Gallium-based liquid metals as smart responsive materials: Morphological forms and stimuli characterization.

Gallium-based liquid metals (GaLMs) have garnered monumental attention from the scientific community due to their diverse actuation characteristics. These metals possess remarkable characteristics, including high surface tension, excellent electrical and thermal conductivity, phase transformation behaviour, minimal viscosity and vapour pressure, lack of toxicity, and biocompatibility. In addition, GaLMs have melting points that are either lower or near room temperature, making them incredibly beneficial when compared to solid metals since they can be easily deformed. Thus, there has been significant progress in developing multifunctional devices using GaLMs, including bio-devices, flexible and self-healing circuits, and actuators. Despite numerous reports on these liquid metals (LMs), there is an urgent need for consolidated and coherent literature regarding their actuation principles linked to the targeted application. This will ensure that the reader gets the flavour of physics behind the actuation mechanism and how it can be utilized in diverse fields. Moreover, the actuation mechanism has been scattered in the literature, and thus, the primary motive of this review is to provide a one-stop solution for the actuation mechanism and the associated dynamics while directing the readers to specialized literature. Thus, addressing this issue, we thoroughly examine and present a detailed account of the actuation mechanisms of GaLMs while highlighting the science behind them. We also discuss the various morphologies of GaLMs and their crucial physical characteristics which decide their targeted application. Furthermore, we also delve into commonly held beliefs about GaLMs in the literature, such as their toxicity and antibacterial properties, to offer readers a more accurate understanding. Finally, we have explored several key unanswered aspects of the LM that should be explored in future research. The core strength of this review lies in its simplistic approach in offering a starting point for researchers venturing this innovative field, while we make use of existing literature to develop a comprehensive understanding.

Net Unidirectional Fluid Transport in Locally Heated Nanochannel by Thermo-osmosis.

Thermo-osmosis driven by temperature gradients generally requires two liquid reservoirs at different temperatures connected by porous bodies or capillaries. We demonstrate, by molecular dynamics simulation, a new phenomenon toward nanoscale thermo-osmosis. Upon heating at a certain region of a nanochannel, multiple nanoscale convective layers are formed and can be manipulated to generate a net fluid transport from one reservoir to another, even without a temperature difference between them. A net unidirectional fluid transport with different rates can be achieved by precisely controlling location of the heated region. The net fluid transport can be enhanced further by tuning liquid-wall interactions. The demonstrated phenomenon provides a strategy for enhancing fluid mixing, which is often inefficient in nanoscale flows. Our finding is promising for chip-level cooling. The heat generated by chips can be employed to produce asymmetric temperature gradients in channels through proper configuration. Coolant liquids can thus be circulated without extra pumps.

Picoliter agar droplet breakup in microfluidics meets microbiology application: numerical and experimental approaches.

Droplet microfluidics has provided lab-on-a-chip platforms with the capability of bacteria encapsulation in biomaterials, controlled culture environments, and live monitoring of growth and proliferation. The droplets are mainly generated from biomaterials with temperature dependent gelation behavior which necessitates stable and size-controlled droplet formation within microfluidics. Here, the biomaterial is agar hydrogel with a non-Newtonian response at operating temperatures below 40 °C, the upper-temperature threshold for cells and pathogens. The size of the produced droplets and the formation regimes are examined when the agar is injected at a constant temperature of 37 °C with agar concentrations of 0.5%, 1%, and 2% and different flow rate ratios of the dispersed phase to the continuous phase (φ: 0.1 to 1). The numerical simulations show that φ and the capillary number (Ca) are the key parameters controlling the agar droplet size and formation regime, from dripping to jetting. Also, increasing the agar concentration produces smaller droplets. The simulation data were validated against experimental agar droplet generation and transport in microfluidics. This work helps to understand the physics of droplet generation in droplet microfluidic systems operating with non-Newtonian fluids. Pathogenic bacteria were successfully cultured and monitored in high resolution in agar droplets for further research in antibiotic susceptibility testing in bacteremia and urinary tract infection.

Rapid and Highly Controlled Generation of Monodisperse Multiple Emulsions via a One-Step Hybrid Microfluidic Device.

Multiple Emulsions (MEs) contain a drop laden with many micro-droplets. A single-step microfluidic-based synthesis process of MEs is presented to provide a rapid and controlled generation of monodisperse MEs. The design relies on the interaction of three immiscible fluids with each other in subsequent droplet formation steps to generate monodisperse ME constructs. The design is within a microchannel consists of two compartments of cross-junction and T-junction. The high shear stress at the cross-junction creates a stagnation point that splits the first immiscible phase to four jet streams each of which are sprayed to micrometer droplets surrounded by the second phase. The resulted structure is then supported by the third phase at the T-junction to generate and transport MEs. The ME formation within microfluidics is numerically simulated and the effects of several key parameters on properties of MEs are investigated. The dimensionless modeling of ME formation enables to change only one parameter at the time and analyze the sensitivity of the system to each parameter. The results demonstrate the capability of highly controlled and high-throughput MEs formation in a one-step synthesis process. The consecutive MEs are monodisperse in size which open avenues for the generation of controlled MEs for different applications.

Passive microinjection within high-throughput microfluidics for controlled actuation of droplets and cells.

Microinjection is an effective actuation technique used for precise delivery of molecules and cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into single cells. Several microinjection techniques have been developed for actuating droplets and cells. However, they are still time-consuming, have shown limited success, and are not compatible with the needs of high-throughput (HT) serial microinjection. We present a new passive microinjection technique relying on pressure-driven fluid flow and pulsative flow patterns within an HT droplet microfluidic system to produce serial droplets and manage rapid and highly controlled microinjection into droplets. A microneedle is secured within the injection station to confine droplets during the microinjection. The confinement of droplets on the injection station prevents their movement or deformation during the injection process. Three-dimensional (3D) computational analysis is developed and validated to model the dynamics of multiphase flows during the emulsion generation. We investigate the influence of pulsative flows, microneedle parameters and synchronization on the efficacy of microinjection. Finally, the feasibility of implementing our microinjection model is examined experimentally. This technique can be used for tissue engineering, cells actuation and drug discovery as well as developing new strategies for drug delivery.

Dynamics of temperature-actuated droplets within microfluidics.

Characterizing the thermal behavior of dispersed droplets within microfluidic channels is crucial for different applications in lab-on-a-chip. In this paper, the physics of droplets volume during their transport over a heater is studied experimentally and numerically. The response of droplets to external heating is examined at temperature ranges of 25-90 °C and at different flow rates of the dispersed phase respect to the continuous flow. The results present a reliable prediction of the droplet volume and stability when heating is applied to the droplets at the downstream channel in a quite far distance from the droplets' ejection orifice. Increasing the ratio of flow rate resulted in larger droplets; for instance, the flow ratio of 0.25 produced drops with 40% larger diameter than the flow rate of 0.1. For every 10 °C increase in temperature of the droplets, the droplet diameter increased by about 5.7% and 4.2% for pure oil and oil with a surfactant, respectively. Also, the droplets showed a degree of instability during their transport over the heater at higher temperatures. Adding SPAN 20 surfactant improved the stability of the droplets at temperatures higher than 60 °C. The experimentally validated numerical model helped for systemic analysis of the influence of key temperature-dependence parameters (e.g. surface tension, density and viscosity of both phases) on controlling the volume and stability of droplets. Our findings supported to develop highly functional systems with a predetermined droplets performance under high temperatures up to 90 °C. This report provides a preliminary basis for enhancing the performance of droplet microfluidic systems for digital droplet polymerase chain reaction (ddPCR), continuous flow digital loop-mediated isothermal PCR (LAMP), and droplet-based antibiotic susceptibility testing.