25-(OH)2D3 and miR-22 inhibitors groups exhibited higher proliferation inhibition and apoptosis rates, lower levels of pro-inflammatory cytokines (IL-1ß, IL-6, IL-8, and PGE2), and decreased mRNA expressions of MMP-3, INOS, and Cox-2. The miR-22 mimics group had lower proliferation inhibition and apoptosis rates, elevated expressions of pro-inflammatory cytokines and MMP-3, INOS, and Cox-2 than the blank group. In contrast to the 1,25-(OH)2D3 group, the proliferation inhibition and apoptosis rates were down-regulated, and the expressions of pro-inflammatory cytokines and MMP-3, INOS, and Cox-2 were up-regulated in the 1,25-(OH)2D3 + miR-22 mimics group. CONCLUSION: Our study demonstrated that 1,25-(OH)2D3 inhibits the proliferation of FLS and alleviates inflammatory response in RA rats by down-regulating miR-22.
Subject(s)
Arthritis, Rheumatoid/pathology , Cholecalciferol/pharmacology , Cytokines/analysis , MicroRNAs/metabolism , Animals , Antagomirs/metabolism , Apoptosis , Arthritis, Rheumatoid/genetics , Arthritis, Rheumatoid/metabolism , Cell Proliferation , Cells, Cultured , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Cytokines/metabolism , Dinoprostone/metabolism , Disease Models, Animal , Down-Regulation , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Male , Matrix Metalloproteinase 3/genetics , Matrix Metalloproteinase 3/metabolism , MicroRNAs/antagonists & inhibitors , MicroRNAs/genetics , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , Rats , Rats, Sprague-Dawley , Synoviocytes/cytology , Synoviocytes/pathologyABSTRACT
The number of the apoplectic people is increasing while population aging is quickening its own pace. The precise measurement of walking speed is very important to the rehabilitation guidance of the apoplectic people. The precision of traditional measuring methods on speed such as stopwatch is relatively low, and high precision measurement instruments because of the high cost cannot be used widely. What's more, these methods have difficulty in measuring the walking speed of the apoplectic people accurately. UHF RFID tag has the advantages of small volume, low price, long reading distance etc, and as a wearable sensor, it is suitable to measure walking speed accurately for the apoplectic people. In order to measure the human walking speed, this paper uses four reader antennas with a certain distance to reads the signal strength of RFID tag. Because RFID tag has different RSSI (Received Signal Strength Indicator) in different distances away from the reader, researches on the changes of RSSI with time have been done by this paper to calculate walking speed. The verification results show that the precise measurement of walking speed can be realized by signal processing method with Gaussian Fitting-Kalman Filter. Depending on the variance of walking speed, doctors can predict the rehabilitation training result of the apoplectic people and give the appropriate rehabilitation guidance.
