Subject(s)
Molluscum Contagiosum/complications , Mycosis Fungoides/complications , Skin Neoplasms/complications , Combined Modality Therapy , Humans , Interferon alpha-2 , Interferon-alpha/administration & dosage , Male , Middle Aged , Molluscum Contagiosum/immunology , Molluscum Contagiosum/therapy , Mycosis Fungoides/immunology , Mycosis Fungoides/therapy , PUVA Therapy , Recombinant Proteins , Skin Neoplasms/immunology , Skin Neoplasms/therapy , Th2 Cells/immunologyABSTRACT
A frozen section adhesion assay was used to study the influence of dapsone and colchicine on cell-cell adhesion between neutrophils and epidermal cells. Sections were prepared from small punch biopsies from healthy donors after preincubation with IFN-A. Freshly prepared neutrophils were activated with TNF-. or PAF after exposure to dapsone or colchicine, incubated on these sections, and the adhered cells were enumerated after washing. In the presence of dapsone (at concentrations of 0.1-80 Ig/ml) adherence of neutrophils could be blocked dose-dependently and 50% inhibition of adhesion was achieved at a concentration of 10 Ig/ml. Colchicine at concentrations of 10-200 ng/ml inhibited neutrophil binding with a clear correlation between inhibition and colchicine concentration. At a colchicine concentration of 110 ng/ml, 50% blocking was achieved. Since the function of CD11b/ paragraph signCD18 is necessary for neutrophil adhesion in this assay, we monitored CD11b expression on neutrophils after dapsone and colchicine exposure. CD11b expression on neutrophils was significantly reduced 5 h after incubation with dapsone at a concentration of 80 Ig/ paragraph signml, whereas no effects of colchicine on CD11b expression could be demonstrated. Our study showed that both drugs significantly inhibited adhesion between neutrophils and the epidermis.