Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
STAR Protoc ; 4(1): 101930, 2023 03 17.
Article in English | MEDLINE | ID: mdl-36520626

ABSTRACT

Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of interest. We detail steps to use an open access web app to automate the design of mutagenesis primers optimized for our cloning protocols in a 96-well plate format. The protocol allows most molecular biology labs to clone 96 mutants (from PCR to sequence ready plasmid) in 3 days.


Subject(s)
Polymerase Chain Reaction , Polymerase Chain Reaction/methods , Gene Library , Mutagenesis , Mutagenesis, Site-Directed , Cloning, Molecular
SELECTION OF CITATIONS
SEARCH DETAIL
...