Surveillance of SARS-CoV-2 RNA in wastewater matrix: a review.

SARS-CoV-2 is the agent responsible for the global pandemic sickness, COVID-19. It is an enveloped virus that belongs to the family Coronaviridae. Recent studies have revealed the fecal shedding of the virus and have been found to enter wastewater and aquatic systems. Prolonged viral presence in fecal samples is a common observation in the reported literature. Survival of the virus in the recipient environment could be a crucial factor that influences its fecal-oral transmission. The detection of a novel coronavirus in wastewater opportunity has potential for environmental surveillance at the community or population level. Such a surveillance system can enable the early detection of disease outbreaks in zones with pre-symptomatic/asymptomatic patients and act as a complementary tool for continuous monitoring of quarantine zones. In contrast to developed regions, resource constraints in underdeveloped communities coupled with different sanitation settings may pose a challenge to wastewater sampling and surveillance. To begin, this review summarizes the literature on the presence of SARS-CoV-2 in feces. The approaches for viral extraction, concentration, and detection in wastewater matrices are then highlighted. Finally, investigations on wastewater-based epidemiology for SARS-CoV-2 surveillance are reviewed.

Morphological and structural characterization of chitin as a substrate for the screening, production, and molecular characterization of chitinase by Bacillus velezensis.

The processing of shellfishery industrial wastes is gaining much interest in recent times due to the presence of valuable components. Chitin is one of the valuable components and is insoluble in most common solvents including water. In this study, a novel gram-positive bacterial strain capable of solubilizing chitin was screened from a prawn shell dumping yard. The chitinolytic activity of the isolated strain was observed through the zone of hydrolysis plate assay. The hyper-producing isolate was identified as Bacillus velezensis through the 16S rRNA sequencing technique. The structural and morphological characterization of raw and colloidal chitin preparation was carried out using FTIR, XRD, and SEM analysis. The residual protein and mineral content, degree of polymerization, and degree of acetylation were reported for both raw and colloidal chitin preparations. There was a linear increase in the chitinase activity with an increase in the colloidal chitin concentration. The maximum activity of chitinase was observed as 38.98 U/mL for the initial colloidal chitin concentration of 1.5%. Supplement of additional carbon sources, viz., glucose and maltose, did not improve the production of chitinase and resulted in a diauxic growth pattern. The maximum chitinase activity was observed to be 33.10 and 30.28 U/mL in the colloidal chitin-containing medium with and without glucose as a secondary carbon source, respectively. Interestingly, the addition of complex nitrogen sources has increased the production of chitinase. A 1.95- and 2.14-fold increase in the enzyme activity was observed with peptone and yeast extract, respectively. The chitinase was confirmed using SDS-PAGE, native PAGE, and zymograms. The optimum pH and temperature for chitinase enzyme activity were found to be 7.0 and 44 °C, respectively.

Transformation and fate of urea in pit-toilet blackwater after discharge to environment.

Studies on urea transformation reactions in blackwater are limited as urea rapidly hydrolyses under anaerobic condition. Since ammonium content of blackwater mainly originates from urine-urea, studying urea hydrolysis reactions is important to predict potential nitrate loads in aquifers from on-site sanitation facilities. In this study, urea spiked blackwater samples from pour flush pit toilet are used to examine the urea-ammonium pathways at varying initial urea concentrations and temperature. Based on laboratory results, the annual nitrate load imposed by the urea constituent of blackwater in a hard rock aquifer is predicted. Laboratory results illustrated that experimental temperature of 37 °C and pH range of 6.7 to 8.1 facilitated optimum urease enzyme activity at the initial substrate concentration of 500 mg/L. The Q10 value for urea transformation reactions indicated that increase in temperature has positive influence on enzyme activity. The reduction in urea concentration with time followed first-order kinetics. Part of ammonium ions in blackwater oxidises as nitrate ions that travel to the aquifer. Upon mixing and dilution, the nitrate concentration in 1 km2 of hard rock aquifer would annually increase by 0.004 mg/L due to blackwater infiltration from single household pour flush toilet.