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1.
J Med Microbiol ; 39(4): 273-81, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8411088

ABSTRACT

Antimicrobial susceptibility profiles of clinical and environmental isolates of Edwardsiella demonstrate that the three species are susceptible to beta-lactam antibiotics. All strains were susceptible to two quinolones tested and to gentamicin and doxycycline. E. tarda and E. hoshinae were resistant to clindamycin, whereas E. ictaluri was moderately susceptible. beta-Lactamase was produced by all strains of E. tarda, but not by E. hoshinae or E. ictaluri. A 54-kb plasmid was detected in six of 13 E. hoshinae strains. Five of the 10 E. tarda isolates studied gave an identical plasmid pattern of four plasmids ranging in size from 76-kb to 5.0-kb. One strain exhibited a 54-kb plasmid; four strains did not contain plasmid DNA. All E. ictaluri isolates contained a 5.7-kb and a 4.9-kb plasmid. E. tarda and E. ictaluri strains were resistant to human serum 20%; 12 of 13 strains of E. hoshinae were also serum resistant. Serum resistance may play an important part in the pathogenicity of these species.


Subject(s)
Anti-Bacterial Agents/pharmacology , Blood Bactericidal Activity , Enterobacteriaceae/physiology , Plasmids , beta-Lactamases/biosynthesis , Enterobacteriaceae/drug effects , Enterobacteriaceae/genetics , Enterobacteriaceae/pathogenicity , Humans , Microbial Sensitivity Tests , Species Specificity , Virulence
2.
Adv Perit Dial ; 9: 299-302, 1993.
Article in English | MEDLINE | ID: mdl-8105948

ABSTRACT

The average free choline level was determined to be 14 mumol/L in peritoneal dialysates and 22 mumol/L in the plasma of 30 patients on continuous ambulatory peritoneal dialysis (CAPD). Daily choline loss via dialysate averaged 129 mumol with 32 mumol choline lost per dwell. Daily choline loss via the dialysate was positively correlated with plasma choline concentrations. Choline levels in dialysate during CAPD exceed plasma levels of choline 9 mumol/L in healthy individuals.


Subject(s)
Choline/analysis , Dialysis Solutions/chemistry , Peritoneal Dialysis, Continuous Ambulatory , Choline/blood , Humans
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