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1.
Mol Biotechnol ; 57(11-12): 1003-9, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26438488

ABSTRACT

Superoxide dismutase (SOD) detoxifies cell-toxic superoxide radicals and constitutes an important component of antioxidant machinery in aerobic organisms, including cyanobacteria. The iron-containing SOD (SodB) is one of the most abundant soluble proteins in the cytosol of the nitrogen-fixing cyanobacterium Nostoc punctiforme ATCC 29133, and therefore, we investigated its biochemical properties and response to oxidative stress. The putative SodB-encoding open reading frame Npun_R6491 was cloned and overexpressed in Escherichia coli as a C-terminally hexahistidine-tagged protein. The purified recombinant protein had a SodB specific activity of 2560 ± 48 U/mg protein at pH 7.8 and was highly thermostable. The presence of a characteristic iron absorption peak at 350 nm, and its sensitivity to H2O2 and azide, confirmed that the SodB is an iron-containing SOD. Transcript level of SodB in nitrogen-fixing cultures of N. punctiforme decreased considerably (threefold) after exposure to an oxidative stress-generating herbicide methyl viologen for 4 h. Furthermore, in-gel SOD activity analysis of such cultures grown at increasing concentrations of methyl viologen also showed a loss of SodB activity. These results suggest that SodB is not the primary scavenger of superoxide radicals induced by methyl viologen in N. punctiforme.


Subject(s)
Bacterial Proteins/metabolism , Nostoc/enzymology , Oxidative Stress/drug effects , Paraquat/toxicity , Superoxide Dismutase/metabolism , Bacterial Proteins/genetics , Cloning, Molecular , Computational Biology , Free Radical Scavengers/metabolism , Herbicides/toxicity , Hydrogen-Ion Concentration , Nostoc/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Superoxide Dismutase/genetics
2.
Appl Microbiol Biotechnol ; 98(8): 3809-18, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24384747

ABSTRACT

A spontaneous methyl viologen (MV)-resistant mutant of the nitrogen-fixing cyanobacterium Nostoc punctiforme ATCC 29133 was isolated and the major enzymatic antioxidants involved in combating MV-induced oxidative stress were evaluated. The mutant displayed a high constitutive catalase activity as a consequence of which, the intracellular level of reactive oxygen species in the mutant was lower than the wild type (N. punctiforme) in the presence of MV. The superoxide dismutase (SOD) activity that consisted of a SodA (manganese-SOD) and a SodB (iron-SOD) was not suppressed in the mutant following MV treatment. The mutant was, however, characterised by a lower peroxidase activity compared with its wild type, and its improved tolerance to externally added H2O2 could only be attributed to enhanced catalase activity. Furthermore, MV-induced toxic effects on the wild type such as (1) loss of photosynthetic performance assessed as maximal quantum yield of photosystem II, (2) nitrogenase inactivation, and (3) filament fragmentation and cell lysis were not observed in the mutant. These findings highlight the importance of catalase in preventing MV-promoted oxidative damage and cell death in the cyanobacterium N. punctiforme. Such oxidative stress resistant mutants of cyanobacteria are likely to be a better source of biofertilisers, as they can grow and fix nitrogen in an unhindered manner in agricultural fields that are often contaminated with the herbicide MV, also commonly known as paraquat.


Subject(s)
Catalase/metabolism , Drug Resistance, Bacterial , Nostoc/enzymology , Nostoc/metabolism , Oxidative Stress , Paraquat/metabolism , Paraquat/toxicity , Cytosol/chemistry , Hydrogen Peroxide/metabolism , Hydrogen Peroxide/toxicity , Nostoc/growth & development , Nostoc/isolation & purification , Reactive Oxygen Species/analysis
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