Subsurface fracturing of sedimentary stones caused by bullet impacts.

The immovable nature of built heritage means that it is particularly vulnerable during times of armed conflict. Although impacts from small arms and shrapnel leave relatively inconspicuous impact scars, they elevate the risk of future stone deterioration. This study investigates the subsurface damage caused by bullet impacts, which is not apparent from surface inspection, in order to better understand the geometry and mechanics of this form of conflict damage to heritage. Controlled firearm experiments were conducted to simulate conflict damage to sandstone and limestone buildings. The bullet impacts created conical fractures or zones of increased fracture intensity below the impact, radial fractures, and spallation, in addition to a crater. Dynamic fracture distinguishes the formation of these features from quasi static cone crack experiments, while the lack of a shockwave differentiates these bullet impacts from hypervelocity experiments. Damage was created by momentum transfer from the bullet, so that differences in target properties had large effects on the nature of the damage. The crater in the limestone target was almost an order of magnitude deeper than the sandstone crater, and large open fractures formed in the limestone below the crater floor, compared with zones of increased fracture intensity in the sandstone target. Microstructural analysis of subsurface damage showed that fracture intensity decreased with increasing distance from the impact centre, suggesting that regions proximal to the impact are at increased risk of future deterioration. Conical subsurface fractures dipping away from the impact beneath multiple impact craters could link up, creating a continuous fracture network. By providing pathways for moisture and other weathering agents, fractures enlarge the region at increased risk of deterioration. Their lack of surface expression makes understanding their formation a vital part of future surveying and post conflict assessments.

Structural Evolution of Gene Promoters Driven by Primate-Specific KRAB Zinc Finger Proteins.

Krüppel-associated box (KRAB) zinc finger proteins (KZNFs) recognize and repress transposable elements (TEs); TEs are DNA elements that are capable of replicating themselves throughout our genomes with potentially harmful consequences. However, genes from this family of transcription factors have a much wider potential for genomic regulation. KZNFs have become integrated into gene-regulatory networks through the control of TEs that function as enhancers and gene promoters; some KZNFs also bind directly to gene promoters, suggesting an additional, more direct layer of KZNF co-option into gene-regulatory networks. Binding site analysis of ZNF519, ZNF441, and ZNF468 suggests the structural evolution of KZNFs to recognize TEs can result in coincidental binding to gene promoters independent of TE sequences. We show a higher rate of sequence turnover in gene promoter KZNF binding sites than neighboring regions, implying a selective pressure is being applied by the binding of a KZNF. Through CRISPR/Cas9 mediated genetic deletion of ZNF519, ZNF441, and ZNF468, we provide further evidence for genome-wide co-option of the KZNF-mediated gene-regulatory functions; KZNF knockout leads to changes in expression of KZNF-bound genes in neuronal lineages. Finally, we show that the opposite can be established upon KZNF overexpression, further strengthening the support for the role of KZNFs as bona-fide gene regulators. With no eminent role for ZNF519 in controlling its TE target, our study may provide a snapshot into the early stages of the completed co-option of a KZNF, showing the lasting, multilayered impact that retrovirus invasions and host response mechanisms can have upon the evolution of our genomes.

Bullet impacts in building stone excavate approximately conical craters, with dimensions that are controlled by target material.

Bullet impacts are a ubiquitous form of damage to the built environment resulting from armed conflicts. Bullet impacts into stone buildings result in surficial cratering, fracturing, and changes to material properties, such as permeability and surface hardness. Controlled experiments into two different sedimentary stones were conducted to characterise surface damage and to investigate the relationship between the impact energy (a function of engagement distance) and crater volumes. Simplified geometries of crater volume using only depth and diameter measurements showed that the volume of a simple cone provides the best approximation (within 5%) to crater volume measured from photogrammetry models. This result suggests a quick and efficient method of estimating crater volumes during field assessments of damage. Impact energy has little consistent effect on crater volume over the engagement distances studied (100-400 m), but different target materials result in an order of magnitude variation in measured crater volumes. Bullet impacts in the experiments are similar in appearance to damage caused by hypervelocity experiments, but crater excavation is driven by momentum transfer to the target rather than a hemispherical shock wave. Therefore in contrast to predictions of impact scaling relationships for hypervelocity experiments, target material plays the dominant role in controlling damage, not projectile energy.

Surface damage from perpendicular and oblique bullet impacts in stone.

Controlled experiments were conducted to investigate the surface damage caused by perpendicular and oblique impacts of bullets into sandstone and limestone targets. Individual bullets fired in conditions simulating modern rifles at typical combat distances excavated craters with diameters from 22 to 74 mm and depths from 3 to 24 mm. Limestone target craters were up to twice as large and deep as those in sandstone. These craters have a complex shape consisting of a central excavation surrounded by a shallow dish, compared to the simple bowl shape of most sandstone impacts. Radial fractures extending to the edge of the target block were common in limestone targets. Impacts at an angle of 45° to the surface in both rock types result in asymmetric craters. Two common types of intermediate cartridge (ammunition) were compared: the steel-tipped 5.56 × 45 mm NATO projectile generally produced larger and deeper craters than the 7.62 × 39 mm projectile that is commonly fired from AK-47 rifles, despite having approximately half the mass of the latter. These results characterize the sort of damage that can be expected at many sites of cultural significance involved in contemporary conflict zones, and have important implications for their conservation: for example building stone with low tensile strength is likely to sustain more damage and be at risk of greater deterioration.

Sediment source fingerprinting: benchmarking recent outputs, remaining challenges and emerging themes.

PURPOSE: This review of sediment source fingerprinting assesses the current state-of-the-art, remaining challenges and emerging themes. It combines inputs from international scientists either with track records in the approach or with expertise relevant to progressing the science. METHODS: Web of Science and Google Scholar were used to review published papers spanning the period 2013-2019, inclusive, to confirm publication trends in quantities of papers by study area country and the types of tracers used. The most recent (2018-2019, inclusive) papers were also benchmarked using a methodological decision-tree published in 2017. SCOPE: Areas requiring further research and international consensus on methodological detail are reviewed, and these comprise spatial variability in tracers and corresponding sampling implications for end-members, temporal variability in tracers and sampling implications for end-members and target sediment, tracer conservation and knowledge-based pre-selection, the physico-chemical basis for source discrimination and dissemination of fingerprinting results to stakeholders. Emerging themes are also discussed: novel tracers, concentration-dependence for biomarkers, combining sediment fingerprinting and age-dating, applications to sediment-bound pollutants, incorporation of supportive spatial information to augment discrimination and modelling, aeolian sediment source fingerprinting, integration with process-based models and development of open-access software tools for data processing. CONCLUSIONS: The popularity of sediment source fingerprinting continues on an upward trend globally, but with this growth comes issues surrounding lack of standardisation and procedural diversity. Nonetheless, the last 2 years have also evidenced growing uptake of critical requirements for robust applications and this review is intended to signpost investigators, both old and new, towards these benchmarks and remaining research challenges for, and emerging options for different applications of, the fingerprinting approach.

The benefit of a tough skin: bullet holes, weathering and the preservation of heritage.

Projectile damage to building stone is a widespread phenomenon. Sites damaged 100 years ago during the First World War still see daily use, while in a more contemporary setting numerous reports show the damage to buildings in Babylon, Mosul and Palmyra. While research has been carried out on the long-term effects of conflict such as fire damage, little is known about the protracted damage sustained through the impact of bullets, shrapnel and other metal projectiles outside of the field of engineering focused on ceramics and metals. To investigate alterations to mineral structure caused by projectile damage, impacts were created in medium-grained, well-compacted, mesoporous sandstone samples using 0.22 calibre lead bullets shot at a distance of 20 m. Half these samples were treated with a surface consolidant (Wacker OH 100), to mimic natural cementation of the rock surface. These samples were then tested for changes to surface hardness and moisture movement during temperature cycles of 15-65°C. Petrographic thin section analysis was carried out to investigate the micro-scale deformation associated with high-speed impact. The results surprisingly show that stress build-up behind pre-existing cementation of the surface, as found in heritage sites that have been exposed to moisture and temperature fluctuations for longer periods of time, can be alleviated with a bullet impact. However, fracture networks and alteration of the mineral matrices still form a weak point within the structure, even at a relatively low impact calibre. This initial study illustrates the need for geomorphologists, geologists, engineers and heritage specialists to work collectively to gain further insights into the long-term impact of higher calibre armed warfare on heritage deterioration.

Photoacclimation by Arctic cryoconite phototrophs.

Cryoconite is a matrix of sediment, biogenic polymer and a microbial community that resides on glacier surfaces. The phototrophic component of this community is well adapted to this extreme environment, including high light stress. Photoacclimation of the cryoconite phototrophic community on Longyearbreen, Svalbard, was investigated using in situ variable chlorophyll fluorescence. Rapid light curves (RLCs) and induction-recovery curves were used to analyse photosystem II quantum efficiency, relative electron transport rate and forms of downregulation including non-photochemical quenching (NPQ) and state transitions in cyanobacteria. Phototrophs used a combination of behavioural and physiological photochemical downregulation. Behavioural downregulation is hypothesised to incorporate chloroplast movement and cell or filament positioning within the sediment matrix in order to shade from high light, which resulted in a lack of saturation of RLCs and hence overestimation of productivity. Physiological downregulation likely consisted of biphasic NPQ, comprising a steadily induced light-dependent form and a light-independent NPQ that was not reversed with decreasing light intensity. State transitions by cyanobacteria were the most likely physiological downregulation employed by cyanobacteria within the mixed phototroph community. These findings demonstrate that cryoconite phototrophs combine multiple forms of physiological and behavioural downregulation to optimise light exposure and maximise photosynthetic productivity. This plasticity of photoacclimation enables them to survive productively in the high-light stress environment on the ice surface.