Prime solutions for cardiopulmonary bypass in neonates: antioxidant capacity of prime based on albumin or fresh frozen plasma.

OBJECTIVE: Oxidative damage and inflammation are believed to play an important role in postoperative complications after cardiopulmonary bypass. During bypass, a prime solution with a high antioxidant capacity may reduce the oxidative damage and inflammation. We investigated total antioxidant capacity and individual scavengers during the preparation of 2 different prime solutions. METHODS: The prime solutions were prepared with either pasteurized human albumin or fresh frozen plasma. The total antioxidant capacity was measured with the total radical antioxidant parameter assay and with the ferric-reducing ability of plasma assay. The individual scavengers vitamin C, sulfhydryl groups, uric acid, and total protein were measured before, during, and after the prime preparation. Malondialdehyde was measured as a parameter for lipid peroxidation. RESULTS: Neither prime solution showed a total radical antioxidant parameter value. The ferric-reducing ability of plasma value of prime solutions was lower than that of undiluted human albumin or fresh frozen plasma. Addition of mannitol did not increase the ferric-reducing ability of plasma value. Vitamin C was only found in the fresh frozen plasma prime. Both prime solutions contained sulfhydryl groups and uric acid in low concentrations. During ultrafiltration, low-molecular-weight antioxidants were lost into the ultrafiltrate. CONCLUSIONS: We showed that prime solutions based on either albumin or fresh frozen plasma had very low antioxidant capacity and that ultrafiltration of the prime solution further lowers this capacity. A prime solution with a low antioxidant capacity may increase oxidative stress in neonates undergoing cardiopulmonary bypass.

Cerebral blood flow velocity wave form as an indicator of neonatal left ventricular heart function.

OBJECTIVE: To investigate the relationship between cerebral blood flow velocity wave form (CBFV-WF) parameters and myocardial contractility indices in healthy and sick preterm and term newborns. METHODS: Total group of 82 babies was divided into four subgroups: prematures with gestational age <34 weeks with (n=20) and without (n=14) respiratory distress syndrome (RDS) and infants with gestational age of >33 weeks with (n=18) and without (n=30) asphyxia. On day 1, 2, 3, 6 and 14, the acceleration time, Q(ECG)-peak(flow) time and preejection period of CBFV-WF (internal carotid artery) were measured through the anterior fontanel by Doppler ultrasonography. Simultaneously cardiac output, fractional shortening, systolic time intervals and their ratio were determined echocardiographically. RESULTS: RDS-babies had higher cardiac output and better myocardial performance then non-RDS-babies. Asphyxiated babies had lower cardiac output as compared to healthy babies, improving over time. Correlations were found between CBFV-WF parameters and several myocardial function indicators, but preejection period of cerebral blood flow velocity correlated closest with the same period measured echocardiographically (r=0.67, P<0.0001). Multiple linear regression revealed no influence of gestational age or clinical condition on this relationship. Assessment of agreement indicated that only substantial changes in myocardial performance could be monitored using preejection period of CBFV. CONCLUSION: Although a relationship was detected between the preejection period of CBFV and left ventricular systolic time interval (used as indicator of changes in left ventricular function), only rather large changes in left ventricular performance can be reliably detected using the preejection period of CBFV.

A regionalized perinatal continuing education programme: successful adaptation to a foreign health care system and language.

Much of the decline in perinatal mortality over the past two decades in the United States has been attributed to regionalization of perinatal care. Outreach education from regional medical centres to community hospitals is an essential component of regionalization. The Perinatal Continuing Education Program (PCEP) has been successfully used for outreach education in more than 30 states since 1979. This project tested the efficacy of implementing the PCEP strategy in Poland. PCEP was adapted to Polish conditions, translated, and implemented in four phases. The scheme allowed gradual transfer of ownership to Polish leaders and use of the existing regional structure to disseminate information from regional centres to community hospitals. Evaluation included measures of programme use (participation and completion rates) and acceptance (participant evaluation forms), cognitive knowledge (pre- vs. post-tests), and patient care (chart reviews). Of 2093 doctors, nurses and midwives who began, 1615 (77%) completed the programme, with higher completion by regional centre than community hospital staff. All participant groups responded favourably to the materials and expressed moderate confidence in their mastery of the information and skills. Test scores improved significantly for all phases and for all disciplines, with baseline and final scores consistent with degrees of previous professional education. Large baseline and inter-hospital variations in chart review data restricted analysis of care practices. A comprehensive perinatal education programme can be successfully transferred to a foreign health care system. We believe the following to be particularly important: multidisciplinary instructors and students; a self-instructional format; content aimed at practice rather than theory; and an organized implementation strategy co-ordinated by local personnel.