ABSTRACT
Application of hydrogels can be an effective technique in transferring the adipose-derived stem cells (ASCs) to injured tissue and their protection from further complications. Besides, acellular dermal matrix (ADM) has successfully been used in treatment of wounds. In this study, a combination of hylauronic acid (HA) and ASCs (HA/ASCs) was applied on burn wounds and the injured area was then covered by an ADM dressing in a rat model (ADM-HA/ASCs). Wound healing was evaluated by histopathological, histomorphometrical, molecular, biochemical, and scanning electron microscopy assessments on days 7, 14, and 28 post-wounding. ADM-HA/ASCs stimulated healing significantly more than the ADM-HA and ADM treated wounds, as it led to reduced inflammation, and improved angiogenesis and enhanced granulation tissue formation. Expression of interleukin-1ß (IL-1ß) and transforming growth factor-ß1 (TGF-ß1) was lower in the ADM-HA/ASCs treated wounds than the ADM-HA and ADM groups, at the seventh post-wounding day. ADM-HA/ASCs also enhanced the expression level of TGF-ß1 mRNA at 14 day post-wounding that was parallel to the experimental data from histological and biochemical assessments and confirmed the positive role of ASCs in repair of burn wounds. Additionally, increase in basic fibroblast growth factor (bFGF) expression and decreased TGF-ß1 level on the 28th post-wounding day indicated the anti-scarring activity of ASCs. HA loaded by adipose stem cells can represent a promising strategy in accelerating burn wound healing.
Subject(s)
Burns/therapy , Drug Carriers/chemistry , Hyaluronic Acid/chemistry , Hydrogels/chemistry , Wound Healing/drug effects , Acellular Dermis/metabolism , Adipocytes/cytology , Animals , Disease Models, Animal , Fibroblast Growth Factor 2/metabolism , Gene Expression Regulation , Humans , Hydrogels/metabolism , Hydroxyproline/chemistry , Interleukin-1beta/metabolism , Rats , Sheep , Skin , Stem Cell Transplantation , Stem Cells/metabolism , Transforming Growth Factor beta1/metabolismABSTRACT
ABSTRACT: FeNH4(SO4)2·12H2O (alum) efficiently catalyzes the one-pot three-component reaction of dimedone, aldehydes, and 3-aminocrotonate to afford 1,4-dihydropyridines. The work-up is easy, and the products are obtained in good to excellent yields and high purity.
ABSTRACT
A novel three-component, one-pot condensation of the zwitterion generated from dialkyl acetylenedicarboxylate and isocyanides with homophthalic anhydride is described. The reaction affords new 6-oxopyrano[2,3-c]isochromenes in good yield. Isochromenes have been reported to possess diverse biological activities such as antibacterial, antifungal, antiinflammatory, and antiangiogenic effects. Moreover, Theses important compounds are found in various natural products.
Subject(s)
Anti-Infective Agents/chemical synthesis , Benzopyrans/chemical synthesis , Combinatorial Chemistry Techniques/methods , Acetylene/analogs & derivatives , Acetylene/chemical synthesis , Acetylene/chemistry , Benzopyrans/chemistry , Cyanides/chemical synthesis , Cyanides/chemistry , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/chemistry , Molecular StructureSubject(s)
Abdomen , Foreign Bodies/surgery , Surgical Sponges , Humans , Male , Middle Aged , Time FactorsABSTRACT
[reaction: see text] 11-(1H-Pyrrol-1-yl)-11H-indeno[1,2-b]quinoxaline and 3-(1H-pyrrol-1-yl)indolin-2-one derivatives have been synthesized in good yields in a novel, one-pot, and efficient process by condensation of 11H-indeno[1,2-b]quinoxalin-11-one or isatin derivatives with 4-hydroxyproline on solid-support montmorillonite K10 under microwave irradiation.
ABSTRACT
KAl(SO(4))(2).12H(2)O is found to catalyze efficiently the stereoselective one-pot three-component cyclocondensation of homophthalic anhydride, aldehydes, and amines under mild conditions to afford the corresponding cis-isoquinolonic acids in good yields.
Subject(s)
Aluminum Compounds/chemistry , Isoquinolines/chemical synthesis , Potassium Compounds/chemistry , Sulfates/chemistry , Aldehydes/chemistry , Amines/chemistry , Anhydrides/chemistry , Catalysis , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , StereoisomerismABSTRACT
A new one-pot four component procedure for synthesis of densely functionalized pyrroles using commercially available ninhydrin with phosphorane intermediates produced in the reaction between triphenylphosphine, ammonium thiocyanate (or ammonium acetate) and various dialkyl acetylenedicarboxylates was developed.