Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease.

Representatives of Bacillus amyloliquefaciens were shown to possess biocontrol activity against fire blight, a serious disease of orchard trees caused by Erwinia amylovora. Genome analysis of B. amyloliquefaciens FZB42 identified gene clusters responsible for synthesis of several polyketide compounds with antibacterial action. We show here that the antibacterial polyketides difficidin and to a minor extent bacillaene act efficiently against E. amylovora. Surprisingly, a mutant strain blocked in the production of difficidin (CH8 Deltadfn) inhibited growth of E. amylovora and suppressed fire blight disease nearly in the same range as the wild type. In addition, a sfp mutant (CH3 Deltasfp) unable to synthesize non-ribosomally lipopeptides and polyketides did still suppress growth of E. amylovora, suggesting that besides action of polyketides another antagonistic principle exist. A double mutant (RS06 Deltasfp Deltabac) devoid in polyketide and bacilysin synthesis was unable to suppress growth of E. amylovora indicating that the additional inhibitory effect is due to production of bacilysin, a dipeptide whose synthesis does not depend on Sfp. We propose to use B. amyloliquefaciens strains with enhanced synthesis of difficidin and/or bacilysin for development of biocontrol agents efficient against fire blight disease.

Trends in PACS image storage and archive.

PACS is widely used in hospitals and is considered a mission critical system for around-the-clock daily clinical operation. Scheduled or unscheduled downtime of the main PACS archive storage or server could potentially cripple the entire PACS operation. This is especially the case in a filmless hospital environment. Therefore, in a downtime event, it is most desirable for users to have only a minimal performance impact without interruption of clinical data flow or loss of data and to have available historical PACS studies. This paper summarizes some of the developments in the design and implementation of a reliable PACS that insures maximum uptime for end users while preserving the integrity of the PACS data and making it available during downtime events. It also details strategy for developing proper clinical workflow contingency procedures when a scheduled downtime event to the main archive storage and server occurs. Specifically, the design and implementation of a fault-tolerant (FT) main archive server, the development of a FT back-up archive using an application service provider (ASP) model, and the clinical experiences while upgrading a main archive server and migrating the stored PACS data to new storage media will be discussed.

The effect of 10 : 1 compression and soft copy interpretation on the chest radiographs of premature neonates with reference to their possible application in teleradiology.

The aim of the study was to assess the potential application of teleradiology in the neonatal intensive care unit (NICU) by ascertaining whether any decrease in conspicuity of anatomic detail or interventional devices in the chest radiographs of premature infants is caused by picture archiving and communication system (PACS)-based soft copy interpretation of 10 : 1 compressed images. One hundred digital chest radiographs of low-birthweight infants were obtained in the NICU using a storage phosphor system. Laser-printed images were interpreted and the data set for each radiograph was then irreversibly compressed by a 10 : 1 ratio. Four radiologists with extensive PACS experience used a five-point grading system to score laser-printed hard copy images for the visibility of six parameters of anatomic landmarks and interventional devices in the chest. Compressed soft copy images displayed on 2K PACS workstation were subsequently scored using the same approach. Statistical manipulation demonstrated no loss of anatomic detail in five of the six parameters scored, with minimal difference in one landmark, the retrocardiac lung assessment. While further study is required to assess the clinical impact of the variance noted when evaluating lung parameters, the preservation or improvement of information in the remaining parameters following irreversible compression and soft copy interpretation is promising for the potential use of teleradiology in this population.

Three-dimensional ultrasonographic telepresence.

We have developed a three-dimensional ultrasound telepresence system for remote consultation. Three-dimensional ultrasound data-sets can be acquired by relatively unskilled operators. The data are stored in the remote unit and then transmitted to a consultant equipped with a similar unit. A telepresence pointing device enables the consultant to re-slice that data-set in any plane. During the study period, 72 volumetric scans of male and female volunteers aged 18-45 years were performed in Bosnia. Field users of limited ultrasound experience (most with less than 30 min of training) were able to acquire volumetric scans, send volume data and interact with remote consultants over standard communications lines at distances of up to 20,000 km. Communications links from 9.6 to 1500 kbit/s were used. Technical limitations included lack of motion data, lack of colour data, scan artefacts and increased scan-to-diagnosis time. However, our preliminary experience indicates that this technique may eventually prove to be a useful adjunct to telesonography. Further studies of the technique are needed to determine its value in the broader clinical setting.

Removal of obstructed Foley catheter from the urethra.

This case illustrates an approach to resolve a frustrating problem associated with Foley catheter balloon obstructions. By identifying its position in the urethra, the balloon can be deflated with minimal effort and limited urethra injury, using the suggested technique of transperineal needle insertion.