The effects of silver nanoparticles (Ag-NPs) sublethal concentrations on common carp (Cyprinus carpio): Bioaccumulation, hematology, serum biochemistry and immunology, antioxidant enzymes, and skin mucosal responses.

The present study aimed to evaluate the effects of different waterborne sublethal concentrations of Ag-NPs LC50 (96h) on common carp Cyprinus carpio using a multi-biomarker approach. Fish (9.22 ± 0.12 g) were stocked in fiberglass tanks and exposed to concentrations of 0 (control), 12.5%, 25% and 50% of Ag-NPs LC50 (96h) or Ag-NO3 LC50 (96h), as the source of Ag+ ion, for a period of 21 days. At the end of study, tissue Ag contents were significantly (P < 0.05) higher and different in fish exposed to concentrations of 25% and 50% compared to the control. The numbers of RBCs, hematocrit, and MCHC values at these concentrations differed significantly in respect to the control. No significant effects were observed for hemoglobin, MCH, and MCV values. The number of WBCs was significantly higher at concentrations of 12.5% and 25% compared to the control. Meanwhile, the percentage of neutrophils significantly elevated at concentrations of 25% and 50%. Serum total protein at concentration of 50% detected significantly lower than that of 12.5% or the control. The serum albumin and globulin levels significantly declined in Ag-NPs-exposed groups versus the control. The serum ACH50 and total immunoglobulins showed significantly lower values in the treatments of 25% and 50% compared to the control. The serum glucose, cortisol, ALT, and ALP values significantly escalated upon Ag-NPs exposure. The serum SOD and CAT showed enhanced activity in the treatment of 12.5% vice versa significantly diminished at concentrations of 25% and 50% compared to the control. The exposure to the concentrations of 25% and 50% significantly dwindled the lysozyme activity and total immunoglobulin levels in skin mucus. In conclusion, sublethal concentrations of Ag-NPs LC50 (96h) impaired fish health status at higher concentrations and 12.5% of Ag-NPs LC50 (96h) was presumably safe for common carp aquaculture.

Parkinsonism management issues in a patient with exhausted subthalamic deep brain stimulation battery, stricken by severe acute respiratory syndrome coronavirus 2.

Background: Nowadays, many neurological conditions, including Parkinson's disease (PD), are treated with deep brain stimulation (DBS). Life-threatening consequences can occur from DBS hardware failure or sudden implantable pulse generator (IPG) battery depletion. This issue may potentially worsen in concomitance with medical or infectious conditions, requiring stronger emergency management. Methods: We present here a 58 year-old PD patient with DBS, whose IPG replacement surgery was complicated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and we report management of this patient along with recommendations for patients with similar situation. Results: As the newly-emerged coronavirus disease 2019 (COVID-19) is now announced to be pandemic, new protocols and specific measures for each individual group of patients with chronic diseases seem obligatory. Regarding our recent experience with a patient suffering from PD, on DBS treatment, who needed hospitalization, we felt useful to share our experience as a recommended protocol for similar patients in the time of current pandemic. Conclusion: Close monitoring of laboratory and clinical signs should be warranted in patients with PD awaiting IPG replacement in order to be prepared in these novel conditions that may precipitate an akinetic crisis/dystonic storm and to prevent life-threatening complications during the current pandemic.

Prospective evaluation of a new "paper urease test" for ultra-rapid detection of Helicobacter pylori.

BACKGROUND: The purpose of this study was to determine the sensitivity, specificity, and positive and negative predictive values of a newly developed paper urease test (PUT) for ultra-rapid detection of helicobacter pylori (HP) in gastric mucosa. MATERIAL/METHODS: 100 patients (43 men) with a mean age of 57+/-6.8 years participated. Patients presenting for upper endoscopy with no recent exposure to HP-altering drugs were enrolled. Gastric biopsy specimens were tested by the PUT and histology methods, and then the patients underwent [13C] urea breath tests (UBT). HP was considered positive when either UBT or histology demonstrated it, and negative if HP was not detected in either UBT or histology. The PUT was reported at 15 minutes. RESULTS: 87 of 100 patients tested positive for HP. The PUT correctly identified 74 of 87 HP-positive and 13 of 13 HP-negative patients, yielding sensitivity, specificity, and positive and negative predictive values of 87%, 100%, 100% and 53.5%, respectively, in this population. CONCLUSIONS: Rapidly available and reliable results from the PUT can facilitate clinical decision prior to patient discharge from the endoscopy suite. We recommend PUT for screening of HP in endoscopy candidates, due to high specificity, rapid reaction, simplicity and low cost. A positive result shows a definite diagnosis, although a negative result needs further diagnostic methods.