Beneath the surface: Exploring microplastic intricacies in Anadara granosa.

Anadara granosa or blood cockles have been reported to be a candidate for biomonitoring agents due to their sedimentary nature and their nutrient uptake mechanisms. Yet, this bivalve is still regarded as a delicacy in Asian cuisine. Malaysia is the largest exporter of this sea product that contaminated cockles may also be experienced by the importing countries. However, the bioaccumulation of microplastics in A. granosa cultivated in Malaysia has not been extensively studied. It is crucial to comprehend the risk posed to humans by consuming A. granosa in their diet. Therefore, the purpose of this research is to investigate the levels of microplastic accumulation in A. granosa from major exporters in Peninsular Malaysia, to evaluate the associated risk of microplastics on the species, and to estimate daily human consumption of microplastics through the consumption of A. granosa. The abundance of microplastics was quantified through the use of a stereo microscope, and the polymer type was determined using FTIR and micro-FTIR. Findings from this investigation revealed that all samples of A. granosa were contaminated with microplastics, with the highest levels of accumulation found in bivalves collected from the west coast (0.26 ± 0.15 particles/g) of Peninsular Malaysia. Fragment and fiber microplastics, measuring between 0.05 and 0.1 mm in size, were found to be the most prevalent in A. granosa, with blue being the dominant identified colour and rayon being the most common polymer type. Microplastic risk assessment due to the presence of polyacrylate, polycarbonate (PC), and polymethyl methacrylate (PMMA) resulted in a high risk of contamination for A. granosa. It was further determined that the current estimated dietary intake (EDI) suggests that consumers of A. granosa uptake approximately 21.8-93.5 particles/person/year of microplastics. This study highlights that A. granosa accumulates microplastics, which could potentially result in bioaccumulation and biomagnification in humans through consumption.

Pasteurella multocida bacteremia: A case report of pelvic cavity inflammation with abnormal uterine bleeding, fever, and sclerotic bone lesions.

Background: Pasteurella multocida, is a bacterium that is frequently transmitted to humans from domestic pets. Infections are generally localized, but previous reports have exhibited that Pasteurella can cause systemic infections such as peritonitis, bacteremia and in some rare cases tubo-ovarian abscess. Case presentation: We present a case of a 46-year-old woman who came to the emergency department (ED) with complaints of pelvic pain, abnormal uterine bleeding (AUB), and fever. A non-contrast computed tomography (CT) of abdomen and pelvis showed uterine fibroids with sclerotic changes in lumbar vertebrae and pelvic bones causing a high degree of suspicion for cancer. On admission, blood culture, complete blood count (CBC) and tumor markers were drawn. Additionally, an endometrial biopsy was performed to rule out the possibility of endometrial cancer (EC). The patient underwent an exploratory laparoscopy with hysterectomy and bilateral salpingectomy. After diagnosis with P. multocida the patient was treated with five days of Meropenem. Conclusion: There are few cases of P. multocida peritonitis reported; in addition, findings of AUB with sclerotic bony changes in a middle-aged woman is often associated with EC. Thus, clinical suspicion from patient history, infectious disease work-up and diagnostic laparoscopy are essential for proper diagnosis and management.

Opportunistic Screening of Oral Potentially Malignant Disorders: A Public Health Need for India.

PURPOSE: Oral cancer (OC) is the leading cancer in 25% of Indian cancer registries, and 80% of OCs are diagnosed in advanced stages. OC screening is a topic of debate. Studies from other countries have used a variety of study designs as OC screening strategies. There are not many studies from India on strategic screening, and there is a need to review the literature to provide insights and knowledge about screening programs. The purpose of this narrative review is to present broad epidemiologic evidence on the OC burden in India, to discuss and summarize the currently available evidence for OC screening strategies, and to highlight a feasible opportunistic screening strategy for addressing OC burden in India. METHODS: Medline and EMBASE were used to identify articles. Data from GLOBOCAN and government reports were obtained from websites. As many key concepts and divergent views cannot be addressed with a single research question, a narrative review was considered appropriate, but to ensure a comprehensive literature search, a systematic review search strategy was used. RESULTS: OC rates are rising more rapidly in India than projected. Wide variations in OC incidence within India reflect regional diversity of risk factors. Studies abroad have demonstrated the feasibility of opportunistic screening of oral potentially malignant disorders by dentists; however, although recommendations exist in India, no studies of opportunistic screening by dentists have been reported. CONCLUSION: The projected major increases in the OC burden necessitate an OC screening program; opportunistic screening of high-risk groups by dentists using oral visual examination is recommended as a cost-effective strategy. As a way forward, a pilot project to assess the feasibility of regional opportunistic screening is in progress.

Worldwide distribution and abundance of microplastic: How dire is the situation?

The widespread occurrence of microplastic has invaded the environment to an extent that it appears to be present throughout the globe. This review investigated the global abundance and distribution of microplastics in marine and freshwater ecosystems. Furthermore, the issues and challenges have been addressed for better findings in microplastics studies. Findings revealed that the accumulation of microplastics varies geographically, with locations, hydrodynamic conditions, environmental pressure, and time. From this review, it is crucial that proper regulations are proposed and implemented in order to reduce the occurrence of microplastics in the aquatic environment. Without appropriate law and regulations, microplastic pollution will eventually threaten human livelihood.

Prognostic significance of tumour infiltrating B lymphocytes in breast ductal carcinoma in situ.

AIMS: Tumour-infiltrating lymphocytes (TILs) are an important component of the immune response to cancer and have a prognostic value in breast cancer. Although several studies have investigated the role of T lymphocytes in breast cancer, the role of B lymphocytes (TIL-Bs) in ductal carcinoma in situ (DCIS) remains uncertain. This study aimed to assess the role of TIL-Bs in DCIS. METHODS AND RESULTS: Eighty DCIS cases (36 pure DCIS and 44 mixed with invasive cancer) were stained immunohistochemically for B lineage markers CD19, CD20 and the plasma cell marker CD138. TIL-Bs density and localization were assessed, including relation to the in-situ and invasive components. An association with clinicopathological data and patient outcome was performed. Pure DCIS showed a higher number of TIL-Bs and lymphoid aggregates than DCIS associated with invasion. In pure DCIS, a higher number of peri- and paratumoral TIL-Bs was associated significantly with large tumour size (P = 0.016), hormone receptor (ER/PR) negative (P = 0.008) and HER2+ status (P = 0.010). In tumours with mixed DCIS and invasive components, cases with high-density B lymphocytes, irrespective of their location or topographic distribution, were associated significantly with variables of poor prognosis, including larger size, high grade, lymphovascular invasion, lymph node metastases, ER/PR-negative and HER2+ status. Outcome analysis showed that pure DCIS associated with higher numbers of B lymphocytes had shorter recurrence-free interval (P = 0.04); however, the association was not significant with the CD138+ plasma cell count (P = 0.07). CONCLUSION: Assessment of TIL-B cells based on location and topographic distribution can provide prognostic information. Validation in a larger cohort is warranted.

One-pot protection-glycosylation reactions for synthesis of lipid†II analogues.

(2,6-Dichloro-4-methoxyphenyl)(2,4-dichlorophenyl)methyl trichloroacetimidate (3) and its polymer-supported reagent 4 can be successfully applied to a one-pot protection-glycosylation reaction to form the disaccharide derivative 7 d for the synthesis of lipid II analogues. The temporary protecting group or linker at the C-6 position and N-Troc protecting group of 7 d can be cleaved simultaneously through a reductive condition. Overall yields of syntheses of lipid II (1) and neryl-lipid II N(ε)-dansylthiourea are significantly improved by using the described methods.

Structure-activity relationship studies of novel pyrazole and imidazole carboxamides as cannabinoid-1 (CB1) antagonists.

The synthesis and biological evaluation of novel pyrazole and imidazole carboxamides as CB1 antagonists are described. As a part of eastern amide SAR, various chemically diverse motifs were introduced on rimonabant template. The central pyrazole core was also replaced with its conformationally constrained motif and imidazole moieties. In general, a range of modifications were well tolerated. Several molecules with low- and sub-nanomolar potencies were identified as potent CB1 receptor antagonists. The in vivo proof of principle for weight loss is demonstrated with a lead compound in DIO mice model.

Novel pyrazole-3-carboxamide derivatives as cannabinoid-1 (CB1) antagonists: journey from non-polar to polar amides.

The synthesis and biological evaluation of novel pyrazole-3-carboxamide derivatives as CB1 antagonists are described. As a part of eastern amide SAR, various chemically diverse motifs were introduced. In general, a range of modifications were well tolerated. Several molecules with high polar surface area were also identified as potent CB1 receptor antagonists. The in vivo proof of principle for weight loss is exemplified with a lead compound from this series.