Impact of 28 year old agroforestry systems on soil carbon dynamics in Eastern Himalayas.

Globally, various estimates are available on the above-ground (plant parts) carbon (C) sequestering potential of agroforestry systems (AFSs). However, information on soil organic carbon (SOC) sequestration potential is limited for AFSs. Furthermore, the impacts of AFSs established for the restoration of C in degraded soils (prone to soil erosion, C and nutrients loss, etc.) of Himalayas are rarely investigated. Thus, a study was conducted on an agroforestry block established in 1989 at the Indian Council of Agricultural Research (ICAR), Research Complex for North Eastern Hill (NEH) Region, Lembucherra, Tripura, India. The AFSs comprised of four multipurpose tree species viz., teak (Tectona grandis Linn), sissoo (Dalbergia sissoo Roxb. Ex DC.), eucalyptus (Eucalyptus globulus L.), and neem (Azadirachta indica A. Juss) in combination with pineapple (Ananas comosus L. merr.). Planted in three times replicated randomized block design. After 28 years of establishment, the impacts of these AFSs were assessed on SOC stocks and its fraction pools. Results revealed that sissoo + pineapple system stored the highest SOC stocks in 0-15 cm (22.1 ± 1.4 Mg/ha) and 30-60 cm (18.0 ± 4.3 Mg/ha) depths, whereas the SOC stocks in 15-30 cm (12.2 ± 1.2 Mg/ha) and 0-30 cm (34.0 ± 1.6 Mg/ha) were the highest under teak + pineapple. When considering the entire 0-100 cm soil profile, the SOC stocks ranged between 65.3 and 71.6 Mg/ha across the diverse AFSs which was significantly higher than that under cultivated land (52.8 ± 2.6 Mg/ha). The sissoo + pineapple system had the highest SOC stock in 0-100 cm (71.6 ± 5.8 Mg/ha). The share of passive carbon (PC, less labile + non-labile) pools to SOC stocks under AFSs followed the order of sissoo + pineapple > teak + pineapple > neem + pineapple > eucalyptus + pineapple. The PC or recalcitrant pools of SOC stocks at 0-100 cm were 54.2-60.6% under various AFSs. Results revealed that the establishment of AFSs with pineapple on degraded lands increased a significant amount of C and had a considerable effect on soil quality in comparison to C present in soils under cropland. Thus, a large scale adoption of AFSs may restore C lost through the cultivation of the crop in degraded lands and provide a feasible option for livelihood through concurrent cultivation of multipurpose tree species and agri-horticulture crops.

Impact of land configuration and organic nutrient management on productivity, quality and soil properties under baby corn in Eastern Himalayas.

Appropriate land configuration and assured nutrient supply are prerequisites for quality organic baby corn (Zea mays L.) production in high rainfall areas of the delicate Eastern Himalayan Region of India. A long term (5-year) study was conducted during 2012-2016 on a sandy loam soil in the mid attitude of Sikkim, Eastern Himalayan Region of India to evaluate the productivity, produce quality, the profitability of baby corn, and soil properties under different land configurations comprising flatbed, ridge and furrow, and broad bed and furrow, and organic nutrient management practices comprising un-amended control, farmyard manure 12 t ha-1, vermicompost 4 t ha-1 and farmyard manure 6 t ha-1 + vermicompost 2 t ha-1. The baby corn sown on broad bed and furrow had the tallest plant (149.25 cm), maximum dry matter (64.33 g plant-1), highest leaf area index (3.5), maximum cob length (8.10 cm), cob girth (6.13 cm) and cob weight (8.14 g) leading to significantly higher fresh baby corn yield (1.89 t ha-1), and net returns (US$ 906.1 ha-1) than those of other treatments. Mineral composition (phosphorus, potassium, iron, and zinc), protein, and ascorbic acid content were also the highest in baby corn grown under the broad bed and furrow system. The soil of broad bed and furrow had a higher pH, organic carbon content, organic carbon pools, microbial biomass carbon, and enzymatic activities (dehydrogenase, fluorescein diacetate, and acid phosphatase) compared to soils of other land configurations. A combined application of farmyard manure (6 t ha-1) + vermicompost (2 t ha-1) improved the crop growth and produced 117.8% higher fresh baby corn and 99.7% higher fodder yield over control (0.9 t fresh corn and 13.02 t fodder yield ha-1), respectively. This treatment also registered significantly higher gross return (US$ 1746.9 ha-1), net return (US$ 935.8 ha-1), and benefit-cost ratio (2.15) than other nutrient management practices. Fresh cob quality in terms of protein (22.91%) and ascorbic acid content (101.6 mg 100 g-1) was observed to be significantly superior under combined application of farmyard manure (6 t ha-1) + vermicompost (2 t ha-1) than those of other nutrient management systems. However, fresh baby corn cobs produced with vermicompost 4 t ha-1 had the highest concentration of phosphorus, potassium, iron, and zinc. Application of farmyard manure 12 t ha-1 registered the maximum increment in soil organic carbon content (1.52%), its pool (40.6 t ha-1) and carbon sequestration rate (0.74 t ha-1 year-1) followed by integrated application of farmyard manure (6 t ha-1) and vermicompost (2 t ha-1). The maximum soil microbial biomass carbon and enzymatic activities [dehydrogenase (22.1 µg TPF g-1 soil h-1) and fluorescein diacetate (67.1 µg FDA g-1 soil h-1)] were noted with the combined use of farmyard manure (6 t ha-1) + vermicompost (2 t ha-1). Thus, the study suggests that the broad bed and furrow land configuration along with the combined application of farmyard manure + vermicompost could be an economically feasible practice for quality organic baby corn production and soil health improvement in the Eastern Himalaya and other similar eco-regions elsewhere.

Designing energy-efficient, economically sustainable and environmentally safe cropping system for the rainfed maize-fallow land of the Eastern Himalayas.

Achieving a circular economic model in agriculture and meeting the food requirement of the growing population is a global challenge. The task is much more daunting in the Eastern Himalaya where low productive maize-fallow is a predominant production system. To enhance system productivity and energy use efficiency while maintaining environmental sustainability and economic profitability, therefore, energy-efficient, low carbon footprint (CF; CO2-e) and profitable short duration crops must be made an integral part of the maize fallow system. Thus, six cropping systems viz., maize-fallow, maize-French bean, maize-soybean, maize-black gram, maize-green gram, and maize-toria were evaluated for seven consecutive years (2011-2018) to assess their energy requirement and efficiency, carbon footprint (CF; CO2-e), economic returns and eco-efficiency. The results revealed that the maize-French bean system had the highest system productivity (11.4 Mg ha-1), energy productivity (17.9), energy profitability (15.9) and non-renewable energy use efficiency (9.97). The maize-French bean system had also the highest net profit (US$ 3764.5 ha-1) and benefit to cost ratio (2.54). The energy consumed under different inputs/activities across the cropping systems for chemical fertilizers, diesel and machinery ranged from 50.0-62.7%, 17.3-20.8% and 4.6-15.4%, respectively. The maize-fallow system had the highest CF (0.34 kg CO2 e per kg grain) while, the maize-French bean system had the lowest CF (0.19 kg CO2 e per kg grain). The maize-French bean system had also considerably increased eco-efficiency both in terms of energy use (US$ 0.23 MJ-1) and (US$ 1.78 per kg CO2 e) over maize-fallow system. Thus, the study has suggested that maize-French bean system is energy-efficient, economically viable and environmentally safer systems to utilize maize fallow and improve food security, may help in achieving green/circular economy.

Unusual presentation of follicular carcinoma thyroid with special emphasis on their management.

INTRODUCTION: Follicular carcinoma of thyroid usually behaves in an indolent manner with low metastatic potential. Distant metastases as initial presentation is rare in follicular carcinoma; especially in young patients. PRESENTATION OF CASE: We report the clinical, pathological features and the management of three different cases of follicular carcinoma of the thyroid with unusual presentations at the time of diagnosis. First case presented as thyroid abscess, second case with a large skull swelling in a pre-exiting goiter and the third case with a swelling in the sternum. DISCUSSION: Follicular carcinoma of thyroid is the second category of well-differentiated thyroid cancer that constitutes about 10% of all thyroid malignancies. Blood borne metastasis is common with spread to lung, bone and other solid organs. In less than 10% cases of follicular carcinoma, there is evidence of lymphatic involvement. The patients' presentations above are highly unusual. CONCLUSION: Recognizing these cases has a significant impact on clinical decision-making and prognosis of the patients. Treatment in these patients should be individualized and an alternative therapeutic approach should be considered.

First Record of Rhizoctonia solani AG 1-IB on Mucuna pruriens in India.

Mucuna is the source of L-Dopa (L 3,4 dihydroxyphenylalanine), a precursor of a dopamine used to treat Parkinson's disease. Leaf blight symptoms were observed on Mucuna pruriens plants in October to November 2010 in a field at the Indian Council of Agricultural Research Complex for the Northeastern Hill Region, Umiam, Meghalaya, India. Symptoms included black necrotic areas on leaves, collapsed leaf tissue and, occasionally, fungal growth visible on the leaf. In advanced infections, dead leaves were attached to the stem, followed by defoliation with only infected pods still attached. Approximately 10% of plants were infected in ~0.5 ha surveyed. Symptomatic leaf pieces were washed with sterile water, surface-sterilized using 4% NaOCl for 30 s, washed again, blotted dry, and plated on PDA amended with streptomycin (100 ppm). Characteristics of three fungal isolates were typical of Rhizoctonia solani J.G. Kühn [teleomorph = Thanatephorus cucumeris (A. B. Frank) Donk], i.e., hyphal branching at 90° angles, basal constriction at the hyphal branching point with a septum close to the lateral hyphum (3), and presence of multinucleate hyphal cells confirmed using DAPI (2-(4-amidinophenyl)-1H-indole-6-carboxamidine) staining (1). A culture was deposited at the Agharkar Research Institute, Pune, India (NFCCI No. 2602). The ITS1-5.8S-ITS2 region of nuclear rDNA of one isolate was sequenced after amplification with primers ITS1 and ITS4 (4), (GenBank Accession No. JQ675536). A BLAST search revealed 99% similarity of the sequence with that of 2 R. solani AG 1-IB isolates (AB122137 and AB000039). Sequences were aligned using MAFT Version 6. Maximum parsimony analysis using MEGA 5 placed the test isolate in AG 1-IB with 99% bootstrap support. PCR assays with primers for R. solani AG 1-IB produced a DNA band of ~300 bp (2). Koch's postulates were completed by inoculating 5-mm colonized plugs of PDA at the soil line of each of 5 potted, 40-day-old plants of M. pruriens, and covering the base of each plant with moistened cheesecloth. In addition, 3 plants were inoculated with colonized plugs at the junction of the lamina and petiole of 9 leaves/plant, spraying the plants with sterilized water, and covering the plants with polythene for 3 days. In addition, 10 detached leaves were inoculated with colonized PDA plugs and incubated in a moist chamber. Three non-inoculated plants served as a control treatment for the first 2 methods, and 10 leaves as a control treatment for the third method with sterilized PDA plugs. Symptoms of leaf blight (necrosis from base to leaf tip, with abundant fungal growth) developed in 6 to 7 days on plants inoculated at the soil line, 4 days on leaves inoculated at the junction of the lamina and petiole, and 2 to 3 days on detached leaves. Control plants and leaves remained asymptomatic for all 3 methods. R. solani was reisolated from inoculated plants as described above, and confirmed to be AG 1-IB. The fungus was not reisolated from control plants or leaves. To our knowledge, this is the first record of R. solani AG 1-IB causing leaf blight on M. pruriens in India. References: (1) M. M. Kulik and P. D. Dery. Biotech. Histochem. 70:95, 1995. (2) M. Matsumoto. Mycoscience 43:185, 2002. (3) B. Sneh et al. Identification of Rhizoctonia Species. The American Phytopathological Society Press, St Paul, MN, 1991. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, San Diego, CA, 1990.

Clinical effect of Kukkutanda Twak Bhasma in the management of Swetapradara.

Swetapradara is an important gynecological disorder nowadays. Most women in the reproductive age group complain about white discharge. Due to white discharge, they are prone to so many other symptoms, such as backache, itching in vulva, and burning micturition. According to Ayurveda, swetapradara is caused by the vitiation of Kapha and Vata dosha. Kukkutanda twak is also said to be Kapha Vata shamaka and swetapradara shamaka. In Ayurveda so many drugs are mentioned in the treatment of swetapradara. Among them Kukkutanda twak bhasma is a good medicine. In this clinical study Kukkutanda twak bhasma has shown statistically significant improvement in white discharge, backache, itching, anemia, weakness, and urinary tract infection.

Reinforcement of endocrine surgery training: impact of telemedicine technology in a developing country context.

AIMS AND OBJECTIVES: Technology-based outreach programs for knowledge sharing and skill development using telemedicine is a novel approach to developing subspecialties in regions where there is a lack of specialists and dedicated departments. Endocrine surgery is one such emerging subspecialty. There are few dedicated surgeons and centers even in the developed world. India has only one such center. The aim of this study was to assess the feasibility and impact of telemedicine on the reinforcement of endocrine surgery training at distant places to develop this subspecialty in a developing country such as India. MATERIAL AND METHODS: Two faculty members from the General Surgery Department at SCB Medical College in Cuttack, Orissa, India registered at Sanjay Gandhi Postgraduate Institute of Medical Sciences in Lucknow for a short 3-month training course in endocrine surgery in 1998 and thereafter consulted through telephone calls and e-mail when required. Telemedicine-based mentoring was introduced in 2001 as a reinforcement method for continuing training and skills development. Various training modules were used, including tele-consultation, case presentation, treatment planning, tele-radiology, tele-pathology, and tele-continuing medical education (CME) programs and workshops. The outcome was assessed in terms of the increase in the number of patients with endocrine disorders seen and operated on by trainees and the complication rates. RESULTS: A total of 70 telemedicine sessions were held between 2001 and 2005. They included tele-education and surgical treatment planning (n = 44), tele-consultation including tele-pathology and tele-radiology (n = 26), and tele-surgical conferences/CME (n = 6). The number of endocrine surgical cases performed by trainees after training (phase II) increased significantly compared to those before training (phase I), with a further increase after starting telemedicine-enabled mentoring (phase III). Most of the patients operated on by the trainees had thyroid problems, although these surgeons started operating on a few patients with parathyroid and adrenal disorders as well. The morbidity figures for the thyroidectomy operations (including total thyroidectomy) were comparable to those at the training institution. CONCLUSION: The concept of training a group of motivated general surgeons to practice safe endocrine surgery at a remote center via telemedicine-aided reinforcement of their training is thus feasible. This is particularly true in the case of thyroidectomy operations, which constitute the major workload of endocrine surgery in our country. The same program might be applicable to the development of other medical subspecialties in a developing country as well.

Toxicity of two carbamate insecticides to the cyanobacterium Anabaena PCC 7120 and computations of partial lethal concentrations by the probit method.

Toxicity studies of two commercial carbamate insecticides, carbaryl and carbofuran with the nitrogen-fixing filamentous cyanobacterium Anabaena PCC 7120, are described. Under nitrogen-fixing conditions and with calcium nitrate supplementation, 100 and 120 ppm carbaryl were the respective lethal concentrations (LC100), while 20 to 80 ppm (nitrogen-fixing conditions) and 20 to 100 ppm (with nitrate supplementation) were the partial lethal doses (

Cryptococcus neoformans meningitis: a case report.

A 4-year-old Motswana boy suffered from cryptococcal meningitis; in addition to the signs of meningeal irritation, he had cortical blindness and seventh cranial nerve palsy. The child improved after treatment with amphotericin B and 5-fluorocytosine. It is suggested that this combination may be better than either drug used alone.