Experimental infection in mice with Erwinia persicina.

Erwinia persicinus (E. persicina) is a plant pathogenic bacterial species that was previously isolated from a case of human infection. This study aimed to create an experimental infection protocol for E. persicina in laboratory mice. Seventy-two adult mice were divided into four groups (18 animal/group): the control group (G1), the group infected with E. persicina (G2), the group immune-suppressed with cyclophosphamide (G3) and the group immune-suppressed with cyclophosphamide and infected with E. persicina (G4). G2 and G4 were injected with 200 µL of (1 × 1013 cfu/ml) concentration intraperitoneally. Clinical signs, such as diarrhoea, apathy and mortality were observed only in G2 and G4 animals. E. persicina was not detected in blood. Necropsies of the G2 and G4 animals showed lesions in the intestine, liver, kidney and lung tissue. These lesions were characterized by infiltration of inflammatory cells, hyperaemia and focal areas of tissue necrosis in the liver. The results of the pro-inflammatory cytokines analysis revealed a significant increase in the levels of TNF-α and IL1-ß in the liver tissue of the G4 group. E. persicina is an emerging bacterium that can cause pathological lesions into mammalian tissue, which warrants further investigation.

Prevalence of Eimeria species among sheep and goats in Suez Governorate, Egypt.

Coccidiosis is a disease of high economic importance caused by Eimeria species that show ubiquitous distribution among several species including small ruminants. The prevalence of Eimeria infection in sheep and goats in Geneffe village, Suez Governorate, Egypt was determined during the period from March 2015 to February 2016. Total of 277 animals (142 sheep and 135 goats) were clinically examined and fecal samples were collected and tested both microscopically and by PCR. Sera samples of sheep and goats under 1 year were collected for biochemical analysis. Results revealed that (60%) of goats and (57.70%) of sheep were suffering from subclinical coccidiosis. Adult female goats were significantly (P < 0.05) more infected (82.2%) than adult male goats (40%). Eimeria infection was significantly prevalent in summer (75%) and autumn (74.2%) in sheep than winter (38.2%) and spring (43.2%), while goats did not show significant seasonal variations of infection. The Eimeria species were identified as E. crandallis, E. granulosa, E. ovina, E. parva, E. faurei, E. ovinoidalis, E intricate, E. pallida, E. arloingi, and E. ahasta in sheep, and E. ninakohlyakimovae, E. hirci, E. caprina, E. christenseni, E. jolchijevi, E. apsheronica and E. arloingi in goats. Although animals were subclinically infected with coccidia, some significant biochemical changes were observed in serum samples of sheep and goats. The molecular detection of Eimeria oocysts did not yield any positive results but after sporulation, Eimeria oocysts were detected at zone 100 bp. Our results showed a moderate prevalence of Eimeria infection among adult and yearling sheep and goats in Geneffe village. Suez governorate, Egypt.Hence, good control and prevention programs are necessary.