Ubiquitin in combination with alcohol stimulates proliferative activity of hepatocytes.

There are several factors involved in aetiology of Alcoholic Liver Disease. The one of the most important role in regulation of intracellular transformations belongs to ubiquitin proteasome system that is comparably well studied, but less is known about the functions of extracellular ubiquitin. The goal of presented work is to study the effect of extracellular ubiquitin on partially hepatectomized alcoholic liver regeneration. Experiments were carried out on white female rats. Proliferative activity of hepatocytes was determined by colchicine mitotic index and immunohistochemical staining on Ki67. We have shown that in vivo injected extracellular ubiquitin stimulates proliferative activity of hepatocytes in partially hepatectomized alcoholic liver whereas in non-alcoholic rats hepatocytes proliferative activity was decreased. The main attraction of the experiment is the combination of alcohol and ubiquitin, as without alcohol ubiquitin inhibits proliferative activity of hepatocytes and blood cells. Further investigation of its action will provide new insights into possible pathway of ubiquitilation involved in stimulation of proliferative activity in alcoholic liver.

Pancreatic cell proteome - qualitative characterization and function.

The macromolecular protein complex form adult white rat pancreas has been obtained and partially characterized. The most important distinguishing characteristics of the complex is the thermostability of its components. Using polyacrylamid gel by electrophoresis and chromatography of hydrophobic interaction has been established, that the components with relatively high (45-60 kD) molecular weight, are hydrophobic, while the low molecular weight components (11-12 kD) corresponds to hydrophilic proteins according to its column retention time. Participation of TPC in the regulation of the homotypic cell growth was determined. It is shown that the pancreatic TPC through inhibition of transcription decreases cell mitotic activity in growing rats. It is also shown that this complex is involved in pancreas regeneration processes. On the basis of the qualitative characteristics and cell growth regulatory function of this macromolecular protein complex described by us, it can be considered as a pancreatic cell proteome.

In vivo investigation of extracellular ubiquitin effect on liver histoarchitectonics.

Ubiquitin is a small regulatory protein of living cells. It affects almost all cellular processes. Investigation of ubiquitin is very important for discovering the new therapeutics for different heavy human diseases. The main goal of presented work was to reveal effect of extracellular ubiquitin on liver histoarchitectonic of mature and immature white rats. The obtained data indicate the inefficiency of used doses--200 µg/ml and 500 µg/ml--of extracellular ubiquitin single and multiple in vivo injections to evident changes of histoarchitectonics of immature and mature white rat liver. Further investigation of in vivo injection effects on histoarchitectonics of intact and damaged liver and hepatocyte cell cycle regulation by immunological assays seems to be actual and interesting.

[Kinetic features of a white rat hepatocyte population under hormonal disbalance before and after partial hepatectomy].

Bilateral adrenalectomy, followed in 4 days by a partial hepatectomy, was performed using white rats weighing as much as 120-140 g. Under hormonal disbalance caused by bilateral adrenalectomy, the number of polyploid (4c, 4c x 2, and 8c) hepatocytes significantly increased, compared to that in non-operated control rats. Six hours after a partial hepatectomy, the share of highly ploid hepatocytes falls, being accompanied by a 9-fold increase in mitotic index. It is supposed that under hormonal disbalance condition, a partial hepatectomy may induce "early" mitoses in hepatocytes blocked in G2-phase of the cell cycle.